The Eukaryote Linear Motif resource for Functional Sites in Proteins
Functional site class:
PKA Phosphorylation site
Functional site description:
Motifs phosphorylated by a subset of AGC group kinases including PKA that all have similar sequence specificity.
ELMs with this model: MOD_PKA_1  MOD_PKA_2 
Description:
The optimal phosphorylation site preference for PKA is RR.(ST) - Lysine in the basic sites may weaken the prefence. This site is also targeted by other basophilic kinases of the AGC group, including PKG and PKC isoforms. AGC group kinases do not tolerate Pro at position +1. Lys at the Arg positions may be allowed in some weaker sites. Some specificity determinants may be present at the less conserved non-basic sites.
Pattern: [RK][RK].([ST])[^P]..
Pattern Probability: 0.0023151
Present in taxon: Eukaryota
Interaction Domain:
Pkinase (PF00069) Protein kinase domain (Stochiometry: 1 : 1)
o See 25 Instances for MOD_PKA_1
o Abstract
PKA belongs to the large set of related AGC kinases having a preference for phosphorylating basophilic sites. cAMP-dependent protein kinase A (PKA) is the major target for cAMP action in eukaryotic cells. The enzyme is allosterically activated by binding of cAMP to two regulatory (R) subunits, which induces dissociation of two catalytic (C) subunits. The active kinase is then free to phosphorylate substrates on serine, or less commonly threonine residues in recognition motifs. The enzyme acts on a wide number of proteins involved in several biochemical processes. These include glucose metabolism (via activation and inactivation of glycogen phosphorylase b and glycogen synthase, respectively), fatty acid synthesis (via acetyl-coA carboxylase), and pyruvate oxidation (via pyruvate dehyrogenase complex). This is therefore a vital signaling enzyme in a number of metabolic processes, affecting many cell functions by activating or inactivating substrate proteins in the presence of cAMP.
o 6 selected references:

o 5 GO-Terms:

o 25 Instances for MOD_PKA_1
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
Protein NameGene NameStartEndSubsequenceLogic#Ev.OrganismNotes
KAP2_BOVIN PRKAR2A 93 99 IPGRFDRRVSVCAETYNPDE TP 1 Bos taurus (Cattle)
AIBP_MOUSE Apoa1bp 40 46 IWWGTQRRGSETMAGAAVKY TP 3 Mus musculus (House mouse)
AICDA_HUMAN AICDA 24 30 VRWAKGRRETYLCYVVKRRD TP 3 Homo sapiens (Human)
AICDA_HUMAN AICDA 35 41 LCYVVKRRDSATSFSLDFGY TP 3 Homo sapiens (Human)
RARA_HUMAN RARA 366 372 KVYVRKRRPSRPHMFPKMLM TP 4 Homo sapiens (Human)
HDAC8_HUMAN HDAC8 36 42 SLAKIPKRASMVHSLIEAYA TP 5 Homo sapiens (Human)
PTBP1_HUMAN PTBP1 13 19 DIAVGTKRGSDELFSTCVTN TP 5 Homo sapiens (Human)
RIMS1_RAT Rims1 410 416 SRARHERRHSDVALPHTEAA TP 5 Rattus norvegicus (Norway rat)
CSKP_RAT Cask 721 727 VKLPAFKRKTLVLLGAHGVG TP 4 Rattus norvegicus (Norway rat)
NOXA1_HUMAN NOXA1 169 175 ALDQVQRRGSLPPRQVPRGE TP 4 Homo sapiens (Human)
CTNB1_HUMAN CTNNB1 549 555 AHQDTQRRTSMGGTQQQFVE TP 4 Homo sapiens (Human)
CTNB1_HUMAN CTNNB1 672 678 KPQDYKKRLSVELTSSLFRT TP 4 Homo sapiens (Human)
LASP1_MOUSE Lasp1 153 159 QDSSSYRRPTEQQQPQPHHI TP 3 Mus musculus (House mouse)
AT1A1_RAT Atp1a1 940 946 LVICKTRRNSVFQQGMKNKI TP 3 Rattus norvegicus (Norway rat)
STK11_MOUSE Stk11 428 434 PARKVCSSNKIRRLSACKQQ TP 4 Mus musculus (House mouse)
KPYK1_YEAST CDC19 19 25 VAGSDLRRTSIIGTIGPKTN TP 3 Saccharomyces cerevisiae (Baker"s yeast)
MCLN1_HUMAN MCOLN1 554 560 PTSGKFRRGSGSACSLLCCC TP 3 Homo sapiens (Human)
PTN5_MOUSE Ptpn5 157 163 PLPPEDRRQSVSRQPSFTYS TP 3 Mus musculus (House mouse)
PTN5_MOUSE Ptpn5 218 224 SMGLQERRGSNVSLTLDMCT TP 3 Mus musculus (House mouse)
SOX9_HUMAN SOX9 61 67 KGEPDLKKESEEDKFPVCIR TP 5 Homo sapiens (Human)
RGRF1_MOUSE Rasgrf1 913 919 SNKEVFRRMSLANTGFSSDQ TP 4 Mus musculus (House mouse)
MERL_HUMAN NF2 515 521 FKDTDMKRLSMEIEKEKVEY TP 4 Homo sapiens (Human)
MEF2D_MOUSE Mef2d 118 124 LLEDKYRRASEELDGLFRRY TP 6 Mus musculus (House mouse)
NMDZ1_HUMAN GRIN1 894 900 ASSFKRRRSSKDTSTGGGRG TP 4 Homo sapiens (Human)
NFAC1_HUMAN NFATC1 266 272 ASPCNKRKYSLNGRQPPYSP TP 5 Homo sapiens (Human)
Please cite: The Eukaryotic Linear Motif Resource ELM: 10 Years and Counting (PMID:24214962)

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