The Eukaryote Linear Motif resource for Functional Sites in Proteins
Accession:
Functional site class:
AP2 alpha ligands
Functional site description:
Motifs responsible for the binding of accessory endocytic proteins to the alpha-subunit of adaptor protein AP-2 and their recruitment to the site of clathrin coated vesicle formation.
ELMs with same func. site: LIG_AP2alpha_1  LIG_AP2alpha_2 
ELM Description:
The DPF/W motif binds the appendage of both alpha and beta subunits of AP2 adaptor complex (alpha2 and beta2 adaptin respectively). It is found on proteins involved in the control of clathrin coated vesicle (CCV) formation at sites of coated pits formation, allowing their recruitment to the site of CCV formation. Its binding site is a conserved patch on the platform subdomain of alpha2 and beta2 adaptins. Owen,2000 showed that if alpha and beta 2 adaptin share some ligands, they bind to them with different relative affinities. The difference in specificity is probably due to the different chemical properties of the residues surrounding the conserved binding site on the platform domain. For a given protein, the affinity of binding to alpha-adaptin appears to be correlated to the number of DPF/W motifs. Indeed Eps15, Epsin which contain several copies of the motif bind more tightly than amphiphysin, AP180 and auxilin which contain fewer copies (Owen,1999).
Pattern: DP[FW]
Pattern Probability: 0.0001192
Present in taxon: Eukaryota
Interaction Domain:
Alpha_adaptin_C (PF02296) Alpha adaptin AP2, C-terminal domain (Stochiometry: 1 : 1)
PDB Structure: 1KY6
<a href="http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1KY6" title="" target="_blank"><img src="/media/pdb.ico.png"/>1KY6</a>
o See 54 Instances for LIG_AP2alpha_2
o Abstract
Vesicular traffic in the endocytic and secretory pathway is mediated by vesicles that carry cargo proteins. Clathrin-coated vesicles are responsible for a large fraction of the vescicular traffic that reaches the endosomal compartment, originating from the plasma membrane or from the TGN (trans-Golgi network). The assembly of the clathrin-coated vesicles is mediated by proteic adaptors, monomeric as AP180 and CALM or multimeric complexes like AP (Adaptor Protein) complexes. AP complexes are heterotetrameric proteins that simultaneously interact with clathrin and endocytic proteins: for example, the beta-subunit of AP-2 complex binds to clathrin, the mu-subunit interacts with Y-based sorting signal present in the cytosolic tails of membrane receptors and the alpha-subunit of AP-2 binds regulatory/accessory proteins involved in the control of clathrin coated vesicle formation.
o 3 selected references:

o 5 GO-Terms:

