ELM - Detail for LIG

Accession:	ELME000116
Functional site class:	GYF ligand
Functional site description:	Proline-rich sequences recognized by GYF domains - involved in lymphoid signaling
ELM Description:	LIG_GYF is a proline-rich sequence specifically recognized by GYF domains. Known cases so far involve the CD2 binding protein CD2BP2, which specifically recognizes the cytoplasmic tail of CD2 surface receptor. The LIG_GYF sequence is generally composed of a pair of similar proline-rich sequences and has been shown to compete in vitro with the SH3 domain of the src-family FYN kinase. Each proline-rich sequence is spanned by positively charged residues that have complementary binding partners on the GYF domain. Due to the limited experimental data available, the current pattern is certainly overdetermined and a better description should be possible in future.
Pattern:	`[QHR].{0,1}P[PL]PP[GS]H[RH]`
Pattern Probability:	`1.686e-08`
Present in taxons:	Eukaryota Homo sapiens Mus musculus Rattus norvegicus
Interaction Domain:	GYF (PF02213) GYF domain (Stochiometry: 1 : 1) PDB Structure: 1L2Z

GYF ligand

Proline-rich sequences recognized by GYF domains - involved in lymphoid signaling

LIG_GYF is a proline-rich sequence specifically recognized by GYF domains. Known cases so far involve the CD2 binding protein CD2BP2, which specifically recognizes the cytoplasmic tail of CD2 surface receptor. The LIG_GYF sequence is generally composed of a pair of similar proline-rich sequences and has been shown to compete in vitro with the SH3 domain of the src-family FYN kinase. Each proline-rich sequence is spanned by positively charged residues that have complementary binding partners on the GYF domain. Due to the limited experimental data available, the current pattern is certainly overdetermined and a better description should be possible in future.

Pattern:

[QHR].{0,1}P[PL]PP[GS]H[RH]

Pattern Probability:

1.686e-08

Present in taxons:

Eukaryota Homo sapiens Mus musculus Rattus norvegicus

Interaction Domain:

GYF (PF02213) GYF domain (Stochiometry: 1 : 1)

PDB Structure: 1L2Z

The glycine-tyrosine-phenylalanine, or GYF, domain has been identified at the N-term of a number of proteins in organisms ranging from S.pombe to H.sapiens with fairly conserved sequence. It interacts with proline-rich sequences and, in humans, it has been shown to recognize specifically two PPPPGHR repeats at the C-term of the CD2 cell surface receptor. Despite functioning as a proline-rich peptide binding domain, the GYF fold is structurally unrelated to the SH3 or WW domains.
The NMR structure of the GYF domain of the CD2BP2 protein has been solved in complex with a proline-rich peptide (1L2Z) and the residues of the domain involved in binding have been identified. The binding pocket is very hydrophobic with a relatively smooth, concave surface. The GYF tripeptide (conserved at the C term of the domain sequence) is involved in the binding.

Biological Process:
Signal Transduction (also annotated in these classes: )
Cellular Compartment:
Cytosol (also annotated in these classes: )
Molecular Function:
Receptor (2090) (also annotated in class: )

Acc., Gene-, Name	Start	End	Subsequence	Logic	#Ev.	Organism	Notes
P06729 CD2 CD2_HUMAN	295	303	SQAPSHRPPPPGHRVQHQPQ	TP	4	Homo sapiens (Human)	1 1
P06729 CD2 CD2_HUMAN	281	289	PATSQHPPPPPGHRSQAPSH	TP	2	Homo sapiens (Human)	1L2Z 1L2Z
P37998 CD2 CD2_HORSE	276	284	NPAASQPPPPPSHRPQAPGH	TP	1	Equus caballus (Horse)

Acc., Gene-, Name

Start

End

Subsequence

Logic

#Ev.

Organism

Notes

P06729 CD2
CD2_HUMAN

295

303

SQAPSHRPPPPGHRVQHQPQ