| << LIG_APCC_KENbox_2 << | Menu | >> LIG_BIR_II_1 >> |
| Functional site class: | Gamma-adaptin ear interaction motif |
| Functional site description: | The Gamma-ear interaction motif interacts with gamma-ear domains of adaptor proteins involved in clathrin coated vesicles formation at internal membranes (Golgi/endosomes) |
|---|---|
| ELMs: | LIG_AP_GAE_1 |
| Description: | The ear like domains of GGA 1-3 display significant structure and sequence similarities to that of gamma-adaptins 1 and 2. This conserved domain, referred as gamma adaptin ear (GAE), functions as a platform for recruitment of accessory proteins at the TGN. It interacts with accessory proteins such as Rabaptin-5 and gamma-synergin in mammalian cells and Ent3p and Ent5p in yeast. The interaction is mediated by an acidic Phe based motif: hydrophobic contacts between the Phe residue at position 0 and hydrophobic residues at position +3 and GAE domain are the main determinants of binding and acidic residues preceding this sequence provide a stabilizing effect. The model used for ELM prediction covers most of known true positive instances. However, as recently defined using combinatorial studies, a more comprehensive consensus for GAE binding can be defined where the central Phe residue can be substituted by any aromatic residue while aromatic residues are also functional at the second point of attachment (position +3). This extended pattern is not used presently by ELM because of its massive overprediction. |
| Pattern: | [DE][DES][DEGAS]F[SGAD][DEAP][LVIMFD] (Probability: 0.0000053) |
| Present in taxons: | Caenorhabditis elegans Eukaryota Homo sapiens Mus musculus Rattus norvegicus Saccharomyces cerevisiae Schizosaccharomyces pombe |
| Interaction Domain: |
Alpha_adaptinC2 (PF02883) |
See 11 Instances for LIG_AP_GAE_1
|
| Clathrin coated vesicles (CCV) are components of endocytosis and trafficking between the Trans Golgi Network (TGN) and endosomes. During CCV formation (less well characterized for internal membranes than for endocytosis), clathrin and clathrin adaptors interact to nucleate coat assembly by binding to appropriate membranes and recruit cargo molecules, clathrin and other coat associated proteins. The clathrin adaptors implicated in CCV formation at the TGN and endosomes are AP-1 and Ggas. AP-1 heterotetrameric complex contains a gamma-adaptin subunit bearing a gamma-ear at its carboxy terminus, while Ggas are monomeric adaptor proteins containing a C-terminal domain related to the ear domain of gamma-adaptin. By analogy with AP-2 (the well characterized adaptor complex involved in endocytic CCV formation) the gamma-ear domain of AP-1 and Ggas probably mediate interactions with accessory proteins during CCV formation. |
1 selected reference:
10 GO-Terms:
11 Instances for LIG_AP_GAE_1
(click table headers for sorting)