The Eukaryote Linear Motif resource for Functional Sites in Proteins
Accession:
Functional site class:
PKA Phosphorylation site
Functional site description:
Motifs phosphorylated by a subset of AGC group kinases including PKA that all have similar sequence specificity.
ELMs with same func. site: MOD_PKA_1  MOD_PKA_2 
ELM Description:
The optimal phosphorylation site preference for PKA is RR.(ST) - Lysine in the basic sites may weaken the prefence. This site is also targeted by other basophilic kinases of the AGC group, including PKG and PKC isoforms. AGC group kinases do not tolerate Pro at position +1. Lys at the Arg positions may be allowed in some weaker sites. Some specificity determinants may be present at the less conserved non-basic sites.
Pattern: [RK][RK].([ST])[^P]..
Pattern Probability: 0.0023151
Present in taxon: Eukaryota
Interaction Domain:
Pkinase (PF00069) Protein kinase domain (Stochiometry: 1 : 1)
o See 25 Instances for MOD_PKA_1
o Abstract
PKA belongs to the large set of related AGC kinases having a preference for phosphorylating basophilic sites. cAMP-dependent protein kinase A (PKA) is the major target for cAMP action in eukaryotic cells. The enzyme is allosterically activated by binding of cAMP to two regulatory (R) subunits, which induces dissociation of two catalytic (C) subunits. The active kinase is then free to phosphorylate substrates on serine, or less commonly threonine residues in recognition motifs. The enzyme acts on a wide number of proteins involved in several biochemical processes. These include glucose metabolism (via activation and inactivation of glycogen phosphorylase b and glycogen synthase, respectively), fatty acid synthesis (via acetyl-coA carboxylase), and pyruvate oxidation (via pyruvate dehyrogenase complex). This is therefore a vital signaling enzyme in a number of metabolic processes, affecting many cell functions by activating or inactivating substrate proteins in the presence of cAMP.
o 6 selected references:

o 5 GO-Terms:

o 25 Instances for MOD_PKA_1
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
Acc., Gene-, NameStartEndSubsequenceLogic#Ev.OrganismNotes
P00515 PRKAR2A
KAP2_BOVIN
93 99 IPGRFDRRVSVCAETYNPDE TP 1 Bos taurus (Cattle)
Q8K4Z3 Apoa1bp
AIBP_MOUSE
40 46 IWWGTQRRGSETMAGAAVKY TP 3 Mus musculus (House mouse)
Q9GZX7 AICDA
AICDA_HUMAN
24 30 VRWAKGRRETYLCYVVKRRD TP 3 Homo sapiens (Human)
Q9GZX7 AICDA
AICDA_HUMAN
35 41 LCYVVKRRDSATSFSLDFGY TP 3 Homo sapiens (Human)
P10276 RARA
RARA_HUMAN
366 372 KVYVRKRRPSRPHMFPKMLM TP 4 Homo sapiens (Human)
Q9BY41 HDAC8
HDAC8_HUMAN
36 42 SLAKIPKRASMVHSLIEAYA TP 5 Homo sapiens (Human)
P26599 PTBP1
PTBP1_HUMAN
13 19 DIAVGTKRGSDELFSTCVTN TP 5 Homo sapiens (Human)
Q9JIR4 Rims1
RIMS1_RAT
410 416 SRARHERRHSDVALPHTEAA TP 5 Rattus norvegicus (Norway rat)
Q62915 Cask
CSKP_RAT
721 727 VKLPAFKRKTLVLLGAHGVG TP 4 Rattus norvegicus (Norway rat)
Q86UR1 NOXA1
NOXA1_HUMAN
169 175 ALDQVQRRGSLPPRQVPRGE TP 4 Homo sapiens (Human)
P35222 CTNNB1
CTNB1_HUMAN
549 555 AHQDTQRRTSMGGTQQQFVE TP 4 Homo sapiens (Human)
P35222 CTNNB1
CTNB1_HUMAN
672 678 KPQDYKKRLSVELTSSLFRT TP 4 Homo sapiens (Human)
Q61792 Lasp1
LASP1_MOUSE
153 159 QDSSSYRRPTEQQQPQPHHI TP 3 Mus musculus (House mouse)
P06685 Atp1a1
AT1A1_RAT
940 946 LVICKTRRNSVFQQGMKNKI TP 3 Rattus norvegicus (Norway rat)
Q9WTK7 Stk11
STK11_MOUSE
428 434 PARKVCSSNKIRRLSACKQQ TP 4 Mus musculus (House mouse)
P00549 CDC19
KPYK1_YEAST
19 25 VAGSDLRRTSIIGTIGPKTN TP 3 Saccharomyces cerevisiae (Baker"s yeast)
Q9GZU1 MCOLN1
MCLN1_HUMAN
554 560 PTSGKFRRGSGSACSLLCCC TP 3 Homo sapiens (Human)
P54830 Ptpn5
PTN5_MOUSE
157 163 PLPPEDRRQSVSRQPSFTYS TP 3 Mus musculus (House mouse)
P54830 Ptpn5
PTN5_MOUSE
218 224 SMGLQERRGSNVSLTLDMCT TP 3 Mus musculus (House mouse)
P48436 SOX9
SOX9_HUMAN
61 67 KGEPDLKKESEEDKFPVCIR TP 5 Homo sapiens (Human)
P27671 Rasgrf1
RGRF1_MOUSE
913 919 SNKEVFRRMSLANTGFSSDQ TP 4 Mus musculus (House mouse)
P35240 NF2
MERL_HUMAN
515 521 FKDTDMKRLSMEIEKEKVEY TP 4 Homo sapiens (Human)
Q63943 Mef2d
MEF2D_MOUSE
118 124 LLEDKYRRASEELDGLFRRY TP 6 Mus musculus (House mouse)
Q05586 GRIN1
NMDZ1_HUMAN
894 900 ASSFKRRRSSKDTSTGGGRG TP 4 Homo sapiens (Human)
O95644 NFATC1
NFAC1_HUMAN
266 272 ASPCNKRKYSLNGRQPPYSP TP 5 Homo sapiens (Human)
Please cite: The Eukaryotic Linear Motif Resource ELM: 10 Years and Counting (PMID:24214962)

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