The Eukaryotic Linear Motif resource for
Functional Sites in Proteins
Functional site class:
MAD2 binding motif
Functional site description:
Mitotic spindle checkpoint protein MAD2 binding motif
ELM Description:
Mitotic spindle checkpoint protein MAD2 binding motif
Pattern: [KR][IV][LV].....P
Pattern Probability: 0.0001011
Present in taxons: Drosophila melanogaster Eukaryota Homo sapiens Mus musculus Rattus norvegicus Saccharomyces cerevisiae Xenopus laevis
Interaction Domain:
HORMA (PF02301) HORMA domain (Stochiometry: 1 : 1)
PDB Structure: 1GO4
o See 6 Instances for LIG_MAD2
o Abstract
The spindle assembly checkpoint monitors sister chromatid bi-orientation in mitosis and prevents loss of sister chromatid cohesion in the presence of a demaged spindle. Mad2 and other proteins, such as Mad1 and Bub1, associate with kinetochores in prophase and prometaphase to monitor attachment of sister chromatids to the spindle.
The target of the spindle checkpoint is the anaphase-promoting complex (APC), a multi-subunit ubiquitin ligase, whose activity determines loss of sister chromatid cohesion and metaphase-anaphase transition. APC inhibition requires a direct interaction of Mad2 with Cdc20, a positive regulator of the APC. Mad2 also interacts with Mad1.
Cdc20 and Mad1 complexes with Mad2 are exclusive. The Mad2 binding sites on Mad1 and Cdc20 have been mapped using genetic and biochemical approaches. It has been shown that these unrelated proteins share an 10-residues Mad2 binding motif. This suggests that Mad2 is transferred from one ligand to the other via competition.
o 3 selected references:

o 4 GO-Terms:

o 6 Instances for LIG_MAD2
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
Acc., Gene-, NameStartEndSubsequenceLogic#Ev.OrganismNotes
Q12834 CDC20
129 137 DVEEAKILRLSGKPQNAPEG TP 3 Homo sapiens (Human)
541 549 DQSRTKVLHMSLNPTSVARQ TP 1 Homo sapiens (Human)
O93289 Fizzy1
137 145 DMEEAKILRLGGRPQNAPEG TP 0 Xenopus laevis (African clawed frog)
P26309 CDC20
201 209 LDMNKRILQYMPEPPKCSSL TP 0 Saccharomyces cerevisiae (Baker"s yeast)
P40957 MAD1
581 589 KEKKIRILQLRDGPFIKDQF TP 0 Saccharomyces cerevisiae (Baker"s yeast)
Q24044 fzy
176 184 HNNPLKVVYSIKTPISTKSG TP 0 Drosophila melanogaster (Fruit fly)
Please cite: The Eukaryotic Linear Motif resource: 2022 release. (PMID:34718738)

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