LIG_CtBP
ELM server details
ELM
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Functional site class:
 CtBP ligand
Functional site description:
 CtBP (C-terminal binding protein) interaction site
ELM(s): LIG_CtBP
LIG_CtBP description: PxDLS motif that interacts with the CtBP protein
Pattern: [PG][LVIPME][DENS]L[VASTRGE]
Present in taxon(s): Metazoa  
Not represented in taxon(s):

o See instances for LIG_CtBP


o Abstract

The CtBP (C-terminal binding protein) protein was first identified by Boyd at al. (1993 - PMID:8440238), where a short region in adenovirus E1A was found essential in its interaction with CtBP. Deletion of this region in E1A resulted in increased trancriptional activity, suggesting that CtBP might be a co-repressor for E1A. Several eukaryotic proteins such as Hairy, Knirps, Ikaros, Polycomb and several zinc finger proteins interact with CtBP through a short motif with the consensus PxDLS (see review PMID:11864595). CtBP is structurally related to dehydrogenases and is indeed a NAD-dependent dehydrogenase (PMID:12419229). CtBP interacts with the PxDLS motif through its dehydrogenase domain, in a NAD+ dependent manner. CtBP dimerise and can simultaneously interact with two PxDLS containing proteins, e.g. with a DNA binding protein and a repressor.

o Selected references

Chinnadurai G
CtBP, an unconventional transcriptional corepressor in development and oncogenesis.
Mol Cell 2002 Feb;9(2) : 213-24.
PMID: 11864595

Kim HY, Ahn BY, Cho Y
Structural basis for the inactivation of retinoblastoma tumor suppressor by SV40 large T antigen.
EMBO J 2001 Jan 15;20(1) : 295-304.
PMID: 11226179

Kumar V, Carlson JE, Ohgi KA, Edwards TA, Rose DW, Escalante CR, Rosenfeld MG, Aggarwal AK
Transcription corepressor CtBP is an NAD(+)-regulated dehydrogenase.
Mol Cell 2002 Oct;10(4) : 857-69.
PMID: 12419229

Turner J, Crossley M
The CtBP family: enigmatic and enzymatic transcriptional co-repressors.
Bioessays 2001 Aug;23(8) : 683-90.
PMID: 11494316

o This ELM has been assigned the following Gene Ontology (GO) terms for biological process, cellular component and molecular function.

Biological Process
  negative regulation of transcription
Cellular Component
  nucleus
Molecular Function
  transcription regulator
  transcription factor binding
  transcription co-repressor

 

