The Eukaryote Linear Motif resource for Functional Sites in Proteins
MOD_PIKK_1 202
 
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Functional site class: PIKK phosphorylation site
Functional site description: The phosphoinositide-3-OH-kinase related kinases (PIKKs) are atypical protein kinases exclusive to eukaryotes.The PIKK members are large proteins with Ser/Thr kinase activity serving important roles in DNA repair and DNA damage checkpoints. The three PIKK proteins with repair and checkpoint functions in mammalian cells are: DNA-PK (DNA-dependent protein kinase),ATM (ataxia telangiectasia mutated), and ATR (ATM and Rad3 related) .
ELMs: MOD_PIKK_1
Description: The PIKK family member proteins specifically phosphorylate the (ST)Q motif in their substrates. The glutamine adjacent to the target serine-threonine is critical for the substrate recognition
Pattern: ...([ST])Q.. (Probability: 0.0092301)
Present in taxons: Eukaryota
Interaction Domain:

PI3_PI4_kinase (PF00454)
Phosphatidylinositol 3- and 4-kinase
(Stochiometry: 1 : 1)

o See 30 Instances for MOD_PIKK_1


o Abstract

The PIKKs are a group of ancient eukaryotic protein kinases including ATM, ATR and DNA-PK. These proteins are characterized by their large size (>200 KDa) and by the presence of a highly conserved phosphoinositide 3-kinase-like catalytic domain. They appear to function principally as protein kinases, however (Yang et al.,2003, 14716813). PIKKs are the principal components of the DNA damage checkpoint pathway and are probably present in all eukaryotes. The common phosphorylation site for PIKKs is a serine or threonine followed by a glutamine residue, a motif commonly dubbed ...[S/T]Q... (Kim et al., 1999, 10608806).Often substrate regions are characterized by high local density of SQ/TQ motifs that have been termed "SCDs"(SQ/TQ cluster domains). While sharing the same substrate specificity, the PIKK family members are activated by different checkpoint responses. ATM is involved in the checkpoint response to ionizing radiation,DNA double strand breaks and in V(D)J recombination, while ATR seems to play a primary role in checkpoints induced by UV irradiation or DNA replication blocks. DNA-PK, which has essential functions in V(D)J recombination, as well as in non-homologous end joining, is also associated with the checkpoint response (Yang et al., 2003, 14716813). In general it is often found that the same substrate is phosphorylated by both ATM and ATR in a cell cycle and time dependent manner.

o 15 selected references:

o 5 GO-Terms:

o 30 Instances for MOD_PIKK_1
(click table headers for sorting)
SequenceStartEndSubsequence
Instance LogicPDB Organism
XPA_HUMAN 193 199 VKRSLEVWGSQEALEEAKEV true positive --- Homo sapiens (Human)
GCR_RAT 524 530 GIQQATAGVSQDTSENPNKT true positive --- Rattus norvegicus (Norway rat)
BLM_HUMAN 96 102 FKNAPAGQETQRGGSKSLLP true positive --- Homo sapiens (Human)
BRCA1_HUMAN 1384 1390 VSEDCSGLSSQSDILTTQQR true positive --- Homo sapiens (Human)
BLM_HUMAN 119 125 QTPKEVVCTTQNTPTVKKSR true positive --- Homo sapiens (Human)
BRCA1_HUMAN 1420 1426 LEAVLEQHGSQPSNSYPSII true positive --- Homo sapiens (Human)
Q8AY27_XENLA 2 8 MPFLTQPEADDDQYVLTCSL true positive --- Xenopus laevis (African clawed frog)
BRCA1_HUMAN 1521 1527 GSLQNRNYPSQEELIKVVDV true positive --- Homo sapiens (Human)
ABL1_MOUSE 443 449 MSPYPGIDLSQVYELLEKDY true positive --- Mus musculus (House mouse)
NBN_HUMAN 340 346 KTTTPGPSLSQGVSVDEKLM true positive --- Homo sapiens (Human)
JUN_HUMAN 246 252 PPLSPIDMESQERIKAERKR true positive --- Homo sapiens (Human)
MCM2_HUMAN 105 111 DEDVEELTASQREAAERAMR true positive --- Homo sapiens (Human)
MDM2_HUMAN 392 398 SQSQESEDYSQPSTSSSIIY true positive --- Homo sapiens (Human)
CHK2_HUMAN 65 71 TLSSLETVSTQELYSIPEDQ true positive --- Homo sapiens (Human)
E2F1_HUMAN 28 34 AGALRLLDSSQIVIISAAQD true positive --- Homo sapiens (Human)
SRF_HUMAN 432 438 GSLTVLNAFSQAPSTMQVSH true positive --- Homo sapiens (Human)
SRF_HUMAN 443 449 APSTMQVSHSQVQEPGGVPQ true positive --- Homo sapiens (Human)
H2A1_YEAST 126 132 IHQNLLPKKSAKATKASQEL true positive --- Saccharomyces cerevisiae (Baker"s yeast)
HS90A_HUMAN 2 8 MPEETQTQDQPMEEEEVETF true positive --- Homo sapiens (Human)
HS90A_HUMAN 4 10 MPEETQTQDQPMEEEEVETF true positive --- Homo sapiens (Human)
P53_HUMAN 12 18 SDPSVEPPLSQETFSDLWKL true positive --- Homo sapiens (Human)
H2AX_MOUSE 137 143 TVGPKAPAVGKKASQASQEY true positive --- Mus musculus (House mouse)
TERF1_HUMAN 216 222 FKSKLLMIISQKDTFHSFFQ true positive --- Homo sapiens (Human)
4EBP1_RAT 108 114 HSSPEDKRAGGEESQFEMDI true positive --- Rattus norvegicus (Norway rat)
CHK1_HUMAN 314 320 SEENVKYSSSQPEPRTGLSL true positive --- Homo sapiens (Human)
CHK1_HUMAN 342 348 DKLVQGISFSQPTCPDHMLL true positive --- Homo sapiens (Human)
XRCC1_HUMAN 368 374 CAFANTPKYSQVLGLGGRIV true positive --- Homo sapiens (Human)
MDM4_HUMAN 400 406 FLDLAHSSESQETISSMGEQ true positive --- Homo sapiens (Human)
MDM2_HUMAN 404 410 STSSSIIYSSQEDVKEFERE true positive --- Homo sapiens (Human)
MLL1_HUMAN 513 519 PEVHPPLPISQSPENESNDR true positive --- Homo sapiens (Human)

Please cite: ELM - the database of eukaryotic linear motifs (PMID:22110040)

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