The Eukaryotic Linear Motif resource for
Functional Sites in Proteins

146 different methods used in ELM annotation

The following table holds all methods which have been used during the ELM rigourous data curation process. Furthermore, the number of ELM instances for which experimental evidence has been annotated are listed. We use methods from, and provide linkout to, the PsiMi (Proteomics Standards Initiative - Molecular Interaction) controlled vocabulary.

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IDPSIMI IDMethodBiosourceInteraction#InstancesNotes
98 MI:0004 Affinity Chromatography Technology in vivo/in vitro association 55 InteractionDetection
9 MI:0005 Alanine Scanning in vivo/in vitro/in silico 482 FeatureDetection
460 MI:0793 Amorph in vivo/in vitro/in silico association 7
281 MI:0905 Amplified Luminescent Proximity Homogeneous Assay 20 InteractionDetection
259 MI:0006 Anti Bait Coimmunoprecipitation 20 InteractionDetection
37 MI:0257 Antisense RNA in vivo 3 InteractionDetection
67 MI:0007 Anti Tag Coimmunoprecipitation in vivo association 173 InteractionDetection
183 MI:0872 Atomic Force Microscopy 2 InteractionDetection
380 MI:0833 Autoradiography 24 ParticipantIdentification FeatureDetection
277 MI:0010 Beta Galactosidase Complementation 10 InteractionDetection
309 MI:0809 Bimolecular Fluorescence Complementation 8 InteractionDetection
156 MI:0969 Biolayer Interferometry 13 InteractionDetection
279 MI:0012 Bioluminescence Resonance Energy Transfer 1 InteractionDetection
327 MI:0968 Biosensor 10 InteractionDetection
472 MI:0239 Biotin Tag in vivo/in vitro direct interaction 4 InteractionDetection
432 MI:0276 Blue Native Page 1 InteractionDetection
458 MI:2163 By Homology in silico association 20 ParticipantIdentification
203 MI:0225 Chromatin Immunoprecipitation Array 5 InteractionDetection
104 MI:0402 Chromatin Immunoprecipitation Assay in vivo association 4 InteractionDetection
137 MI:0091 Chromatography Technology in vitro physical association 44 InteractionDetection
18 MI:0016 Circular Dichroism in vitro association 24 InteractionDetection
65 MI:0017 Classical Fluorescence Spectroscopy in vitro association 132 InteractionDetection
405 MI:0990 Cleavage Assay 10 InteractionDetection
129 MI:0194 Cleavage Reaction in vivo/in vitro 50 InteractionDetection
23 MI:0019 Coimmunoprecipitation in vivo/in vitro association 932 InteractionDetection
16 MI:0403 Colocalization in vitro 207
146 MI:0807 Comigration In Gel Electrophoresis in vitro 10 InteractionDetection
123 MI:0404 Comigration In Non Denaturing Gel Electrophoresis in vivo association 12 InteractionDetection
132 MI:0808 Comigration In Sds Page in vitro 45 InteractionDetection
12 MI:0405 Competition Binding in vitro physical association 277 InteractionDetection
35 MI:0663 Confocal Microscopy in vivo/in vitro colocalization 133 InteractionDetection
145 MI:0027 Cosedimentation in vitro physical association 11 InteractionDetection
42 MI:0030 Cross Linking Study in vivo/in vitro direct interaction 17 InteractionDetection
134 MI:1006 Deglycosylase Assay in vivo/in vitro 33 InteractionDetection
321 MI:0033 Deletion Analysis 104 FeatureDetection
31 MI:0032 De Novo Protein Sequencing By Mass Spectrometry in vivo/in vitro association 12 ParticipantIdentification FeatureDetection
408 MI:0995 Depalmitoylase Assay 1 InteractionDetection
30 MI:0943 Detection By Mass Spectrometry in vivo/in vitro association 242 InteractionDetection
198 MI:1311 Differential Scanning Calorimetry 5 InteractionDetection
7 MI:0034 Display Technology in vivo/in vitro association 15 InteractionDetection
135 MI:0039 Edman Degradation in vitro 63 ParticipantIdentification
50 MI:0040 Electron Microscopy in vivo/in vitro association 41 InteractionDetection
345 MI:0042 Electron Paramagnetic Resonance 1 InteractionDetection FeatureDetection