o Instances for LIG_CtBP

SequencePositionSubsequence
(Click for evidence information)		PDBGene NameProtein DescriptionOrganism
HDAC9_MOUSE 23-27 LEPISPLDLRTDLRMM - Name=Hdac9; Synonyms=Hdac7b, Hdrp, Mitr; Histone deacetylase 9 (HD9) (HD7B) (Histone deacetylase-related protein) (MEF2-interacting transcription repressor MITR). Mus musculus (Mouse).
HDAC7_MOUSE 22-26 GSQPQPMDLRVGQRPT - Name=Hdac7a; Synonyms=Hdac7; Histone deacetylase 7a (HD7a). Mus musculus (Mouse).
HDAC5_HUMAN 56-60 GGSPSPVELRGALVGS - Name=HDAC5; Synonyms=KIAA0600; Histone deacetylase 5 (HD5) (Antigen NY-CO-9). Homo sapiens (Human).
HDAC4_HUMAN 48-52 APSAVPMDLRLDHQFS - Name=HDAC4; Synonyms=KIAA0288; Histone deacetylase 4 (HD4). Homo sapiens (Human).
TTKB_DROME 593-597 ASTPPPPDLSGQNSNQ - Name=ttk; Synonyms=FTZ-F2; ORFNames=CG1856; Protein tramtrack, beta isoform (Tramtrack p69) (Repressor protein fushi tarazu). Drosophila melanogaster (Fruit fly).
SOX6_HUMAN 424-428 EAAAQPLNLSSRPKTA - Name=SOX6; Transcription factor SOX-6. Homo sapiens (Human).
HIC1_HUMAN 241-245 CSPLCGLDLSKKSPPG - Name=HIC1; Synonyms=ZBTB29; Hypermethylated in cancer 1 protein (Hic-1) (Zinc finger and BTB domain-containing protein 29). Homo sapiens (Human).
ACL6B_HUMAN 51-55 AEEGGGLELEGDKEKK - Name=ACTL6B; Synonyms=ACTL6, BAF53B; Actin-like protein 6B (53 kDa BRG1-associated factor B) (Actin-related protein Baf53b) (ArpNalpha). Homo sapiens (Human).
TGIF1_HUMAN 24-28 DSMDIPLDLSSSAGSG - Name=TGIF1; Synonyms=TGIF; Homeobox protein TGIF1 (5'-TG-3'-interacting factor 1). Homo sapiens (Human).
LCOR_HUMAN 203-207 ADQDSPLDLTVRKSQS - Name=LCOR; Synonyms=KIAA1795, MLR2; Ligand-dependent corepressor (LCoR) (Mblk1-related protein 2). Homo sapiens (Human).
RBBP8_HUMAN 490-494 CVMDKPLDLSDRFSAI - Name=RBBP8; Synonyms=CTIP; Retinoblastoma-binding protein 8 (RBBP-8) (CtBP-interacting protein) (CtIP) (Retinoblastoma-interacting protein and myosin-like) (RIM). Homo sapiens (Human).
KLF3_HUMAN 61-65 GIQMEPVDLTVNKRSS - Name=KLF3; Synonyms=BKLF; Krueppel-like factor 3 (Basic krueppel-like factor) (CACCC-box-binding protein BKLF) (TEF-2). Homo sapiens (Human).
KLF8_HUMAN 86-90 LPQVEPVDLSFHKPKA - Name=KLF8; Synonyms=BKLF3, ZNF741; Krueppel-like factor 8 (Basic krueppel-like factor 3) (Zinc finger protein 741). Homo sapiens (Human).
FOG2_HUMAN 829-833 LEMDVPIDLSKKCLSQ - Name=ZFPM2; Synonyms=FOG2; Zinc finger protein ZFPM2 (Zinc finger protein multitype 2) (Friend of GATA protein 2) (FOG-2) (hFOG-2). Homo sapiens (Human).
EVI1_HUMAN 584-588 TSQDQPLDLSMGSRSR - Name=EVI1; Ecotropic virus integration site 1 protein homolog (EVI-1). Homo sapiens (Human).
IKZF1_HUMAN 34-38 EPMPIPEDLSTTSGGQ - Name=IKZF1; Synonyms=IK1, IKAROS, LYF1, ZNFN1A1; DNA-binding protein Ikaros (Ikaros family zinc finger protein 1) (Lymphoid transcription factor LyF-1). Homo sapiens (Human).
ZFH1_DROME 792-796 TREDQPLDLSVKRDPL - Name=zfh1; Synonyms=zfh-1; ORFNames=CG1322; Zinc finger protein 1 (Zinc finger homeodomain protein 1). Drosophila melanogaster (Fruit fly).
