ELM - instance LIG_WW_1 in sequence O15350 at position 484

Instance

Accession	Acc. Gene-, Name	Start	End	Subsequence	Logic	PDB	Organism	Length
ELMI002262	O15350 TP73 P73_HUMAN	484	487	VSGSHCTPPPPYHADPSLVS	TP	---	Homo sapiens (Human)	636

Instance evidence

Evidence class	PSI-MI	Method	BioSource	PubMed	Logic	Reliability	Notes
experimental	MI:0096	pull down	in vivo/in vitro		support	certain	InteractionDetection
experimental	MI:0521	his tag	in vivo/in vitro/in silico	Strano,2001	support	certain
experimental	MI:0096	pull down	in vivo/in vitro		support	certain	InteractionDetection
experimental	MI:0519	glutathione s tranferase tag	in vitro	Strano,2001	support	certain
experimental	MI:0096	pull down	in vivo/in vitro		support	certain	InteractionDetection
experimental	MI:0519	glutathione s tranferase tag	in vitro	Rossi,2005	support	certain
experimental	MI:0997	ubiquitinase assay	in vitro	Rossi,2005	support	certain	InteractionDetection
experimental	MI:0007	anti tag coimmunoprecipitation	in vivo	Rossi,2005	support	certain	InteractionDetection
experimental	MI:0074	mutation analysis	in vivo/in vitro	Rossi,2005	support	certain	FeatureDetection
experimental	MI:0019	coimmunoprecipitation	in vivo/in vitro	Strano,2001	support	certain	InteractionDetection
experimental	MI:0074	mutation analysis	in vivo/in vitro	Strano,2001	support	certain	FeatureDetection

Interactions

Uniprot Id	Domain family	Domain Start	Domain End	Affinity Min/Max (µMol)	Notes
(Q96J02) ITCH_HUMAN	PF00397 (WW) WW domain				[mitab][xml]
(P46937) YAP1_HUMAN	PF00397 (WW) WW domain				[mitab][xml]

Switches

This ELM instance is part of the following 1 switching mechanism annotated at the switches.ELM resource:

SWTI000678:

The transcriptional coactivator YAP1 and the ubiquitin ligase Itch competitively bind to the same WW-binding motif of p73. Binding of YAP1 prevents Itch-mediated ubiquitylation of p73, resulting in stabilisation, and increases trancriptional activity of p73.

Pathways

KEGG: The sequence O15350 is implicated in the following 4 Pathways: (color codes: This sequence=red, interacting sequence=orange)

Please cite: ELM-the Eukaryotic Linear Motif resource-2024 update. (PMID:37962385)

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