436 MI:0982 Electrophoretic Mobility-based Method 2 InteractionDetection
205 MI:0413 Electrophoretic Mobility Shift Assay 21 InteractionDetection
147 MI:0412 Electrophoretic Mobility Supershift Assay association 9 InteractionDetection
131 MI:0411 Elisa in vitro direct interaction 51 InteractionDetection ParticipantIdentification
471 MI:0414 Enzymatic Reaction in vivo/in vitro enzymatic reaction 2 FeatureDetection
348 MI:0045 Experimental Interaction Detection 24 InteractionDetection
464 MI:0206 Farnesylation Reaction in vitro enzymatic reaction 4 InteractionDetection
83 MI:0047 Far Western Blotting in vitro association 100 InteractionDetection
57 MI:1022 Field Flow Fractionation in vitro colocalization 1 InteractionDetection
342 MI:0049 Filter Binding 23 InteractionDetection
60 MI:0054 Fluorescence-activated Cell Sorting in vivo association 15 InteractionDetection
152 MI:0416 Fluorescence Microscopy colocalization 96 InteractionDetection
61 MI:0053 Fluorescence Polarization Spectroscopy in vitro association 170 InteractionDetection
365 MI:1016 Fluorescence Recovery After Photobleaching 2 InteractionDetection
143 MI:0051 Fluorescence Technology direct interaction 70 InteractionDetection
144 MI:0055 Fluorescent Resonance Energy Transfer direct interaction 20 InteractionDetection
469 MI:2286 Functional Association in vivo/in vitro association 12 InteractionDetection ParticipantIdentification
468 MI:0240 Fusion Protein in vivo/in vitro colocalization 68 InteractionDetection ParticipantIdentification
149 MI:0728 Gal4 Vp16 Complementation in vitro physical association 8 InteractionDetection
390 MI:1003 Geranylgeranylase Assay 1 InteractionDetection
465 MI:0209 Geranylgeranylation Reaction in vitro enzymatic reaction 2
91 MI:0519 Glutathione S Tranferase Tag in vitro 719
56 MI:0367 Green Fluorescent Protein Tag in vivo 85
459 MI:0521 His Tag in vivo/in vitro/in silico association 54
216 MI:0421 Identification By Antibody 29 ParticipantIdentification
214 MI:0427 Identification By Mass Spectrometry 140 ParticipantIdentification
212 MI:0428 Imaging Technique 1 InteractionDetection
141 MI:0858 Immunodepleted Coimmunoprecipitation in vitro physical association 5 InteractionDetection
112 MI:0363 Inferred By Author in silico association 114 InteractionDetection ParticipantIdentification FeatureDetection
312 MI:0364 Inferred By Curator in silico association 23 InteractionDetection ParticipantIdentification FeatureDetection
218 MI:0423 In-gel Kinase Assay 14 InteractionDetection
148 MI:0586 Inhibitor 117
24 MI:0064 Interologs in silico association 15 InteractionDetection
202 MI:0226 Ion Exchange Chromatography 1 InteractionDetection
47 MI:0065 Isothermal Titration Calorimetry in vitro association 446 InteractionDetection
463 MI:0253 Isotope Label in vivo/in vitro enzymatic reaction 8
28 MI:0788 Knock Out in vivo 43
397 MI:0067 Light Scattering 4 InteractionDetection
383 MI:0729 Luminescence Based Mammalian Interactome Mapping 2 InteractionDetection
86 MI:0068 Mass Detection Of Residue Modification in vitro 34 FeatureDetection
400 MI:0069 Mass Spectrometry Studies Of Complexes 5 InteractionDetection
106 MI:0944 Mass Spectrometry Study Of Hydrogen/deuterium Exchange in vivo/in vitro association 3 InteractionDetection
466 0213 Methylation Reaction in vitro enzymatic reaction 7 InteractionDetection
457 MI:1247 Microscale Thermophoresis in vitro direct interaction 12 InteractionDetection
32 MI:0071 Molecular Sieving in vivo/in vitro colocalization 19 InteractionDetection
329 MI:0818 Molecular Weight Estimation By Coomasie Staining 16 ParticipantIdentification
427 MI:0072 Monoclonal Antibody Western Blot 34 ParticipantIdentification FeatureDetection
8 MI:0074 Mutation Analysis in vivo/in vitro 2080 FeatureDetection
470 MI:0119 Mutation Decreasing Interaction in vivo/in vitro direct interaction 25 InteractionDetection
