The Eukaryotic Linear Motif resource for
Functional Sites in Proteins

o  Instance

Accession Acc. Gene-, NameStartEndSubsequenceLogic PDB OrganismLength
ELMI002262 O15350 TP73
P73_HUMAN
484 487 VSGSHCTPPPPYHADPSLVS TP --- Homo sapiens (Human) 636

o  Instance evidence

Evidence classPSI-MIMethodBioSourcePubMedLogicReliabilityNotes
experimental MI:0096 pull down in vivo/in vitro support certain InteractionDetection
experimental MI:0521 his tag in vivo/in vitro/in silico Strano,2001 support certain
experimental MI:0096 pull down in vivo/in vitro support certain InteractionDetection
experimental MI:0519 glutathione s tranferase tag in vitro Strano,2001 support certain
experimental MI:0096 pull down in vivo/in vitro support certain InteractionDetection
experimental MI:0519 glutathione s tranferase tag in vitro Rossi,2005 support certain
experimental MI:0997 ubiquitinase assay in vitro Rossi,2005 support certain InteractionDetection
experimental MI:0007 anti tag coimmunoprecipitation in vivo Rossi,2005 support certain InteractionDetection
experimental MI:0074 mutation analysis in vivo/in vitro Rossi,2005 support certain FeatureDetection
experimental MI:0019 coimmunoprecipitation in vivo/in vitro Strano,2001 support certain InteractionDetection
experimental MI:0074 mutation analysis in vivo/in vitro Strano,2001 support certain FeatureDetection

o  Interactions

Uniprot Id Domain family Domain Start Domain End Affinity Min/Max (µMol) Notes
(Q96J02) ITCH_HUMAN PF00397 (WW)
WW domain
[mitab][xml]
(P46937) YAP1_HUMAN PF00397 (WW)
WW domain
[mitab][xml]

o  Switches



This ELM instance is part of the following 1 switching mechanism annotated at the switches.ELM resource:
  • SWTI000678:
    ELM Switch SWTI000678

    The transcriptional coactivator YAP1 and the ubiquitin ligase Itch competitively bind to the same WW-binding motif of p73. Binding of YAP1 prevents Itch-mediated ubiquitylation of p73, resulting in stabilisation, and increases trancriptional activity of p73.

o  Pathways

KEGG: The sequence O15350 is implicated in the following 4 Pathways: (color codes: This sequence=red, interacting sequence=orange) Reactome: The sequence O15350 is implicated in the following 4 Pathways:
Please cite: ELM 2016-data update and new functionality of the eukaryotic linear motif resource. (PMID:26615199)

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