o 54 Instances for LIG_AP2alpha_2
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
Acc., Gene-, NameStartEndSubsequenceLogic#Ev.OrganismNotes
Q9UBC2 EPS15L1
EP15R_HUMAN
685 687 FFKKQTKNDPFTSDPFTKNP TP 1 Homo sapiens (Human)
P42566 EPS15
EPS15_HUMAN
825 827 EKDPEIFCDPFTSATTTTNK TP 1 Homo sapiens (Human)
Q9UBC2 EPS15L1
EP15R_HUMAN
599 601 RGSFGAMDDPFKNKALLFSN TP 1 Homo sapiens (Human)
Q9UBC2 EPS15L1
EP15R_HUMAN
624 626 HPDPFQTEDPFKSDPFKGAD TP 1 Homo sapiens (Human)
Q9UBC2 EPS15L1
EP15R_HUMAN
640 642 KGADPFKGDPFQNDPFAEQQ TP 1 Homo sapiens (Human)
Q9UBC2 EPS15L1
EP15R_HUMAN
656 658 AEQQTTSTDPFGGDPFKESD TP 1 Homo sapiens (Human)
Q9UBC2 EPS15L1
EP15R_HUMAN
690 692 TKNDPFTSDPFTKNPSLPSK TP 1 Homo sapiens (Human)
Q9UBC2 EPS15L1
EP15R_HUMAN
709 711 KLDPFESSDPFSSSSVSSKG TP 1 Homo sapiens (Human)
Q9UBC2 EPS15L1
EP15R_HUMAN
728 730 GSDPFGTLDPFGSGSFNSAE TP 1 Homo sapiens (Human)
Q9UBC2 EPS15L1
EP15R_HUMAN
812 814 SADFPEAPDPFQPLGADSGD TP 1 Homo sapiens (Human)
Q9UBC2 EPS15L1
EP15R_HUMAN
833 835 FQSKKGFGDPFSGKDPFVPS TP 1 Homo sapiens (Human)
O88339 Epn1
EPN1_RAT
349 351 AAGEGPTSDPWGSADGGAPV TP 5 Rattus norvegicus (Norway rat)
O88339 Epn1
EPN1_RAT
367 369 PVSGPPSSDPWAPAPAFSDP TP 5 Rattus norvegicus (Norway rat)
O88339 Epn1
EPN1_RAT
274 276 TPAPPQASDPWGGPASVPTA TP 5 Rattus norvegicus (Norway rat)
O88339 Epn1
EPN1_RAT
294 296 VPVAAAASDPWGAPAVPPAA TP 5 Rattus norvegicus (Norway rat)
O88339 Epn1
EPN1_RAT
306 308 APAVPPAADPWGGAAPTPAS TP 5 Rattus norvegicus (Norway rat)
Q05140 Snap91
AP180_RAT
400 402 VPSEAPISDPFAPEPSPPTT TP 3 Rattus norvegicus (Norway rat)
Q05140 Snap91
AP180_RAT
474 476 ALDACSGNDPFAPSEGSAEA TP 3 Rattus norvegicus (Norway rat)
O08838 Amph
AMPH_RAT
357 359 ETLLDLDFDPFKPDVTPAGS TP 5 Rattus norvegicus (Norway rat)
Q27974 DNAJC6
AUXI_BOVIN
579 581 RVGEGATFDPFGAPSKPSGQ TP 3 Bos taurus (Cattle)
Q27974 DNAJC6
AUXI_BOVIN
605 607 LNTASASSDPFLQPTRSPSP TP 3 Bos taurus (Cattle)
Q27974 DNAJC6
AUXI_BOVIN
674 676 HQNKPQTLDPFADLGTLGGS TP 3 Bos taurus (Cattle)
O88339 Epn1
EPN1_RAT
319 321 AAPTPASGDPWRPAAPTGPS TP 5 Rattus norvegicus (Norway rat)
Q9UBC2 EPS15L1
EP15R_HUMAN
629 631 QTEDPFKSDPFKGADPFKGD TP 1 Homo sapiens (Human)
Q9UBC2 EPS15L1
EP15R_HUMAN
645 647 FKGDPFQNDPFAEQQTTSTD TP 1 Homo sapiens (Human)
Q9UBC2 EPS15L1
EP15R_HUMAN
661 663 TSTDPFGGDPFKESDPFRGS TP 1 Homo sapiens (Human)
Q9UBC2 EPS15L1
EP15R_HUMAN
667 669 GGDPFKESDPFRGSATDDFF TP 1 Homo sapiens (Human)
Q9UBC2 EPS15L1
EP15R_HUMAN
703 705 NPSLPSKLDPFESSDPFSSS TP 1 Homo sapiens (Human)
Q9UBC2 EPS15L1
EP15R_HUMAN
722 724 SSVSSKGSDPFGTLDPFGSG TP 1 Homo sapiens (Human)
Q9UBC2 EPS15L1
EP15R_HUMAN
768 770 LGGAGFSDDPFKSKQDTPAL TP 1 Homo sapiens (Human)
Q9UBC2 EPS15L1
EP15R_HUMAN
823 825 QPLGADSGDPFQSKKGFGDP TP 1 Homo sapiens (Human)
Q9UBC2 EPS15L1
EP15R_HUMAN
839 841 FGDPFSGKDPFVPSSAAKPS TP 1 Homo sapiens (Human)
Q9UBC2 EPS15L1
EP15R_HUMAN
618 620 NNTQELHPDPFQTEDPFKSD TP 1 Homo sapiens (Human)
O88339 Epn1
EPN1_RAT
332 334 AAPTGPSVDPWGGTPAPAAG TP 5 Rattus norvegicus (Norway rat)
P42567 Eps15
EPS15_MOUSE
629 631 QSDPFVGSDPFKDDPFGKID TP 1 Mus musculus (House mouse)
O88339 Epn1
EPN1_RAT
377 379 WAPAPAFSDPWGGSPAKPSS TP 6 Rattus norvegicus (Norway rat)
P42566 EPS15
EPS15_HUMAN
599 601 NNRHSKEEDPFNVDSSSLTG TP 1 Homo sapiens (Human)
P42566 EPS15
EPS15_HUMAN
645 647 GKIDPFGGDPFKGSDPFASD TP 1 Homo sapiens (Human)
P42566 EPS15
EPS15_HUMAN
623 625 TNLDFFQSDPFVGSDPFKDD TP 1 Homo sapiens (Human)
P42566 EPS15
EPS15_HUMAN
651 653 GGDPFKGSDPFASDCFFRQS TP 1 Homo sapiens (Human)
P42566 EPS15
EPS15_HUMAN
672 674 DPFATSSTDPFSAANNSSIT TP 3 Homo sapiens (Human)
P42566 EPS15
EPS15_HUMAN
629 631 QSDPFVGSDPFKDDPFGKID TP 1 Homo sapiens (Human)
P42566 EPS15
EPS15_HUMAN
664 666 DCFFRQSTDPFATSSTDPFS TP 1 Homo sapiens (Human)
P42566 EPS15
EPS15_HUMAN
692 694 SVETLKHNDPFAPGGTVVAA TP 3 Homo sapiens (Human)
P42566 EPS15
EPS15_HUMAN
634 636 VGSDPFKDDPFGKIDPFGGD TP 1 Homo sapiens (Human)
P42566 EPS15
EPS15_HUMAN
709 711 VAASDSATDPFASVFGNESF TP 3 Homo sapiens (Human)
P42566 EPS15
EPS15_HUMAN
798 800 SINKLDSPDPFKLNDPFQPF TP 1 Homo sapiens (Human)
P42566 EPS15
EPS15_HUMAN
640 642 KDDPFGKIDPFGGDPFKGSD TP 1 Homo sapiens (Human)
P42566 EPS15
EPS15_HUMAN
737 739 TLSKVNNEDPFRSATSSSVS TP 3 Homo sapiens (Human)
P42566 EPS15
EPS15_HUMAN
804 806 SPDPFKLNDPFQPFPGNDSP TP 1 Homo sapiens (Human)
O43426 SYNJ1
SYNJ1_HUMAN
1323 1325 KRESPLKIDPFEDLSFNLLA TP 2 Homo sapiens (Human)
1 
O43426 SYNJ1
SYNJ1_HUMAN
1555 1557 PPGTSPPVDPFTTLASKASP TP 2 Homo sapiens (Human)
1 
P98082 DAB2
DAB2_HUMAN
293 295 NFFPTPNPDPFRDDPFTQPD TP 3 Homo sapiens (Human)
2 
P98082 DAB2
DAB2_HUMAN
298 300 PNPDPFRDDPFTQPDQSTPS TP 3 Homo sapiens (Human)
2 
Please cite: The Eukaryotic Linear Motif Resource ELM: 10 Years and Counting (PMID:24214962)

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