ELM Details - LIG_14-3-3

LIG_14-3-3_3

Functional site class:	14-3-3 ligand
Functional site description:	14-3-3 proteins interact with phosphoserine or phosphothreonine containing motifs.
ELM(s):	LIG_14-3-3_1, LIG_14-3-3_2, LIG_14-3-3_3
LIG_14-3-3_3 description:	Consensus derived from natural interactors which do not exactly match the mode1 and mode2 ligands
Pattern:	[RHK][STALV].[ST].[PESRDIF]
Present in taxon(s):	Homo sapiens Schizosaccharomyces pombe Nicotiana tabacum Mus musculus Rattus norvegicus Xenopus laevis Eukaryota Arabidopsis thaliana Drosophila melanogaster Saccharomyces cerevisiae
Not represented in taxon(s):

See instances for LIG_14-3-3_3

Abstract

The 14-3-3 proteins constitute a family of conserved proteins present in all eukaryotic organisms so far investigated, including seven isotypes in human cells. They are involved in important cellular processes such as signal transduction, cell-cycle control, apoptosis, stress response and malignant transformation. More than one hundred different binding partners for these proteins have been reported yet. 14-3-3 proteins were the first signaling molecules to be identified as discrete phosphoserine/threonine binding modules, although cases of phosphorilation-independent interaction has been reported. Although the exact function of 14-3-3 proteins is still unknown, they are known to act as adaptor molecules to mediate protein-protein interactions and the subcellular localisation and to regulate enzymes activity. Though 14-3-3 proteins perform different functions for different ligands, general mechanisms of 14-3-3 action include changes in activity of bound ligands, altered association of bound ligands with other cellular components, and changes in intracellular localization of 14-3-3-bound cargo.

Selected references

Brunet A, Bonni A, Zigmond MJ, Lin MZ, Juo P, Hu LS, Anderson MJ, Arden KC, Blenis J, Greenberg ME

Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor.

Cell 1999 Mar 19;96(6) : 857-68.

PMID: 10102273

Cacace AM, Michaud NR, Therrien M, Mathes K, Copeland T, Rubin GM, Morrison DK

Identification of constitutive and ras-inducible phosphorylation sites of KSR: implications for 14-3-3 binding, mitogen-activated protein kinase binding, and KSR overexpression.

Mol Cell Biol 1999 Jan;19(1) : 229-40.

PMID: 9858547

Hausser A, Storz P, Link G, Stoll H, Liu YC, Altman A, Pfizenmaier K, Johannes FJ

Protein kinase C mu is negatively regulated by 14-3-3 signal transduction proteins.

J Biol Chem 1999 Apr 2;274(14) : 9258-64.

PMID: 10092600

Kuwana T, Peterson PA, Karlsson L

Exit of major histocompatibility complex class II-invariant chain p35 complexes from the endoplasmic reticulum is modulated by phosphorylation.

Proc Natl Acad Sci U S A 1998 Feb 3;95(3) : 1056-61.

PMID: 9448284

Liu YC, Liu Y, Elly C, Yoshida H, Lipkowitz S, Altman A

Serine phosphorylation of Cbl induced by phorbol ester enhances its association with 14-3-3 proteins in T cells via a novel serine-rich 14-3-3-binding motif.

J Biol Chem 1997 Apr 11;272(15) : 9979-85.

PMID: 9092538

Ogihara T, Isobe T, Ichimura T, Taoka M, Funaki M, Sakoda H, Onishi Y, Inukai K, Anai M, Fukushima Y, Kikuchi M, Yazaki Y, Oka Y, Asano T

14-3-3 protein binds to insulin receptor substrate-1, one of the binding sites of which is in the phosphotyrosine binding domain.

J Biol Chem 1997 Oct 3;272(40) : 25267-74.

PMID: 9312143

Zhang SH, Kobayashi R, Graves PR, Piwnica-Worms H, Tonks NK

Serine phosphorylation-dependent association of the band 4.1-related protein-tyrosine phosphatase PTPH1 with 14-3-3beta protein.

J Biol Chem 1997 Oct 24;272(43) : 27281-7.

