The Eukaryote Linear Motif resource for Functional Sites in Proteins
Functional site class:
PKA Phosphorylation site
Functional site description:
Motifs phosphorylated by a subset of AGC group kinases including PKA that all have similar sequence specificity.
ELMs with this model: MOD_PKA_1  MOD_PKA_2 
Description:
Secondary preference for PKA-type AGC kinase phosphorylation with a single Arg at p-2. This motif is probably more often targeted by other basophilic kinases of the AGC group, including PAK1 and PKC isoforms: These kinases actually show a stronger preference at p-2 than PKA, which has the strongest basophilic preference at p-3. AGC group kinases do not tolerate Pro at position +1. It is likely that some specificity determinants distinguishing among these kinases may be present at the less conserved non-basic sites.
Pattern: .R.([ST])[^P]..
Pattern Probability: 0.0094575
Present in taxon: Eukaryota
Interaction Domain:
Pkinase (PF00069) Protein kinase domain (Stochiometry: 1 : 1)
o See 28 Instances for MOD_PKA_2
o Abstract
PKA belongs to the large set of related AGC kinases having a preference for phosphorylating basophilic sites. cAMP-dependent protein kinase A (PKA) is the major target for cAMP action in eukaryotic cells. The enzyme is allosterically activated by binding of cAMP to two regulatory (R) subunits, which induces dissociation of two catalytic (C) subunits. The active kinase is then free to phosphorylate substrates on serine, or less commonly threonine residues in recognition motifs. The enzyme acts on a wide number of proteins involved in several biochemical processes. These include glucose metabolism (via activation and inactivation of glycogen phosphorylase b and glycogen synthase, respectively), fatty acid synthesis (via acetyl-coA carboxylase), and pyruvate oxidation (via pyruvate dehyrogenase complex). This is therefore a vital signaling enzyme in a number of metabolic processes, affecting many cell functions by activating or inactivating substrate proteins in the presence of cAMP.
o 4 selected references:

o 4 GO-Terms:

o 28 Instances for MOD_PKA_2
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
Protein NameGene NameStartEndSubsequenceLogic#Ev.OrganismNotes
SCN5A_RAT Scn5a 523 529 SRTSMRPRSSRGSIFTFRRR TP 6 Rattus norvegicus (Norway rat)
CSKP_RAT Cask 559 565 QKMLREMRGSITFKIVPSYR TP 4 Rattus norvegicus (Norway rat)
SCN5A_RAT Scn5a 526 532 SMRPRSSRGSIFTFRRRDQG TP 6 Rattus norvegicus (Norway rat)
THOP1_RAT Thop1 641 647 PKVGMDYRTSILRPGGSEDA TP 6 Rattus norvegicus (Norway rat)
NOXA1_HUMAN NOXA1 458 464 FVVPAGPRMSGAPGRLPRSQ TP 3 Homo sapiens (Human)
AKT2_ARATH AKT2 207 213 TRLEKDIRYSYFWIRCFRLL TP 1 Arabidopsis thaliana (Thale cress)
NEB2_RAT Ppp1r9b 91 97 RASDRGVRLSLPRASSLNEN TP 5 Rattus norvegicus (Norway rat)
AKT2_ARATH AKT2 326 332 TRRTMEFRNSIEAASNFVNR TP 1 Arabidopsis thaliana (Thale cress)
IRK1_HUMAN KCNJ1 310 316 TSATCQVRTSYVPEEVLWGY TP 4 Homo sapiens (Human)
PELP1_HUMAN PELP1 412 418 LNSWSIGRDSLSPGQERPYS TP 5 Homo sapiens (Human)
NEB2_RAT Ppp1r9b 174 180 RRLLRQERASLQDRKLDVVV TP 4 Rattus norvegicus (Norway rat)
SNAPN_RAT Snapin 47 53 DSHVHAVRESQVELREQIDN TP 6 Rattus norvegicus (Norway rat)
B9EIS4_HUMAN GRK1 18 24 NSAFIAARGSFDGSSSQPSR TP 4 Homo sapiens (Human)
Q26658_STRPU Stage specifi 84 90 DCKRCKARGSEKRMGPGDQR TP 6 Strongylocentrotus purpuratus
HSPB8_HUMAN HSPB8 54 60 WPDWALPRLSSAWPGTLRSG TP 2 Homo sapiens (Human)
BIRC5_HUMAN BIRC5 17 23 QPFLKDHRISTFKNWPFLEG TP 4 Homo sapiens (Human)
GDIR1_HUMAN ARHGDIA 171 177 APKGMLARGSYSIKSRFTDD TP 4 Homo sapiens (Human)
IRK1_HUMAN KCNJ1 216 222 LIRVANLRKSLLIGSHIYGK TP 4 Homo sapiens (Human)
EF2K_RAT Eef2k 496 502 PSRLHLPRPSAVALEVQRLN TP 4 Rattus norvegicus (Norway rat)
RXRA_HUMAN RXRA 24 30 SLTSPTGRGSMAAPSLHPSL TP 3 Homo sapiens (Human)
MEF2D_MOUSE Mef2d 187 193 PQQPALQRNSVSPGLPQRPA TP 6 Mus musculus (House mouse)
C2TA_HUMAN CIITA 831 837 VVQELPGRLSFLGTRLTPPD TP 5 Homo sapiens (Human)
C2TA_HUMAN CIITA 1047 1053 SLAASLLRLSLYNNCICDVG TP 5 Homo sapiens (Human)
PLSL_HUMAN LCP1 2 8 MARGSVSDEEMMELREAFAK TP 6 Homo sapiens (Human)
PKD1_MOUSE Pkd1 4156 4162 PLPSRSSRGSKSSPVVLPPS TP 3 Mus musculus (House mouse)
CCG2_MOUSE Cacng2 317 323 QKDSKDSLHANTANRRTTPV TP 3 Mus musculus (House mouse)
NFAC1_HUMAN NFATC1 242 248 HSPSTSPRASVTEESWLGAR TP 5 Homo sapiens (Human)
NFAC1_HUMAN NFATC1 291 297 PSPHGSPRVSVTDDSWLGNT TP 5 Homo sapiens (Human)
Please cite: The Eukaryotic Linear Motif Resource ELM: 10 Years and Counting (PMID:24214962)

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