LIG_eIF4E_2
Accession: | |
---|---|
Functional site class: | eIF4E binding motif |
Functional site description: | Variant YxxxxL motifs that mediate binding to the dorsal surface of eIF4E through interactions with the Tryptophan at position 73 (in human and mouse). eIF4E is a key regulator of eukaryotic cap-dependent translation and these motifs play a role in both translation initiation, via interactions with eIF4G, and repression, via binding of eIF4E inhibitory proteins. |
ELMs with same func. site: | LIG_eIF4E_1 LIG_eIF4E_2 |
ELM Description: | This is a variant of the standard LIG_eIF4E_1 binding motif. Most of the instances of this motif are predictions based on a SLiMSearch of Y.PP.[ILMV]R against the human proteome. Given deviations from the consensus motif in other LIG_eIF4E_1 instances, the LIG_eIF4E_2 instance with experimental support (DDX3) could be considered an atypical LIG_eIF4E_1 instance. |
Pattern: | Y.PP.[ILMV]R |
Pattern Probability: | 0.0000013 |
Present in taxon: | Eukaryota |
Interaction Domain: |
IF4E (PF01652)
Eukaryotic initiation factor 4E
(Stochiometry: 1 : 1)
|
Abstract |
The eukaryotic translation initiation factor eIF4E recognises the 5' 7-methylguanosine cap structure of mRNA, m7GpppX, during cap-dependent translation initiation. It has been identified as the rate-limiting component of the eukaryotic translation apparatus. eIF4E directly binds to the cap structure and additional scaffolding proteins of the eIF4F complex, which recruits the 40S subunit of the ribosome. The main scaffold protein, eIF4G, binds the dorsal surface of eIF4E through a conserved motif, recruting the ribosome through additional interactions with the ribosome-associated factor eIF3. The other subunit of the eIF4F complex is eIF4A, an RNA helicase responsible for unwinding mRNA secondary structure. Due to its critical role in translation initation, eIF4E is the target of a group of "eIF4E inhibitory proteins", which competitively bind to the same region as eIF4G, sequestering the available eIF4E and preventing eIF4F complex formation. These inhibitory proteins play a key role in important cellular processes, including cell cycle progression and metabolism. Downstream activity of the Akt and mTOR signalling pathways has implicated eIF4E and its regulation in tumorigenesis and drug resistance in murine lymphoma models. Signaling via eIF4e has also been associated with altered aging phenotypes in the nematode C. elegans. |
-
Candidate tumor suppressor DDX3 RNA helicase specifically represses
cap-dependent translation by acting as an eIF4E inhibitory protein.
Shih JW, Tsai TY, Chao CH, Wu Lee YH
Oncogene 2008 Jan 24; 27 (5), 700-14
PMID: 17667941
4 GO-Terms:
5 Instances for LIG_eIF4E_2
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
Acc., Gene-, Name | Start | End | Subsequence | Logic | #Ev. | Organism | Notes |
---|---|---|---|---|---|---|---|
O75821 EIF3G EIF3G_HUMAN |
213 | 219 | QNKTGKYVPPSLRDGASRRG | TP | 13 | Homo sapiens (Human) | |
Q5C9Z4 NOM1 NOM1_HUMAN |
338 | 344 | CGSGEKYIPPHVRQAEETVD | TP | 13 | Homo sapiens (Human) | |
O15523 DDX3Y DDX3Y_HUMAN |
38 | 44 | TASKGRYIPPHLRNREASKG | TP | 13 | Homo sapiens (Human) | |
Q9BY44 EIF2A EIF2A_HUMAN |
446 | 452 | PKVATAYRPPALRNKPITNS | TP | 13 | Homo sapiens (Human) | |
O00571 DDX3X DDX3X_HUMAN |
38 | 44 | TASKGRYIPPHLRNREATKG | TP | 1 | Homo sapiens (Human) |
Please cite:
ELM-the Eukaryotic Linear Motif resource-2024 update.
(PMID:37962385)
ELM data can be downloaded & distributed for non-commercial use according to the ELM Software License Agreement
ELM data can be downloaded & distributed for non-commercial use according to the ELM Software License Agreement