The Eukaryote Linear Motif resource for Functional Sites in Proteins
Accession:
Functional site class:
AP2 alpha ligands
Functional site description:
Motifs responsible for the binding of accessory endocytic proteins to the alpha-subunit of adaptor protein AP-2 and their recruitment to the site of clathrin coated vesicle formation.
ELMs with same func. site: LIG_AP2alpha_1  LIG_AP2alpha_2 
ELM Description:
The DPF/W motif binds the appendage of both alpha and beta subunits of AP2 adaptor complex (alpha2 and beta2 adaptin respectively). It is found on proteins involved in the control of clathrin coated vesicle (CCV) formation at sites of coated pits formation, allowing their recruitment to the site of CCV formation. Its binding site is a conserved patch on the platform subdomain of alpha2 and beta2 adaptins. Owen,2000 showed that if alpha and beta 2 adaptin share some ligands, they bind to them with different relative affinities. The difference in specificity is probably due to the different chemical properties of the residues surrounding the conserved binding site on the platform domain. For a given protein, the affinity of binding to alpha-adaptin appears to be correlated to the number of DPF/W motifs. Indeed Eps15, Epsin which contain several copies of the motif bind more tightly than amphiphysin, AP180 and auxilin which contain fewer copies (Owen,1999).
Pattern: DP[FW]
Pattern Probability: 0.0001192
Present in taxon: Eukaryota
Interaction Domain:
Alpha_adaptin_C (PF02296) Alpha adaptin AP2, C-terminal domain (Stochiometry: 1 : 1)
PDB Structure: 1KY6
<a style="white-space:nowrap;" href="http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1KY6" target="_blank"><img src="/media/pdb.ico.png"/>1KY6</a>
o See 54 Instances for LIG_AP2alpha_2
o Abstract
Vesicular traffic in the endocytic and secretory pathway is mediated by vesicles that carry cargo proteins. Clathrin-coated vesicles are responsible for a large fraction of the vescicular traffic that reaches the endosomal compartment, originating from the plasma membrane or from the TGN (trans-Golgi network). The assembly of the clathrin-coated vesicles is mediated by proteic adaptors, monomeric as AP180 and CALM or multimeric complexes like AP (Adaptor Protein) complexes. AP complexes are heterotetrameric proteins that simultaneously interact with clathrin and endocytic proteins: for example, the beta-subunit of AP-2 complex binds to clathrin, the mu-subunit interacts with Y-based sorting signal present in the cytosolic tails of membrane receptors and the alpha-subunit of AP-2 binds regulatory/accessory proteins involved in the control of clathrin coated vesicle formation.
o 3 selected references:

o 5 GO-Terms:

