ELM - instance LIG_SH3_2 in sequence P50570 at position 829

Instance

Accession	Acc. Gene-, Name	Start	End	Subsequence	Logic	PDB	Organism	Length
ELMI002131	P50570 DNM2 DYN2_HUMAN	829	834	SDLFPAPPQIPSRPVRIPPG	TP	---	Homo sapiens (Human)	870

Instance evidence

Evidence class	PSI-MI	Method	BioSource	PubMed	Logic	Reliability	Notes
experimental	MI:0096	pull down	in vivo/in vitro		support	certain	InteractionDetection
experimental	MI:0519	glutathione s tranferase tag	in vitro	Kojima,2004	support	certain
experimental	MI:0077	nuclear magnetic resonance	in vitro	Kojima,2004	support	certain	InteractionDetection FeatureDetection
experimental	MI:0405	competition binding	in vitro	Kojima,2004	support	certain	InteractionDetection
experimental	MI:0107	surface plasmon resonance	in vitro	Kojima,2004	support	certain	InteractionDetection FeatureDetection

Interactions

Uniprot Id	Domain family	Domain Start	Domain End	Affinity Min/Max (µMol)	Notes
(O00499) BIN1_HUMAN	PF07653 (SH3_2) Variant SH3 domain	524	590		[mitab][xml]

Switches

This ELM instance is part of the following 1 switching mechanism annotated at the switches.ELM resource:

SWTI000328:

An intramolecular interaction of a Bin1 SH3 binding motif, encoded by exon 10, with the Dynamin-2 (DNM2) SH3 domain prevents binding of dynamin2 to the Bin1 SH3 domain. Binding of PI(4,5)P2 to the overlapping PI(4,5)P2 binding motif encoded by exon 10 relieves the intramolecular auto-inhibitory interaction and allows the Bin1 SH3 domain to interact with the dynamin2 PxxP motif

Pathways

KEGG: The sequence P50570 is implicated in the following 7 Pathways: (color codes: This sequence=red, interacting sequence=orange)

Please cite: ELM-the Eukaryotic Linear Motif resource-2024 update. (PMID:37962385)

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