ELM - instance LIG_PDZ_Class_1 in sequence P22756-2 at position 900

Instance

Accession	Acc. Gene-, Name	Start	End	Subsequence	Logic	PDB	Organism	Length
ELMI002591	P22756-2 Grik1 GRIK1_RAT	900	905	SFTSILTCHQRRTQRKETVA	TP	---	Rattus norvegicus (Norway rat)	905

Instance evidence

Evidence class	PSI-MI	Method	BioSource	PubMed	Logic	Reliability	Notes
experimental	MI:0096	pull down	in vivo/in vitro		support	certain	InteractionDetection
experimental	MI:0519	glutathione s tranferase tag	in vitro	Hirbec,2003	support	certain
experimental	MI:0018	two hybrid	in vivo	Hirbec,2003	support	certain	InteractionDetection
experimental	MI:0405	competition binding	in vitro	Hirbec,2003	support	certain	InteractionDetection
experimental	MI:0074	mutation analysis	in vivo/in vitro	Hirbec,2003	support	certain	FeatureDetection

Interactions

Uniprot Id	Domain family	Domain Start	Domain End	Affinity Min/Max (µMol)	Notes
(Q9EP80) PICK1_RAT	PF00595 (PDZ) PDZ domain (Also known as DHR or GLGF)	22	101	101.0/101.0	[mitab][xml]

Switches

This ELM instance is part of the following 1 switching mechanism annotated at the switches.ELM resource:

SWTI000560:

Alternative splicing removes the PDZ-binding motif of Glutamate receptor, ionotropic kainate 1 (Grik1), abrogating binding to PRKCA-binding protein (Pick1). The ER-retention motif of Grik1 splice variants can be inhibited by PKC phosphorylation and association with a PDZ protein. It has also been shown that the PDZ domain-containing proteins Disks large homolog 4 (Dlg4) and Syntenin-1 (Sdcbp) are able to bind.

Please cite: ELM-the Eukaryotic Linear Motif resource-2024 update. (PMID:37962385)

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