PMID: 9341175

Zhou Y, Schopperle WM, Murrey H, Jaramillo A, Dagan D, Griffith LC, Levitan IB

A dynamically regulated 14-3-3, Slob, and Slowpoke potassium channel complex in Drosophila presynaptic nerve terminals.

Neuron 1999 Apr;22(4) : 809-18.

PMID: 10230800

This ELM has been assigned the following Gene Ontology (GO) terms for biological process, cellular component and molecular function.

Biological Process

  cell proliferation

GO:0008283

  cell cycle control

GO:0000074

  protein kinase cascade

GO:0007243

  apoptotic program

GO:0008632

Cellular Component

  internal side of plasma membrane

GO:0009898

  nucleus

GO:0005634

  cytosol

GO:0005829

Molecular Function

  protein domain specific binding

GO:0019904

Instances for LIG_14-3-3_3

Sequence	Position	Subsequence (Click for evidence information)	PDB	Gene Name	Protein Description	Organism
SLOB_DROME	51-56	ANGLKRSNSAIEFDVSA	-	Name=Slob; ORFNames=CG6772;	Slowpoke-binding protein.	Drosophila melanogaster (Fruit fly).
SLOB_DROME	76-81	IYSSNRSASSEQDNSDL	-	Name=Slob; ORFNames=CG6772;	Slowpoke-binding protein.	Drosophila melanogaster (Fruit fly).
IRS1_HUMAN	267-272	DEFRPRSKSQSSSNCSN	-	Name=IRS1;	Insulin receptor substrate 1 (IRS-1).	Homo sapiens (Human).
IRS1_HUMAN	371-376	HPPLNHSRSIPMPASRC	-	Name=IRS1;	Insulin receptor substrate 1 (IRS-1).	Homo sapiens (Human).
IRS1_HUMAN	638-643	MPMSPKSVSAPQQIINP	-	Name=IRS1;	Insulin receptor substrate 1 (IRS-1).	Homo sapiens (Human).
FOXO3_HUMAN	29-34	PQSRPRSCTWPLQRPEL	-	Name=FOXO3; Synonyms=FKHRL1, FOXO3A;	Forkhead box protein O3 (Forkhead in rhabdomyosarcoma-like 1) (AF6q21 protein).	Homo sapiens (Human).
FOXO3_HUMAN	250-255	KAPRRRAVSMDNSNKYT	-	Name=FOXO3; Synonyms=FKHRL1, FOXO3A;	Forkhead box protein O3 (Forkhead in rhabdomyosarcoma-like 1) (AF6q21 protein).	Homo sapiens (Human).
RIM15_YEAST	1072-1077	LLDISRSSTPPLANPTN	-	Name=RIM15; Synonyms=TAK1; OrderedLocusNames=YFL033C;	Serine/threonine-protein kinase RIM15 (EC 2.7.11.1).	Saccharomyces cerevisiae (Baker's yeast).
HDAC4_HUMAN	629-634	HRPLSRAQSSPASATFP	-	Name=HDAC4; Synonyms=KIAA0288;	Histone deacetylase 4 (HD4).	Homo sapiens (Human).
KSR1_MOUSE	294-299	FPTLTRSKSHESQLGNR	-	Name=Ksr1; Synonyms=Ksr;	Kinase suppressor of Ras 1 (mKSR1) (Protein Hb).	Mus musculus (Mouse).
KSR1_MOUSE	389-394	LARLRRTESVPSDINNP	-	Name=Ksr1; Synonyms=Ksr;	Kinase suppressor of Ras 1 (mKSR1) (Protein Hb).	Mus musculus (Mouse).
PTN3_HUMAN	356-361	NPAMRRSLSVEHLETKS	-	Name=PTPN3; Synonyms=PTPH1;	Tyrosine-protein phosphatase non-receptor type 3 (EC 3.1.3.48) (Protein-tyrosine phosphatase H1) (PTP-H1).	Homo sapiens (Human).
PTN3_HUMAN	832-837	YVRSLRVDSEPVLVHCS	-	Name=PTPN3; Synonyms=PTPH1;	Tyrosine-protein phosphatase non-receptor type 3 (EC 3.1.3.48) (Protein-tyrosine phosphatase H1) (PTP-H1).	Homo sapiens (Human).