The Eukaryotic Linear Motif resource for
Functional Sites in Proteins
Functional site class:
Clathrin box
Functional site description:
Clathrin boxes are found on cargo adaptor proteins. They interact with the N-terminal beta-propeller structure of Clathrin heavy chain. Beside the "classical" motif, some variants have been described for various types of adaptor proteins. The same protein can bear several subtypes of Clathrin boxes.
ELMs with same func. site: LIG_Clathr_ClatBox_1  LIG_Clathr_ClatBox_2 
ELM Description:
The classical clathrin box is a motif found on cargo adaptor proteins, in general near their C-terminus. This motif is sufficient for binding to the amino terminal beta propeller structure of Clathrin heavy chain. Variants of this "classical" consensus exist (see LIG_Clathr_ClatBox_2 for example) sometimes they coexist on the same protein.
Pattern: L[IVLMF].[IVLMF][DE]
Pattern Probability: 0.0003406
Present in taxons: Eukaryota Homo sapiens Mus musculus Rattus norvegicus Saccharomyces cerevisiae
Interaction Domain:
Clathrin_propel (PF01394) Clathrin propeller repeat (Stochiometry: 1 : 1)
PDB Structure: 1C9L
o See 18 Instances for LIG_Clathr_ClatBox_1
o Abstract
Clathrin is the main component of the coat that decorates the cytosolic face of carrier vesicles mediating protein and lipid transport in the endocytic and late secretory pathways. In vivo the multimerisation of the clathrin heterohexamer (known as triskelion) and the clathrin-mediated vesicular trafficking are regulated through a complex network of interactions involving clathrin and a number of accessory proteins.
Clathrin boxes are 5-residue-motifs found on cargo adaptor proteins, mostly near their C-terminus or central region. They are found in a large variety of accessory proteins involved in endocytosis and vesicular trafficking (specific adaptors like beta-arrestin and more general adaptor proteins like AP complexes). Unfolded flexible regions or hinges can display multiple copies of the motif, sometime belonging to different subtypes. This organisation is believed to ensure a complex, ajustable network of "weak" cooperative interactions, with the affinity between members defined by the number of motifs they possess. Extended peptides could also promote clathrin assembly by bridging the heavy chains of clathrin molecules engaged in an assembled coat. Clathin box motifs interact with the N-terminus of Clathrin heavy chain, with the so called beta-propeller structure.
o 6 selected references:

o 8 GO-Terms:

o 18 Instances for LIG_Clathr_ClatBox_1
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
Acc., Gene-, NameStartEndSubsequenceLogic#Ev.OrganismNotes
Q9VI75 lap
192 196 LQAQLDALLEFDCQSNDLSN FP 1 Drosophila melanogaster (Fruit fly)
O88339 Epn1
480 484 FLGPNAALVDLDSLVSRPGP TP 2 Rattus norvegicus (Norway rat)
Q91VZ6 Smap1
192 196 AAEPTIDLLGLDGPAEAPVT TP 6 Mus musculus (House mouse)
Q05140 Snap91
178 182 LQGQIDALLEFDVHPNELTN FP 3 Rattus norvegicus (Norway rat)
O00499 BIN1
123 127 QKLVDQALLTMDTYLGQFPD FP 1 Homo sapiens (Human)
P49418 AMPH
118 122 QKLVDGSLLTLDTYLGQFPD FP 1 Homo sapiens (Human)
349 353 GCMKTCPLIDLEVDNGPAQM TP 4 Homo sapiens (Human)
Q91YI4 Arrb2
374 378 DVPVDTNLIEFDTNYATDDD TP 4 Mus musculus (House mouse)
O00291 HIP1
332 336 PVLEKDDLMDMDASQQNLFD TP 6 Homo sapiens (Human)
Q13367 AP3B2
808 812 PATKEISLLDLEDFTPPSVQ TP 5 Homo sapiens (Human)
P49418 AMPH
351 355 EVKKEETLLDLDFDPFKPEV TP 3 Homo sapiens (Human)
O00499 BIN1
390 394 PFSEQASLLDLDFDPLPPVT TP 4 Homo sapiens (Human)
Q27974 DNAJC6
496 500 APPEDVDLLGLEGSAVSKNF TP 2 Bos taurus (Cattle)
P17870 ARRB1
376 380 ETPVDTNLIELDTNDDDIVF TP 1 Bos taurus (Cattle)
Q10567 AP1B1
632 636 QGDLLGDLLNLDLGPPVSGP TP 1 Homo sapiens (Human)
P63009 AP2B1
631 635 QGDLLGDLLNLDLGPPVNVP TP 1 Bos taurus (Cattle)
P32120 ARRB2
384 388 DAPVDTNLIEFETNYATDDD TP 1 Bos taurus (Cattle)
O00203 AP3B1
818 822 PLTKDVSLLDLDDFNPVSTP TP 1 Homo sapiens (Human)
Please cite: The Eukaryotic Linear Motif resource: 2022 release. (PMID:34718738)

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