The Eukaryote Linear Motif resource for Functional Sites in Proteins
Accession:
Functional site class:
Clathrin box
Functional site description:
Clathrin boxes are found on cargo adaptor proteins. They interact with the N-terminal beta-propeller structure of Clathrin heavy chain. Beside the "classical" motif, some variants have been described for various types of adaptor proteins. The same protein can bear several subtypes of Clathrin boxes.
ELMs with same func. site: LIG_Clathr_ClatBox_1  LIG_Clathr_ClatBox_2 
ELM Description:
The classical clathrin box is a motif found on cargo adaptor proteins, in general near their C-terminus. This motif is sufficient for binding to the amino terminal beta propeller structure of Clathrin heavy chain. Variants of this "classical" consensus exist (see LIG_Clathr_ClatBox_2 for example) sometimes they coexist on the same protein.
Pattern: L[IVLMF].[IVLMF][DE]
Pattern Probability: 0.0003406
Present in taxons: Eukaryota Homo sapiens Mus musculus Rattus norvegicus Saccharomyces cerevisiae
Interaction Domain:
Clathrin_propel (PF01394) Clathrin propeller repeat (Stochiometry: 1 : 1)
PDB Structure: 1HX8
<a href="http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1HX8" title="" target="_blank"><img src="/media/pdb.ico.png"/>1HX8</a>
o See 18 Instances for LIG_Clathr_ClatBox_1
o Abstract
Clathrin is the main component of the coat that decorates the cytosolic face of carrier vesicles mediating protein and lipid transport in the endocytic and late secretory pathways. In vivo the multimerisation of the clathrin heterohexamer (known as triskelion) and the clathrin-mediated vesicular trafficking are regulated through a complex network of interactions involving clathrin and a number of accessory proteins.
Clathrin boxes are 5-residue-motifs found on cargo adaptor proteins, mostly near their C-terminus or central region. They are found in a large variety of accessory proteins involved in endocytosis and vesicular trafficking (specific adaptors like beta-arrestin and more general adaptor proteins like AP complexes). Unfolded flexible regions or hinges can display multiple copies of the motif, sometime belonging to different subtypes. This organisation is believed to ensure a complex, ajustable network of "weak" cooperative interactions, with the affinity between members defined by the number of motifs they possess. Extended peptides could also promote clathrin assembly by bridging the heavy chains of clathrin molecules engaged in an assembled coat. Clathin box motifs interact with the N-terminus of Clathrin heavy chain, with the so called beta-propeller structure.
o 6 selected references:

o 8 GO-Terms:

o 18 Instances for LIG_Clathr_ClatBox_1
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
Protein NameGene NameStartEndSubsequenceLogic#Ev.OrganismNotes
SMAP1_MOUSE Smap1 192 196 AAEPTIDLLGLDGPAEAPVT TP 4 Mus musculus (House mouse)
AP1B1_HUMAN AP1B1 632 636 QGDLLGDLLNLDLGPPVSGP TP 1 Homo sapiens (Human)
ARRB1_BOVIN ARRB1 376 380 ETPVDTNLIELDTNDDDIVF TP 1 Bos taurus (Cattle)
AUXI_BOVIN DNAJC6 496 500 APPEDVDLLGLEGSAVSKNF TP 2 Bos taurus (Cattle)
BIN1_HUMAN BIN1 390 394 PFSEQASLLDLDFDPLPPVT TP 3 Homo sapiens (Human)
1 
AMPH_HUMAN AMPH 351 355 EVKKEETLLDLDFDPFKPEV TP 2 Homo sapiens (Human)
2 
AP3B2_HUMAN AP3B2 808 812 PATKEISLLDLEDFTPPSVQ TP 4 Homo sapiens (Human)
ARRB2_MOUSE Arrb2 374 378 DVPVDTNLIEFDTNYATDDD TP 3 Mus musculus (House mouse)
EPN1_RAT Epn1 480 484 FLGPNAALVDLDSLVSRPGP TP 1 Rattus norvegicus (Norway rat)
AMPH_HUMAN AMPH 118 122 QKLVDGSLLTLDTYLGQFPD FP 1 Homo sapiens (Human)
BIN1_HUMAN BIN1 123 127 QKLVDQALLTMDTYLGQFPD FP 1 Homo sapiens (Human)
AP3B1_HUMAN AP3B1 818 822 PLTKDVSLLDLDDFNPVSTP TP 1 Homo sapiens (Human)
ARRB2_BOVIN ARRB2 384 388 DAPVDTNLIEFETNYATDDD TP 2 Bos taurus (Cattle)
AP180_RAT Snap91 178 182 LQGQIDALLEFDVHPNELTN FP 3 Rattus norvegicus (Norway rat)
PICA_DROME lap 192 196 LQAQLDALLEFDCQSNDLSN FP 1 Drosophila melanogaster (Fruit fly)
HIP1_HUMAN HIP1 332 336 PVLEKDDLMDMDASQQNLFD TP 4 Homo sapiens (Human)
AP2B1_BOVIN AP2B1 631 635 QGDLLGDLLNLDLGPPVNVP TP 1 Bos taurus (Cattle)
GGA2_HUMAN GGA2 349 353 GCMKTCPLIDLEVDNGPAQM TP 3 Homo sapiens (Human)
Please cite: The Eukaryotic Linear Motif Resource ELM: 10 Years and Counting (PMID:24214962)

ELM data can be downloaded & distributed for non-commercial use according to the ELM Software License Agreement
feedback@elm.eu.org