ELM Details - LIG_HP1

LIG_HP1_1

Functional site class:	HP1 ligand
Functional site description:	Motif in proteins that interact with the chromoshadow domain of Heterochromatin-binding protein 1.
ELM(s):	LIG_HP1_1
LIG_HP1_1 description:	Motif is a ligand to an interface formed by dimerization of two chromoshadow domains in HP1 proteins.
Pattern:	P[MVLIRWY]V[MVLIAS][LM]
Present in taxon(s):	Eukaryota
Not represented in taxon(s):

See instances for LIG_HP1_1

Abstract

HP1 is a nonhistone chromosomal protein primarily associated with heterochromatin. In mammals three variants exist: HP1 alpha, HP1 beta and HP1 gamma. All have an N-terminal chromodomain (SMART:SM00298) and a C-terminal chromoshadow domain (SMART:SM00300). The HP1 chromodomain can bind to metylated lysine 9 on histone H3, while the chromoshadow domain can mediate homo-dimerisation and interaction with other proteins. The PxVxL motif was identified as a HP1-binding motif by Smothers and Henikoff (2000 - PMID: 10660299). Several chromatin modifying proteins have been shown to interact with HP1 using this motif. Structural analysis has shown that the motif interacts with a chomoshadow domain dimer (PMID: 10747027).

Selected references

Brasher SV, Smith BO, Fogh RH, Nietlispach D, Thiru A, Nielsen PR, Broadhurst RW, Ball LJ, Murzina NV, Laue ED

The structure of mouse HP1 suggests a unique mode of single peptide recognition by the shadow chromo domain dimer.

EMBO J 2000 Apr 3;19(7) : 1587-97.

PMID: 10747027

Nielsen AL, Ortiz JA, You J, Oulad-Abdelghani M, Khechumian R, Gansmuller A, Chambon P, Losson R

Interaction with members of the heterochromatin protein 1 (HP1) family and histone deacetylation are differentially involved in transcriptional silencing by members of the TIF1 family.

EMBO J 1999 Nov 15;18(22) : 6385-95.

PMID: 10562550

Singh PB, Georgatos SD

HP1: Facts, open questions, and speculation.

J Struct Biol 2002 Oct;140(1) : 10-6.

PMID: 12490149

Smothers JF, Henikoff S

The HP1 chromo shadow domain binds a consensus peptide pentamer.

Curr Biol 2000 Jan 13;10(1) : 27-30.

PMID: 10660299

This ELM has been assigned the following Gene Ontology (GO) terms for biological process, cellular component and molecular function.

Biological Process

  DNA replication

GO:0006260

  establishment of heterochromatin silencing

GO:0006343

Cellular Component

  nucleus

GO:0005634

  heterochromatin

GO:0000792

Molecular Function

  transcription regulator

GO:0045182

Instances for LIG_HP1_1

Sequence	Position	Subsequence (Click for evidence information)	PDB	Gene Name	Protein Description	Organism
CAF1A_HUMAN	220-224	FKGKVPMVVLQDILAV	-	Name=CHAF1A; Synonyms=CAF, CAF1P150;	Chromatin assembly factor 1 subunit A (CAF-1 subunit A) (Chromatin assembly factor I p150 subunit) (CAF-I 150 kDa subunit) (CAF-Ip150).	Homo sapiens (Human).
KU70_HUMAN	351-355	LMGFKPLVLLKKHHYL	-	Name=XRCC6; Synonyms=G22P1;	ATP-dependent DNA helicase 2 subunit 1 (ATP-dependent DNA helicase II 70 kDa subunit) (Lupus Ku autoantigen protein p70) (Ku70) (70 kDa subunit of Ku antigen) (Thyroid-lupus autoantigen) (TLAA) (CTC box- binding factor 75 kDa subunit) (CTCBF) (CTC75) (DNA-repair protein XRCC6).	Homo sapiens (Human).
Q9VI63_DROME	1051-1055	PATTSPLVVLSNQLPL	-	Name=Alh; Synonyms=Alhambra; ORFNames=CG1070, Dmel_CG1070;	CG1070-PA, isoform A (AF10).	Drosophila melanogaster (Fruit fly).
TIF1A_HUMAN	713-717	STHKVPVVMLEPIRIK	-	Name=TRIM24; Synonyms=RNF82, TIF1, TIF1A;	Transcription intermediary factor 1-alpha (TIF1-alpha) (Tripartite motif-containing protein 24) (RING finger protein 82).	Homo sapiens (Human).
TIF1B_HUMAN	486-490	LMRKVPRVSLERLDLD	-	Name=TRIM28; Synonyms=KAP1, RNF96, TIF1B;	Transcription intermediary factor 1-beta (TIF1-beta) (Tripartite motif-containing protein 28) (Nuclear corepressor KAP-1) (KRAB- associated protein 1) (KAP-1) (KRAB-interacting protein 1) (KRIP-1) (RING finger protein 96).	Homo sapiens (Human).
TAF4_HUMAN	762-766	TLTQTPMVALRQPHNR	-	Name=TAF4; Synonyms=TAF2C, TAF2C1, TAF4A, TAFII130, TAFII135;	Transcription initiation factor TFIID subunit 4 (TBP-associated factor 4) (Transcription initiation factor TFIID 135 kDa subunit) (TAF(II)135) (TAFII-135) (TAFII135) (TAFII-130) (TAFII130) (RNA polymerase II TBP-associated factor subunit C).	Homo sapiens (Human).
SUV37_DROME	313-317	DAQDTPVVMMNGDVDS	-	Name=Su(var)3-7; Synonyms=Suvar(3)7; ORFNames=CG8599;	Protein suppressor of variegation 3-7.	Drosophila melanogaster (Fruit fly).
CAF1A_MOUSE	222-226	FIEKVPVVVLEDILAT	1S4Z	Name=Chaf1a; Synonyms=Caip150;	Chromatin assembly factor 1 subunit A (CAF-1 subunit A) (Chromatin assembly factor I p150 subunit) (CAF-I 150 kDa subunit) (CAF-Ip150).	Mus musculus (Mouse).