ZEB1_HUMAN 734-738 EPQVEPLDLSLPKQQG - Name=ZEB1; Synonyms=AREB6, TCF8; Zinc finger E-box-binding homeobox 1 (Transcription factor 8) (NIL-2-A zinc finger protein) (Negative regulator of IL2). Homo sapiens (Human).
HAIR_DROME 318-322 PMEQQPLSLVIKKQIK - Name=h; ORFNames=CG6494; Protein hairy. Drosophila melanogaster (Fruit fly).
ELK3_HUMAN 273-277 SDSLEPLNLSSGSKTK - Name=ELK3; Synonyms=NET, SAP2; ETS domain-containing protein Elk-3 (ETS-related protein NET) (ETS- related protein ERP) (SRF accessory protein 2) (SAP-2). Homo sapiens (Human).
KNIR_DROME 331-335 SAQEGPMDLSMKTSRS - Name=kni; Synonyms=NR0A1; ORFNames=CG4717; Zygotic gap protein knirps (Nuclear receptor subfamily 0 group A member 1). Drosophila melanogaster (Fruit fly).
NRIP1_HUMAN 440-444 YSNCVPIDLSCKHRTE - Name=NRIP1; Nuclear receptor-interacting protein 1 (Nuclear factor RIP140) (Receptor-interacting protein 140). Homo sapiens (Human).
FOG1_HUMAN 792-796 PAADGPIDLSKKPRRP - Name=ZFPM1; Synonyms=FOG1; Zinc finger protein ZFPM1 (Zinc finger protein multitype 1) (Friend of GATA protein 1) (Friend of GATA-1) (FOG-1). Homo sapiens (Human).
Q91647_XENLA 455-459 SDLDEPIDLRCVKSRC - Name=XPolycomb; XPolycomb. Xenopus laevis (African clawed frog).
CBX4_HUMAN 470-474 SDLDEPIDLRSVKSRS - Name=CBX4; E3 SUMO-protein ligase CBX4 (Chromobox protein homolog 4) (Polycomb 2 homolog) (Pc2) (hPc2). Homo sapiens (Human).
IKZF4_HUMAN 323-327 EAGEGPEDLADGGPLL - Name=IKZF4; Synonyms=KIAA1782, ZNFN1A4; Zinc finger protein Eos (Ikaros family zinc finger protein 4). Homo sapiens (Human).
TRPS1_HUMAN 1163-1167 SDNDIPLDLAIKHSRP - Name=TRPS1; Zinc finger transcription factor Trps1 (Zinc finger protein GC79) (Tricho-rhino-phalangeal syndrome type I protein). Homo sapiens (Human).
T7L1A_XENLA 469-473 SEQAQPLSLTTKPEAR - Name=tcf7l1-A; Synonyms=tcf3, tcf3a; Transcription factor 7-like 1-A (HMG box transcription factor 3-A) (TCF-3-A) (xTcf-3). Xenopus laevis (African clawed frog).
T7L1A_XENLA 545-549 LLQAQPLSLVTKSSD - Name=tcf7l1-A; Synonyms=tcf3, tcf3a; Transcription factor 7-like 1-A (HMG box transcription factor 3-A) (TCF-3-A) (xTcf-3). Xenopus laevis (African clawed frog).
ZEB2_HUMAN 785-789 SNTPSPLNLSSTSSKN - Name=ZEB2; Synonyms=KIAA0569, SIP1, ZFHX1B, ZFX1B; ORFNames=HRIHFB2411; Zinc finger E-box-binding homeobox 2 (Zinc finger homeobox protein 1b) (Smad-interacting protein 1) (SMADIP1). Homo sapiens (Human).
ZEB2_HUMAN 815-819 ELQAEPLDLSLPKQMK - Name=ZEB2; Synonyms=KIAA0569, SIP1, ZFHX1B, ZFX1B; ORFNames=HRIHFB2411; Zinc finger E-box-binding homeobox 2 (Zinc finger homeobox protein 1b) (Smad-interacting protein 1) (SMADIP1). Homo sapiens (Human).
ZEB2_HUMAN 859-863 ENSDEPLNLTFIKKEF - Name=ZEB2; Synonyms=KIAA0569, SIP1, ZFHX1B, ZFX1B; ORFNames=HRIHFB2411; Zinc finger E-box-binding homeobox 2 (Zinc finger homeobox protein 1b) (Smad-interacting protein 1) (SMADIP1). Homo sapiens (Human).
E1A_ADE02 279-283 NESGQPLDLSCKRPRP - - Early E1A 32 kDa protein. Human adenovirus 2 (HAdV-2).