The Eukaryotic Linear Motif resource for
Functional Sites in Proteins
Functional site class:
PDZ ligands
Functional site description:
The best characterised PDZ ligands are short C-terminal peptides that bind in a surface groove by beta-augmentation to a beta sheet with the PDZ domain. The peptide carboxy terminus is specifically recognised by complementary polar interactions with the PDZ domain. Although there is a considerable literature on internal sequence peptide interactions, we are not currently able to represent internal PDZ-binding peptides in ELM.
ELMs with same func. site: LIG_PDZ_Class_1  LIG_PDZ_Class_2  LIG_PDZ_Class_3 
ELM Description:
PDZ domains recognize short sequences at the carboxy terminus of target proteins. The terminal residue is apparently always hydrophobic with the -3 position being a strong determinant of specificity. The class 2 motif has a pattern such as (VYF)X(VIL)*. We have made the conserved positions more relaxed based on experimental binding data. However, probably not all PDZ domain instances can accept either A or F at the terminal position. Several less conserved positions in the motif may modulate affinity and specificity of the ligand domain interaction.
Pattern: ...[VLIFY].[ACVILF]$
Pattern Probability: 0.0000789
Present in taxons: Eukaryota Homo sapiens Metazoa Mus musculus
Interaction Domain:
PDZ (PF00595) PDZ domain (Also known as DHR or GLGF) (Stochiometry: 1 : 1)
PDB Structure: 1ZUB
o See 13 Instances for LIG_PDZ_Class_2
o Abstract
PDZ domains are ~90 residue globular protein modules that can be found in eukaryotic regulatory proteins. They are found in most eukaryotes but the domain family is hugely expanded in the Metazoa (~200 PDZ domain instances are found in the Human proteome). Thus nearly all of the metazoan PDZ domain functions are specific to this evolutionary lineage.

PDZ domain-containing proteins spend at least part of their time in membrane-associated complexes. Many PDZ ligands are themselves membrane proteins. Several PDZ domain containing proteins include multiple domain copies (MPDZ/MUPP1 and MAGI2 contain 13 and 6 PDZ domain instances respectively), acting as scaffolds, recruiting multiple PDZ domain binding proteins and facilitating the construction of large membrane-associated complexes. PDZ domains also co-occur regularly with other signalling/regulatory domains regulating many biological processes such as transport and signal transduction (Lee,2010). There is increasing evidence that some, ultimately perhaps most, PDZ domains also bind to phospholipid headgroups (Gallardo,2010). Thus PDZ domains have a role in assembly of signalling complexes at membrane locations determined by the appropriate lipid modification state. In large multiprotein complexes, they may do this in conjunction with other lipid-sampling domains such as C2, PH, PX and so forth to differentiate different membrane contexts. Peptide and lipid binding by PDZ domains is found to be co-operative, enabling tight integration of lipid and protein regulatory signals.

The known PDZ-binding specificities are divided into three classes. The position 0 (C-terminal residue) is always hydrophobic while the class is determined by the position -2 residue. These two positions are the most buried in the bound complexes, hence they are strong specificity determinants. The neighbouring residues can undoubtedly contribute to specificity and affinity of the interactions. In ELM we have chosen relaxed motif patterns based on the solved PDZ complexes, so it is likely that any given peptide will only bind to a subset of the PDZ domains belonging to that class.
o 9 selected references:

o 6 GO-Terms:

o 13 Instances for LIG_PDZ_Class_2
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
Acc., Gene-, NameStartEndSubsequenceLogic#Ev.OrganismNotes
P97288-3 Htr4
366 371 NGSTHVLSFPLLFRNRPVPV TP 3 Mus musculus (House mouse)
1638 1643 QQSSKSGHKKQKNKDREYYV TP 1 Homo sapiens (Human)
Q07310 Nrxn3
1573 1578 QASSKSGHKKQKNKDKEYYV TP 1 Rattus norvegicus (Norway rat)
P31431 SDC4
193 198 SYDLGKKPIYKKAPTNEFYA TP 3 Homo sapiens (Human)
P04626 ERBB2
1250 1255 TFKGTPTAENPEYLGLDVPV TP 2 Homo sapiens (Human)
Q13136 PPFIA1
1197 1202 DGNVSGTQRLDSATVRTYSC TP 1 Homo sapiens (Human)
P04921 GYPC
123 128 LQGDPALQDAGDSSRKEYFI TP 2 Homo sapiens (Human)
P19491 Gria2
878 883 QNFATYKEGYNVYGIESVKI TP 1 Rattus norvegicus (Norway rat)
Q811U3 Erc1
943 948 SNQTNHKPSPDQDEEEGIWA TP 2 Rattus norvegicus (Norway rat)
P55284 Cdh5
779 784 PRFKMLAELYGSDPQEELII TP 3 Mus musculus (House mouse)
Q02410 APBA1
832 837 KTMPAAMYRLLTAQEQPVYI TP 3 Homo sapiens (Human)
P49768 PSEN1
462 467 ATDYLVQPFMDQLAFHQFYI TP 5 Homo sapiens (Human)
P18827 SDC1
305 310 PKQANGGAYQKPTKQEEFYA TP 3 Homo sapiens (Human)
Please cite: The eukaryotic linear motif resource - 2018 update. (PMID:29136216)

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