o 54 Instances for LIG_AP2alpha_2
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
Protein NameGene NameStartEndSubsequenceLogic#Ev.OrganismNotes
EP15R_HUMAN EPS15L1 685 687 FFKKQTKNDPFTSDPFTKNP TP 1 Homo sapiens (Human)
EPS15_HUMAN EPS15 825 827 EKDPEIFCDPFTSATTTTNK TP 1 Homo sapiens (Human)
EP15R_HUMAN EPS15L1 599 601 RGSFGAMDDPFKNKALLFSN TP 1 Homo sapiens (Human)
EP15R_HUMAN EPS15L1 624 626 HPDPFQTEDPFKSDPFKGAD TP 1 Homo sapiens (Human)
EP15R_HUMAN EPS15L1 640 642 KGADPFKGDPFQNDPFAEQQ TP 1 Homo sapiens (Human)
EP15R_HUMAN EPS15L1 656 658 AEQQTTSTDPFGGDPFKESD TP 1 Homo sapiens (Human)
EP15R_HUMAN EPS15L1 690 692 TKNDPFTSDPFTKNPSLPSK TP 1 Homo sapiens (Human)
EP15R_HUMAN EPS15L1 709 711 KLDPFESSDPFSSSSVSSKG TP 1 Homo sapiens (Human)
EP15R_HUMAN EPS15L1 728 730 GSDPFGTLDPFGSGSFNSAE TP 1 Homo sapiens (Human)
EP15R_HUMAN EPS15L1 812 814 SADFPEAPDPFQPLGADSGD TP 1 Homo sapiens (Human)
EP15R_HUMAN EPS15L1 833 835 FQSKKGFGDPFSGKDPFVPS TP 1 Homo sapiens (Human)
EPN1_RAT Epn1 349 351 AAGEGPTSDPWGSADGGAPV TP 5 Rattus norvegicus (Norway rat)
EPN1_RAT Epn1 367 369 PVSGPPSSDPWAPAPAFSDP TP 5 Rattus norvegicus (Norway rat)
EPN1_RAT Epn1 274 276 TPAPPQASDPWGGPASVPTA TP 5 Rattus norvegicus (Norway rat)
EPN1_RAT Epn1 294 296 VPVAAAASDPWGAPAVPPAA TP 5 Rattus norvegicus (Norway rat)
EPN1_RAT Epn1 306 308 APAVPPAADPWGGAAPTPAS TP 5 Rattus norvegicus (Norway rat)
AP180_RAT Snap91 400 402 VPSEAPISDPFAPEPSPPTT TP 3 Rattus norvegicus (Norway rat)
AP180_RAT Snap91 474 476 ALDACSGNDPFAPSEGSAEA TP 3 Rattus norvegicus (Norway rat)
AMPH_RAT Amph 357 359 ETLLDLDFDPFKPDVTPAGS TP 5 Rattus norvegicus (Norway rat)
AUXI_BOVIN DNAJC6 579 581 RVGEGATFDPFGAPSKPSGQ TP 3 Bos taurus (Cattle)
AUXI_BOVIN DNAJC6 605 607 LNTASASSDPFLQPTRSPSP TP 3 Bos taurus (Cattle)
AUXI_BOVIN DNAJC6 674 676 HQNKPQTLDPFADLGTLGGS TP 3 Bos taurus (Cattle)
EPN1_RAT Epn1 319 321 AAPTPASGDPWRPAAPTGPS TP 5 Rattus norvegicus (Norway rat)
EP15R_HUMAN EPS15L1 629 631 QTEDPFKSDPFKGADPFKGD TP 1 Homo sapiens (Human)
EP15R_HUMAN EPS15L1 645 647 FKGDPFQNDPFAEQQTTSTD TP 1 Homo sapiens (Human)
EP15R_HUMAN EPS15L1 661 663 TSTDPFGGDPFKESDPFRGS TP 1 Homo sapiens (Human)
EP15R_HUMAN EPS15L1 667 669 GGDPFKESDPFRGSATDDFF TP 1 Homo sapiens (Human)
EP15R_HUMAN EPS15L1 703 705 NPSLPSKLDPFESSDPFSSS TP 1 Homo sapiens (Human)
EP15R_HUMAN EPS15L1 722 724 SSVSSKGSDPFGTLDPFGSG TP 1 Homo sapiens (Human)
EP15R_HUMAN EPS15L1 768 770 LGGAGFSDDPFKSKQDTPAL TP 1 Homo sapiens (Human)
EP15R_HUMAN EPS15L1 823 825 QPLGADSGDPFQSKKGFGDP TP 1 Homo sapiens (Human)
EP15R_HUMAN EPS15L1 839 841 FGDPFSGKDPFVPSSAAKPS TP 1 Homo sapiens (Human)
EP15R_HUMAN EPS15L1 618 620 NNTQELHPDPFQTEDPFKSD TP 1 Homo sapiens (Human)
EPN1_RAT Epn1 332 334 AAPTGPSVDPWGGTPAPAAG TP 5 Rattus norvegicus (Norway rat)
EPS15_MOUSE Eps15 629 631 QSDPFVGSDPFKDDPFGKID TP 1 Mus musculus (House mouse)
EPN1_RAT Epn1 377 379 WAPAPAFSDPWGGSPAKPSS TP 6 Rattus norvegicus (Norway rat)
EPS15_HUMAN EPS15 599 601 NNRHSKEEDPFNVDSSSLTG TP 1 Homo sapiens (Human)
EPS15_HUMAN EPS15 645 647 GKIDPFGGDPFKGSDPFASD TP 1 Homo sapiens (Human)
EPS15_HUMAN EPS15 623 625 TNLDFFQSDPFVGSDPFKDD TP 1 Homo sapiens (Human)
EPS15_HUMAN EPS15 651 653 GGDPFKGSDPFASDCFFRQS TP 1 Homo sapiens (Human)
EPS15_HUMAN EPS15 672 674 DPFATSSTDPFSAANNSSIT TP 3 Homo sapiens (Human)
EPS15_HUMAN EPS15 629 631 QSDPFVGSDPFKDDPFGKID TP 1 Homo sapiens (Human)
EPS15_HUMAN EPS15 664 666 DCFFRQSTDPFATSSTDPFS TP 1 Homo sapiens (Human)
EPS15_HUMAN EPS15 692 694 SVETLKHNDPFAPGGTVVAA TP 3 Homo sapiens (Human)
EPS15_HUMAN EPS15 634 636 VGSDPFKDDPFGKIDPFGGD TP 1 Homo sapiens (Human)
EPS15_HUMAN EPS15 709 711 VAASDSATDPFASVFGNESF TP 3 Homo sapiens (Human)
EPS15_HUMAN EPS15 798 800 SINKLDSPDPFKLNDPFQPF TP 1 Homo sapiens (Human)
EPS15_HUMAN EPS15 640 642 KDDPFGKIDPFGGDPFKGSD TP 1 Homo sapiens (Human)
EPS15_HUMAN EPS15 737 739 TLSKVNNEDPFRSATSSSVS TP 3 Homo sapiens (Human)
EPS15_HUMAN EPS15 804 806 SPDPFKLNDPFQPFPGNDSP TP 1 Homo sapiens (Human)
SYNJ1_HUMAN SYNJ1 1323 1325 KRESPLKIDPFEDLSFNLLA TP 2 Homo sapiens (Human)
1 
SYNJ1_HUMAN SYNJ1 1555 1557 PPGTSPPVDPFTTLASKASP TP 2 Homo sapiens (Human)
1 
DAB2_HUMAN DAB2 293 295 NFFPTPNPDPFRDDPFTQPD TP 3 Homo sapiens (Human)
2 
DAB2_HUMAN DAB2 298 300 PNPDPFRDDPFTQPDQSTPS TP 3 Homo sapiens (Human)
2 
Please cite: The Eukaryotic Linear Motif Resource ELM: 10 Years and Counting (PMID:24214962)

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