The Eukaryotic Linear Motif resource for
Functional Sites in Proteins
Accession:
Functional site class:
Siah binding Motif
Functional site description:
The members of the SINA/Siah family have domain architecture consisting of a RING domain, two zinc finger motifs and a substrate/adaptor binding domain (SBD) that mediates the interaction with the binding partners.
ELM Description:
The PxAxVxP motif was first described to confer a high-affinity binding to the Siah/PHYL interaction. It has been detected in other Siah interacting proteins, including DCC, KLF10, OBF-1. The motif binds by partial Beta-augmentation. The present pattern is stricter than proposed by House,2006. It will find most verified instances but may miss some candidates.
Pattern: .P.A.V.P[^P]
Pattern Probability: 0.0000271
Present in taxon: Metazoa
Interaction Domain:
Sina (PF03145) Seven in absentia protein family (Stochiometry: 1 : 1)
PDB Structure: 2AN6
o See 9 Instances for DEG_SIAH_1
o Abstract
Proteasomal degradation of proteins plays important roles in cell physiology. In eukaryotic cells, this process involves the specific covalent modification by a highly conserved small regulatory protein, ubiquitin, which labels target proteins for proteolysis and subsequent degradation. Ubiquitination reaction is carried out by the E1 (ubiquitin activating proteins) - E2 (ubiquitin conjugating proteins) - E3 (ubiquitin ligases) cascade of enzymes. The specificity for targeting substrates for ubiquitination is mainly conferred by the E3 proteins. Siah proteins are members of an evolutionarily highly conserved family of E3 ubiquitin ligases which regulate the ubiquitination and proteasome-dependent degradation of several proteins. The mammalian Siah genes are homologues of the Drosophila SINA (seven in absentia homologue), which is required for the R7 cell determination in the developing eyes, and they are characterized by the presence of a RING finger domain that is responsible for E3 activity.
SIAH/sina proteins interact with several proteins and participate in the regulation of some of them through ubiquitination and proteasome-dependent degradation. Degraded proteins include Drosophila repressor Tramtrack (Ttk88) (Li,1997; Tang,1997), netrin-1 receptor deleted in colorectal cancer (DCC) (Hu,1997), coactivator BOB.1/OBF.1 (Boehm,2001; Tiedt,2001), nuclear receptor corepressor (NcoR) (Zhang,1998), the transcriptional repressor TIEG-1 (Johnsen,2002) and Kid (Germani,2001), a protein necessary for chromosome movement during mitosis and meiosis. SIAH-1 also interacts with a complex including step1, Ebi, Sip (SIAH-interacting protein) and APC (adenomatosis polyposis colonic protein), which facilitates the degradation of beta-catenin in a p53-dependent manner (Liu,2001; Matsuzawa,2001). The C-terminus region of the Siah proteins has been demonstrated to be a substrate- and cofactor- interaction module (Substrate-Binding Domain, SBD. The X-ray analysis of the Siah SBD revealed an eight-stranded beta sandwich fold (Polekhina,2002). House and colleagues (House,2003) have identified a binding motif PxAxVxP (named Siah degron), present in the Drosophila protein PHYL, which binds with high affinity to the SINA and Siah SBDs. The degron motif is present in several Siah degradation targets. The structure of the Siah1 in complex with a degron-containing peptide from the adaptor protein Phyllopod has been recently solved (2AN6) (House,2006). Other Siah-interacting proteins, including KID, APC, synphylin-1, carry similar peptides that do not perfectly match the reported consensus degron motif.
o 6 selected references:

o 8 GO-Terms:

o 9 Instances for DEG_SIAH_1
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
Acc., Gene-, NameStartEndSubsequenceLogic#Ev.OrganismNotes
Q13118 KLF10
KLF10_HUMAN
200 208 AARKNIPCAAVSPNRSKCER TP 1 Homo sapiens (Human)
Q27934 phyl
PHYL_DROME
115 123 ERTKLRPVAMVRPTVRVQPQ TP 5 Drosophila melanogaster (Fruit fly)
Q7Z6J0 SH3RF1
SH3R1_HUMAN
600 608 AHNQERPTAAVTPIQVQNAA TP 2 Homo sapiens (Human)
Q16633 POU2AF1
OBF1_HUMAN
46 54 SGAAPAPTAVVLPHQPLATY TP 2 Homo sapiens (Human)
Q86TG7 PEG10
PEG10_HUMAN
253 261 RKPRSPPRALVLPHIASHHQ TP 2 Homo sapiens (Human)
O75553 DAB1
DAB1_HUMAN
360 368 VMGAQPPVAQVMPGAQPIAW TP 2 Homo sapiens (Human)
Q9HB71 CACYBP
CYBP_HUMAN
59 67 LLDNEKPAAVVAPITTGYTV TP 4 Homo sapiens (Human)
Q9UHB7 AFF4
AFF4_HUMAN
252 260 NSMLQKPTAYVRPMDGQESM TP 2 Homo sapiens (Human)
P43146 DCC
DCC_HUMAN
1331 1339 APSRTIPTACVRPTHPLRSF TP 1 Homo sapiens (Human)
Please cite: ELM 2016-data update and new functionality of the eukaryotic linear motif resource. (PMID:26615199)

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