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|Functional site class:||HCF-1 binding motif|
|Functional site description:||The Host Cell Factor-1 (HCF-1) is a transcriptional co-activator involved in a number of important gene regulatory complexes. HCF-1 is a chromatin associated protein and it interacts with a wide variety of cellular factors including co-activators and co-repressors that function in cell growth and cell division. It is conserved from human to nematodes. HCF-1 contains several domains that mediate specific interaction with different binding partners. In particular the N-terminal region contains six kelch repeats that fold into a six-bladed propeller domain. This propeller region is sufficient to target a number of interacting proteins by recognizing a conserved DHxY peptide sequence known as the HCF-1 binding motif (HBM).|
|Description:||The HBM is a short well-conserved sequence motif matching [ED]HxY. (A reported exception is an NHNY in BAP1 but, as this is an isolated outlier, it has not been included in the current ELM regular expression.) The motif is typically found in transcriptional regulators that affect cell cycle. HBM belongs to the common category of beta propeller-binding motifs. Although there is not yet a solved structure for the HBM in complex with the kelch repeat propeller, the evidence for the interaction is strong and the motif description appears convincing, based on strong motif conservation in multiple protein families.|
|Pattern:||[DE]H.Y (Probability: 0.0000507)|
|Present in taxons:||Metazoa|
HCF-1, the metazoan host cell factor-1 is a conserved cellular transcription factor also called VCAF1, C1, or CCF. This protein became of interest as an accessory protein required for the lytic mode of herpes simplex virus infection in association with the virion protein VP16 (reviewed by Wysocka and Herr, 2003; Kristie et al. 2010). HCF-1 is exclusively nuclear and expressed in almost all mammalian cell types (Ajuh et al., 2002). The homologues of HCF-1 are present both in vertebrates and invertebrates but with sufficient divergence that the lineages may have somewhat different functions. The association of HCF-1 with several transcription factors showed the possible role for the protein in gene transcription (Lu et al., 1997, 1998; Lu and Misra, 2000). In addition HCF-1 may play an important role in spliceosome assembly and pre-mRNA splicing in mammals (Ajuh et al., 2002), cell proliferation (Freiman and Herr, 1997; Dutta et al., 2009) and cell cycle progression (Wysocka et al., 2001). Interactions with several E2F factors are strong indications of the importance of HCF-1 for cell cycle regulation (Tyagi et al., 2007).
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