100 MI:0573 Mutation Disrupting Interaction in vivo/in vitro 161
138 MI:1002 Myristoylase Assay in vivo/in vitro 2 InteractionDetection
150 MI:0429 Necessary Binding Site 16
246 MI:0929 Northern Blot 2 ParticipantIdentification
22 MI:0077 Nuclear Magnetic Resonance in vitro association 237 InteractionDetection FeatureDetection
142 MI:1089 Nuclear Translocation Assay in vivo 2 InteractionDetection
36 MI:0506 Over Expressed Level in vivo 40
17 Overlapping Binding Peptide in vitro 2
282 MI:0900 P8 Filamentous Phage Display 52 InteractionDetection
154 MI:0081 Peptide Array 86 InteractionDetection
63 Peptide Binding To Perpetrator in vitro 3
162 MI:0083 Peptide Synthesis 1 ParticipantIdentification
161 MI:0084 Phage Display 23 InteractionDetection
224 MI:1088 Phenotype-based Detection Assay 172 InteractionDetection
94 MI:0434 Phosphatase Assay in vitro enzymatic reaction 120 InteractionDetection
273 MI:1297 Phosphoamino Acid Analysis 12 FeatureDetection
53 MI:0086 Polyclonal Antibody in vitro 18 ParticipantIdentification FeatureDetection
157 MI:0435 Protease Assay 3 InteractionDetection
151 MI:0089 Protein Array direct interaction 40 InteractionDetection
26 MI:0570 Protein Cleavage in vivo/in vitro 12
73 MI:0424 Protein Kinase Assay in vitro enzymatic reaction 323 InteractionDetection
72 MI:0424 Protein Kinase Assay in vivo enzymatic reaction 257 InteractionDetection
27 MI:0424 Protein Kinase Assay in vivo/in vitro enzymatic reaction 27 InteractionDetection
69 MI:0723 Protein Microinjection in vivo 1
363 MI:1019 Protein Phosphatase Assay 2 InteractionDetection
197 MI:1314 Proximity-dependent Biotin Identification 2 InteractionDetection
337 MI:0813 Proximity Ligation Assay 9 InteractionDetection ParticipantIdentification
3 MI:0096 Pull Down in vivo/in vitro association 1138 InteractionDetection
461 1260 Pyrene Label in vitro direct interaction 4 InteractionDetection
291 MI:1197 Radioimmunoassay 7 ParticipantIdentification
139 MI:0517 Radiolabel in vitro physical association 61
140 MI:0256 Rna Interference in vivo 42 InteractionDetection
136 MI:0099 Scintillation Proximity Assay in vitro physical association 2 InteractionDetection
14 MI:0101 Sequence Based Prediction in silico association 434 InteractionDetection
242 MI:0102 Sequence Tag Identification 1 ParticipantIdentification
370 MI:1234 Silac 9 FeatureDetection
133 MI:0892 Solid Phase Assay in vitro physical association 10 InteractionDetection
454 MI:1203 Split Luciferase Complementation 2 InteractionDetection
15 MI:0105 Structure Based Prediction in silico association 44 InteractionDetection
71 MI:0372 Subcellular Preparation in vitro 14
335 MI:0810 Substitution Analysis 3 FeatureDetection
153 MI:1008 Sumoylase Assay 3 InteractionDetection
467 MI:2213 Super-resolution Microscopy in vivo/in vitro association 2 ParticipantIdentification
5 MI:0107 Surface Plasmon Resonance in vitro association 193 InteractionDetection FeatureDetection
165 MI:0867 Tag Visualisation By Fluorescence 45 ParticipantIdentification
395 MI:1220 Tag Visualisation By Luciferase Assay 8 ParticipantIdentification
155 MI:0676 Tandem Affinity Purification 36 InteractionDetection
473 MI:0677 Tandem Tag in vivo/in vitro association 2 InteractionDetection
107 Two Dimensional Phosphopeptide Mapping in vitro 21
54 MI:0018 Two Hybrid in vivo association 528 InteractionDetection
93 MI:0997 Ubiquitinase Assay in vitro enzymatic reaction 71 InteractionDetection
55 MI:0113 Western Blot in vitro direct interaction 567 ParticipantIdentification FeatureDetection
4 MI:0114 X-ray Crystallography in vitro association 592 InteractionDetection FeatureDetection
357 MI:0826 X Ray Scattering 10 InteractionDetection
Please cite: ELM-the Eukaryotic Linear Motif resource-2024 update. (PMID:37962385)

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