The Eukaryotic Linear Motif resource for
Functional Sites in Proteins
Accession:
Functional site class:
C-Mannosylation site
Functional site description:
Site for attachment of alpha-mannopyranosyl residue to the indole C2 carbon atom of a tryptophan residue.
ELM Description:
Motif for attachment of a mannosyl residue to a tryptophan
Pattern: (W)..W
Pattern Probability: 0.0000469
Present in taxons: Amphibia Aves Bilateria Caenorhabditis elegans Mammalia Vertebrata
Not represented in taxons: Escherichia coli Insecta Saccharomyces cerevisiae
Interaction Domain:
Glycos_transf_2 (PF00535) Glycosyl transferase family 2 (Stochiometry: 1 : 1)
o See 24 Instances for MOD_CMANNOS
o Abstract
C-Mannosylation is a type of protein glycosylation that involves covalent attachment of an alpha-mannopyranosyl residue to the indole C2 carbon atom of tryptophan via a C-C link (Hofsteenge,1994; de Beer,1995). The exact recognition sequence was determined by site-directed mutagenesis of individual amino acids and was found to be WXXW, where the first tryptophan residue becomes C-mannosylated. The significance of the amino acids in both X positions was not studied as the shortest peptides used consisted of only four amino acids forming a recognition sequence, WAKW (Hartmann,2000). The search for the pattern, restricted to the mammalian proteins that cross the endoplasmic reticulum (ER) membrane, yielded 336 proteins. Some of the proteins found in the database search have already been examined for the presence of C-mannosylation. In total, 49 C-mannosylated tryptophan residues were found in 11 proteins. The precursor in the biosynthesis of (C2-Man)-Trp is dolichylphosphate mannose (Dol-P-Man) precursor in the biosynthetic pathway of C-mannosyltryptophan (Doucey,1998). The whole biosynthetic pathway, from GDPMan through Dol-P-Man to the C-mannosylated peptide was reconstructed in vitro. The activity was found in Caenorhabditis elegans, Amphibia, Aves, Mammalia, but not in Escherichia coli, Insecta and Saccharomyces cerevisiae (Doucey,1998; Krieg,1997). C-mannosyltransferase activity can be found in most of the parts of the mammalian organism (Doucey,1998).
o 1 selected reference:

o 1 GO-Term:

o 24 Instances for MOD_CMANNOS
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
Acc., Gene-, NameStartEndSubsequenceLogic#Ev.OrganismNotes
P02748 C9
CO9_HUMAN
48 51 HIDCRMSPWSEWSQCDPCLR TP 1 Homo sapiens (Human)
P07358 C8B
CO8B_HUMAN
548 551 KNTPIDGKWNCWSNWSSCSG TN 1 Homo sapiens (Human)
P07358 C8B
CO8B_HUMAN
551 554 PIDGKWNCWSNWSSCSGRRK TP 1 Homo sapiens (Human)
P07358 C8B
CO8B_HUMAN
70 73 PIDCELSSWSSWTTCDPCQK TP 1 Homo sapiens (Human)
P07357 C8A
CO8A_HUMAN
545 548 KADGSWSCWSSWSVCRAGIQ TP 1 Homo sapiens (Human)
P07357 C8A
CO8A_HUMAN
542 545 EGAKADGSWSCWSSWSVCRA TP 1 Homo sapiens (Human)
P07357 C8A
CO8A_HUMAN
44 47 AVTCQLSNWSEWTDCFPCQD TP 1 Homo sapiens (Human)
P10643 C7
CO7_HUMAN
506 509 GVDGGWSCWSSWSPCVQGKK TP 1 Homo sapiens (Human)
P13671 C6
CO6_HUMAN
571 574 AVDGQWGCWSSWSTCDATYK TP 1 Homo sapiens (Human)
P13671 C6
CO6_HUMAN
568 571 KSNAVDGQWGCWSSWSTCDA TP 1 Homo sapiens (Human)
P13671 C6
CO6_HUMAN
29 32 ACFCDHYAWTQWTSCSKTCN TP 1 Homo sapiens (Human)
P27918 CFP
PROP_HUMAN
318 321 VPCPVDGEWDSWGEWSPCIR TN 1 Homo sapiens (Human)
P27918 CFP
PROP_HUMAN
80 83 CQPCRSPRWSLWSTWAPCSV TN 1 Homo sapiens (Human)
P27918 CFP
PROP_HUMAN
385 388 PLKGSWSEWSTWGLCMPPCG TP 1 Homo sapiens (Human)
P27918 CFP
PROP_HUMAN
382 385 QHCPLKGSWSEWSTWGLCMP TP 1 Homo sapiens (Human)
P27918 CFP
PROP_HUMAN
321 324 PVDGEWDSWGEWSPCIRRNM TP 1 Homo sapiens (Human)
P27918 CFP
PROP_HUMAN
260 263 PPCPVAGGWGPWGPVSPCPV TP 1 Homo sapiens (Human)
P27918 CFP
PROP_HUMAN
199 202 PTHGAWATWGPWTPCSASCH TP 1 Homo sapiens (Human)
P27918 CFP
PROP_HUMAN
196 199 QVCPTHGAWATWGPWTPCSA TP 1 Homo sapiens (Human)
P27918 CFP
PROP_HUMAN
142 145 PEMGGWSGWGPWEPCSVTCS TP 1 Homo sapiens (Human)
P27918 CFP
PROP_HUMAN
139 142 QCCPEMGGWSGWGPWEPCSV TP 1 Homo sapiens (Human)
P27918 CFP
PROP_HUMAN
83 86 CRSPRWSLWSTWAPCSVTCS TP 1 Homo sapiens (Human)
P29460 IL12B
IL12B_HUMAN
319 322 RAQDRYYSSSWSEWASVPCS TP 1 Homo sapiens (Human)
P10153 RNASE2
RNAS2_HUMAN
34 37 HVKPPQFTWAQWFETQHINM TP 1 Homo sapiens (Human)
Please cite: ELM 2016-data update and new functionality of the eukaryotic linear motif resource. (PMID:26615199)

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