The Eukaryote Linear Motif resource for Functional Sites in Proteins
Accession:
Functional site class:
PKB Phosphorylation site
Functional site description:
Motif phosphorylated by a subset of AGC group kinases including PKB that all have similar sequence specificity.
ELM Description:
Phosphorylation site preference R.R.(ST) targeted by some basophilic kinases of the AGC group, including PKB and p70S6K. AGC group kinases do not tolerate Pro at position +1. Lys at the Arg positions may be allowed in some weaker sites. Some specificity determinants may occur at the less conserved non-basic sites: e.g Phe at position +2 is reported to be an optimal PKB site but a poor one for p60S6K.
Pattern: R.R..([ST])[^P]..
Pattern Probability: 0.0006034
Present in taxons: Caenorhabditis elegans Dictyostelium discoideum Drosophila melanogaster Eukaryota Vertebrata
Interaction Domain:
Pkinase (PF00069) Protein kinase domain (Stochiometry: 1 : 1)
o See 20 Instances for MOD_PKB_1
o Abstract
Protein kinase B (PKB/Akt/Rac-protein kinase) belongs to the large set of related AGC kinases having a preference for phosphorylating basophilic sites. PKB is a key player in the signaling pathways of numerous essential eukaryotic cellular processes including glucose metabolism, cell proliferation, apoptosis, cell migration and transcription. Many targets of PKB have been identified in vitro, and many studies looking at in vivo roles are currently underway. Kinase activity of PKB is stimulated by a variety of growth factors, cytokines, chemokines, heat shock, hyperosmolarity, hypoxia, integrin engagement and T-cell receptor interactions. The N-termini of the protein contains a pleckstrin homology (PH) domain that binds lipid products of phosphoinositide 3'-kinase (PI3K) and mediates recruitment to the membrane in response to PI3K (Obata,2000). Translocation of PKB then allows phosphorylation at Thr-308 by another Ser/Thr protein kinase (3-phosphoinositide-dependent protein kinase 1 (PDK1)) and full activation occurs after phosphorylation of Ser-473 (Toker,2000). A serine (or less often a threonine) residue at the end of the motif in PKB substrates is then phosphorylated.
o 7 selected references:

o 2 GO-Terms:

o 20 Instances for MOD_PKB_1
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
Acc., Gene-, NameStartEndSubsequenceLogic#Ev.OrganismNotes
P12813 Nr4a1
NR4A1_MOUSE
349 357 SLKGRRGRLPSKPKQPPDAS TP 1 Mus musculus (House mouse)
O14746 TERT
TERT_HUMAN
819 827 CHHAVRIRGKSYVQCQGIPQ TP 1 Homo sapiens (Human)
P26285 PFKFB2
F262_BOVIN
462 470 SQTPVRMRRNSFTPLSSSNT TP 1 Bos taurus (Cattle)
P98177 FOXO4
FOXO4_HUMAN
192 200 SGKAPRRRAASMDSSSKLLR TP 3 Homo sapiens (Human)
P46527 CDKN1B
CDN1B_HUMAN
152 160 QCAGIRKRPATDDSSTQNKR TP 6 Homo sapiens (Human)
1 
P38936 CDKN1A
CDN1A_HUMAN
140 148 GDSQGRKRRQTSMTDFYHSK TP 2 Homo sapiens (Human)
1 
Q61337 Bad
BAD_MOUSE
131 139 ELSPFRGRSRSAPPNLWAAQ TP 5 Mus musculus (House mouse)
1 
P98177 FOXO4
FOXO4_HUMAN
27 35 FEPQSRPRSCTWPLPRPEIA TP 3 Homo sapiens (Human)
1 
Q9WV60 Gsk3b
GSK3B_MOUSE
4 12 MSGRPRTTSFAESCKPVQQP TP 2 Mus musculus (House mouse)
1 
P22736 NR4A1
NR4A1_HUMAN
346 354 SLKGRRGRLPSKPKQPPDAS TP 2 Homo sapiens (Human)
1 
P55211 CASP9
CASP9_HUMAN
191 199 DCEKLRRRFSSLHFMVEVKG TP 2 Homo sapiens (Human)
1 
O15111 CHUK
IKKA_HUMAN
18 26 GPWEMRERLGTGGFGNVCLY TP 2 Homo sapiens (Human)
1 
Q00987 MDM2
MDM2_HUMAN
161 169 PSTSSRRRAISETEENSDEL TP 2 Homo sapiens (Human)
1 
Q00987 MDM2
MDM2_HUMAN
181 189 SGERQRKRHKSDSISLSFDE TP 2 Homo sapiens (Human)
1 
P38398 BRCA1
BRCA1_HUMAN
504 512 TNKLKRKRRPTSGLHPEDFI TP 2 Homo sapiens (Human)
1 
P04049 RAF1
RAF1_HUMAN
254 262 GSLSQRQRSTSTPNVHMVST TP 2 Homo sapiens (Human)
1 
P49815 TSC2
TSC2_HUMAN
934 942 EKDSFRARSTSLNERPKSLR TP 1 Homo sapiens (Human)
1 
P49815 TSC2
TSC2_HUMAN
976 984 AAEAFRCRSISVSEHVVRSR TP 1 Homo sapiens (Human)
1 
P49815 TSC2
TSC2_HUMAN
1127 1135 RGARDRVRSMSGGHGLRVGA TP 1 Homo sapiens (Human)
1 
P49815 TSC2
TSC2_HUMAN
1457 1465 SPSGLRPRGYTISDSAPSRR TP 1 Homo sapiens (Human)
1 
Please cite: The Eukaryotic Linear Motif Resource ELM: 10 Years and Counting (PMID:24214962)

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