ELM - instance LIG_14-3-3_CanoR_1 in sequence P08965 at position 524

Instance

Accession	Acc. Gene-, Name	Start	End	Subsequence	Logic	PDB	Organism	Length
ELMI003028	P08965 mei2 MEI2_SCHPO	524	529	TGSLGMRRSLTVGANASCSN	TP	---	Schizosaccharomyces pombe 972h-	750

Instance evidence

Evidence class	PSI-MI	Method	BioSource	PubMed	Logic	Reliability	Notes
experimental	MI:0007	anti tag coimmunoprecipitation	in vivo	Sato,2002	support	certain	InteractionDetection
experimental	MI:0018	two hybrid	in vivo	Sato,2002	support	certain	InteractionDetection
experimental	MI:0405	competition binding	in vitro	Sato,2002	support	certain	InteractionDetection
experimental	MI:0074	mutation analysis	in vivo/in vitro	Sato,2002	support	certain	FeatureDetection

Switches

This ELM instance is part of the following 2 switching mechanisms annotated at the switches.ELM resource:

SWTI000326:

Binding of meiRNA meiotic non-coding RNA (meiRNA) to the RRM domains of Meiosis protein mei2 (mei2) is essential for promotion of premeiotic DNA synthesis and meiosis I and is blocked by Pat1-mediated phosphorylation-induced binding of the 14-3-3 protein DNA damage checkpoint protein rad24 (rad24) to 2 14-3-3 binding motifs in mei2.
SWTI000459:

Phosphorylation of two 14-3-3-binding motifs in Meiosis protein mei2 (mei2) by Negative regulator of sexual conjugation and meiosis (ran1) induces high-avidity binding to dimeric DNA damage checkpoint protein rad24 (rad24), with pT527 being the high-affinity interaction site.

Please cite: The Eukaryotic Linear Motif resource: 2022 release. (PMID:34718738)

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