 << LIG_PAM2_1 << |
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| Functional site class: | PCNA binding PIP box |
| Functional site description: | The PCNA binding PIP box motif is found in proteins involved in DNA replication, repair, methylation and cell cycle control. |
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| ELMs: | LIG_PCNA_PIPBox_1 |
| Description: | The classical PCNA binding PIP Box has a core amphipathic helix which docks by the hydrophobic face into a pocket on PCNA. The first helical turn provides an aliphatic residue, often Leucine. The second helical turn provides two hydrophobic positions, being most often Phenylalanine but almost always aromatic. Preceding the PIP box helix, an amide functional group is typically present either as the protein N-terminus, or as a Glutamine residue. Following the core PIP box helix, there are no required amino acid residues. However, there are usually additional interactions that contribute to the binding affinity, for example beta augmentation backbone interactions. Many PIP boxes have positively charged residues after the core helix and, for the PIP box degrons that also bind the Cdt2 ubiquitin ligase (LIG_CRL4_Cdt2_1), multiple positive charges are always present. |
| Pattern: | ((^.{0,3})|(Q)).[^FHWY][ILM][^P][^FHILVWYP][HFM][FMY].. (Probability: 0.0000023) |
| Present in taxons: |
 cellular organisms
Eukaryota
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PDB Structure:  1U7B 
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| Interaction Domain: |
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See 18 Instances for LIG_PCNA_PIPBox_1
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| The PCNA binding site is a linear motif that mediates interaction with PCNA (Proliferating Cell Nuclear Antigen), the "sliding clamp" that associates proteins with the DNA replication fork. Among such proteins are enzymes involved in DNA replication, DNA repair and DNA methylation. Some proteins involved in cell cycle control also have PCNA binding sites. The site forms a short alpha helix which interacts with a patch on the outer surface of the clamp. The classical PCNA binding motif is termed the PIP Box. A variant PIP degron motif not only interacts with PCNA but also binds the CRL4-Cdt2 ubiquitin ligase for degradation after DNA damage or during S-phase. The PIP Box is remarkable in that it is one of the few linear motifs found in all kingdoms of life: Fen1 has a C-terminal PIP box-like motif in Eubacteria and Archaea as well as in Eukaryotes. |
2 selected references:
7 GO-Terms:
18 Instances for LIG_PCNA_PIPBox_1
(click table headers for sorting)
Please cite: ELM - the database of eukaryotic linear motifs (PMID:22110040)
ELM data can be downloaded and distributed for non-commercial use according to the ELM Software License Agreement