The Eukaryotic Linear Motif resource for
Functional Sites in Proteins
Accession:
Functional site class:
EVH1 ligands
Functional site description:
Proline-rich sequences that bind to the signal transduction modules EVH1.
ELMs with same func. site: LIG_EVH1_1  LIG_EVH1_2  LIG_EVH1_3 
ELM Description:
Proline-rich motif binding to signal transduction class I EVH1 domains.
Pattern: ([FYWL]P.PP)|([FYWL]PP[ALIVTFY]P)
Pattern Probability: 0.0001237
Present in taxons: Caenorhabditis elegans Drosophila melanogaster Eumetazoa Gallus gallus Homo sapiens Mus musculus
Interaction Domain:
WH1 (PF00568) WH1 domain (Stochiometry: 1 : 1)
PDB Structure: 1EVH
o See 19 Instances for LIG_EVH1_1
o Abstract
Enabled/vasodilator-stimulated phosphoprotein homology 1 (EVH1) domains are 115 residue protein-protein interaction modules which provide essential links for their host proteins to various signal transduction pathways. Many EVH1-containing proteins are associated closely with actin-based structures and are involved in re-organization of the actin cytoskeleton. EVH1 domains are also present in proteins enriched in neuronal tissue, thus implicating them as potential mediators of synaptic plasticity, linking them to memory formation and learning. EVH1 domains recognize and bind specific proline-rich sequences (PRSs). In general, a small (3-6 residue) core PRS in the target protein binds a 'recognition pocket' on the domain surface. Further affinity- and specificity-increasing interactions are then formed between additional domain epitopes and peptide 'core-flanking' residues. The protein-protein interactions mediated by EVH1 domains are clearly highly important for the regulation of signal transduction events, re-organization of the actin cytoskeleton, and modulation of actin dynamics and actin-based motility.
EVH1 domains can be divided into three classes based on the consensus sequences of their target PRS ligands. Single residue peptide substitution experiments have shown the consensus binding motif for class I EVH1 domains to be F-P-X-(hydrophobic)-P. Such sequences are found in a number of mammalian proteins, including the focal adhesion proteins zyxin and vinculin, as well as in the ActA protein of the intracellular pathogen Listeria monocytogenes. Class II EVH1 domains are found in the Homer-Vesl protein family of postsynaptic receptor associated proteins. These recognize a consensus PRS, with a core motif comprising PPxxF, found in the group I mGluRs, IP3Rs, RyRs and the Shank family proteins. The EVH1 domains exclusive for WASP and N-WASP proteins are also called WH1/ WASP-homology 1 domains. The EVH1 class III motifs are found in the verprolin family of proteins. They are known to play a role in WASP/N-WASP- mediated processes (e.g. regulation actin filament dynamics by WIP and WIRE), yet their exact functions remain to be elucidated. The recognition motif of EVH1 class III includes a polyproline region, which is a common feature of all EVH1 motifs, yet in class III it comprises an additional region which contains two highly conserved phenylalanines.
o 7 selected references:

o 10 GO-Terms:

o 19 Instances for LIG_EVH1_1
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
Acc., Gene-, NameStartEndSubsequenceLogic#Ev.OrganismNotes
Q702N8 XIRP1
XIRP1_HUMAN
22 26 RMATAEDLPLPPPPALEDLP TP 5 Homo sapiens (Human)
3 
2 
Q15942 ZYX
ZYX_HUMAN
71 75 PPPPPEDFPLPPPPLAGDGD TP 2 Homo sapiens (Human)
P33379 actA
ACTA_LISMO
300 304 SEPSSFEFPPPPTDEELRLA TP 5 Listeria monocytogenes
3 
P33379 actA
ACTA_LISMO
265 269 EEVNASDFPPPPTDEELRLA TP 4 Listeria monocytogenes
3 
P33379 actA
ACTA_LISMO
335 339 SEPSSFEFPPPPTEDELEII TP 3 Listeria monocytogenes
2 
Q12955 ANK3
ANK3_HUMAN
3254 3258 NRVAYIEFPPPPPLDADQIE TP 1 Homo sapiens (Human)
1 
P33379 actA
ACTA_LISMO
380 384 HSQNFSDFPPIPTEEELNGR TP 2 Listeria monocytogenes
2 
P12003 VCL
VINC_CHICK
842 846 FQPQEPDFPPPPPDLEHLHL TP 1 Gallus gallus (Chicken)
1 
P18206 VCL
VINC_HUMAN
842 846 FQPQEPDFPPPPPDLEQLRL TP 3 Homo sapiens (Human)
2 
Q04584 ZYX
ZYX_CHICK
102 106 SFSSNCAFPPPPPPFEEPFP TP 2 Gallus gallus (Chicken)
1 
Q04584 ZYX
ZYX_CHICK
113 117 PPPFEEPFPPAPDEAFPSPP TP 2 Gallus gallus (Chicken)
1 
Q04584 ZYX
ZYX_CHICK
121 125 PPAPDEAFPSPPPPPPPMFD TP 2 Gallus gallus (Chicken)
1 
Q15942 ZYX
ZYX_HUMAN
104 108 PPPIEESFPPAPLEEEIFPS TP 1 Homo sapiens (Human)
1 
Q15942 ZYX
ZYX_HUMAN
114 118 APLEEEIFPSPPPPPEEEGG TP 1 Homo sapiens (Human)
1 
Q15942 ZYX
ZYX_HUMAN
93 97 EGALGGAFPPPPPPIEESFP TP 1 Homo sapiens (Human)
1 
O15117 FYB
FYB_HUMAN
616 620 QSGSGGIFPPPPDDDIYDGI TP 2 Homo sapiens (Human)
1 
P51617 IRAK1
IRAK1_HUMAN
168 172 VPSPASLWPPPPSPAPSSTK TP 2 Homo sapiens (Human)
1 
Q9Y6N7 ROBO1
ROBO1_HUMAN
1186 1190 GMNWADLLPPPPAHPPPHSN TP 2 Homo sapiens (Human)
1 
Q15942 ZYX
ZYX_HUMAN
73 77 PPPEDFPLPPPPLAGDGDDA TP 2 Homo sapiens (Human)
1 
Please cite: ELM 2016-data update and new functionality of the eukaryotic linear motif resource. (PMID:26615199)

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