The Eukaryotic Linear Motif resource for
Functional Sites in Proteins
Functional site class:
AP2 alpha ligands
Functional site description:
Motifs responsible for the binding of accessory endocytic proteins to the alpha-subunit of adaptor protein AP-2 and their recruitment to the site of clathrin coated vesicle formation.
ELMs with same func. site: LIG_AP2alpha_1  LIG_AP2alpha_2 
ELM Description:
The FxDxF motif is an alpha appendage binding sequence found in several regulatory and accessory proteins essential to clathrin coat assembly. It is involved in the binding of amphipysin, AP180 and synaptojanin170 to the alpha-subunit of AP-2.
Pattern: F.D.F
Pattern Probability: 0.0000169
Present in taxons: Eukaryota Homo sapiens Mus musculus Rattus norvegicus Saccharomyces cerevisiae
Interaction Domain:
Alpha_adaptin_C (PF02296) Alpha adaptin AP2, C-terminal domain (Stochiometry: 1 : 1)
PDB Structure: 1KY7
o See 11 Instances for LIG_AP2alpha_1
o Abstract
Vesicular traffic in the endocytic and secretory pathway is mediated by vesicles that carry cargo proteins. Clathrin-coated vesicles are responsible for a large fraction of the vescicular traffic that reaches the endosomal compartment, originating from the plasma membrane or from the TGN (trans-Golgi network). The assembly of the clathrin-coated vesicles is mediated by proteic adaptors, monomeric as AP180 and CALM or multimeric complexes like AP (Adaptor Protein) complexes. AP complexes are heterotetrameric proteins that simultaneously interact with clathrin and endocytic proteins: for example, the beta-subunit of AP-2 complex binds to clathrin, the mu-subunit interacts with Y-based sorting signal present in the cytosolic tails of membrane receptors and the alpha-subunit of AP-2 binds regulatory/accessory proteins involved in the control of clathrin coated vesicle formation.
o 4 selected references:

o 7 GO-Terms:

o 11 Instances for LIG_AP2alpha_1
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
Acc., Gene-, NameStartEndSubsequenceLogic#Ev.OrganismNotes
O08838 Amph
324 328 QENIINFFEDNFVPEINVTT TP 5 Rattus norvegicus (Norway rat)
O00499 BIN1
362 366 QEQILSLFEDTFVPEISVTT TP 1 Homo sapiens (Human)
Q61548 Snap91
640 644 SSGVIDLFGDAFGSGASETQ TP 5 Mus musculus (House mouse)
Q61548 Snap91
562 566 APPALDIFGDLFDSAPEVAA TP 1 Mus musculus (House mouse)
Q61548 Snap91
436 440 VTTEVDLFGDAFAASPGEAP TP 1 Mus musculus (House mouse)
O60641 SNAP91
642 646 SSGVIDLFGDAFGSSASEPQ TP 1 Homo sapiens (Human)
O60641 SNAP91
564 568 APPALDIFGDLFESTPEVAA TP 1 Homo sapiens (Human)
O60641 SNAP91
440 444 VTAEVDLFGDAFAASPGEAP TP 1 Homo sapiens (Human)
P50478 AMPH
324 328 QENIINLFDDNFVPEINVTT TP 0 Gallus gallus (Chicken)
P49418 AMPH
324 328 QENIISFFEDNFVPEISVTT TP 1 Homo sapiens (Human)
Q9PTK7 snap91
434 438 STAEVDLFGDAFAASPDEAT TP 0 Xenopus laevis (African clawed frog)
Please cite: The Eukaryotic Linear Motif resource: 2022 release. (PMID:34718738)

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