ELM - instance LIG_Actin_RPEL_3 in sequence Q8VIM5 at position 64

Instance

Accession	Acc. Gene-, Name	Start	End	Subsequence	Logic	PDB	Organism	Length
ELMI001671	Q8VIM5 Myocd MYCD_MOUSE	64	83	LRRKGRNRSDRASLVTMHIL	TP	---	Mus musculus (House mouse)	935

Instance evidence

Evidence class	PSI-MI	Method	BioSource	PubMed	Logic	Reliability	Notes
experimental	MI:0096	pull down	in vivo/in vitro		support	certain	InteractionDetection
experimental	MI:0519	glutathione s tranferase tag	in vitro	Guettler,2008	support	certain
experimental	MI:0074	mutation analysis	in vivo/in vitro	Guettler,2008	support	certain	FeatureDetection
experimental	MI:0053	fluorescence polarization spectroscopy	in vitro	Guettler,2008	support	certain	InteractionDetection
experimental	MI:0367	green fluorescent protein tag	in vivo	Guettler,2008	support	certain
experimental	MI:0005	alanine scanning	in vivo/in vitro/in silico	Guettler,2008	support	certain	FeatureDetection

Interactions

Uniprot Id	Domain family	Domain Start	Domain End	Affinity Min/Max (µMol)	Notes
(P68135) ACTS_RABIT	PF00022 (Actin) Actin	4	377		[mitab][xml]

Switches

This ELM instance is part of the following 1 switching mechanism annotated at the switches.ELM resource:

SWTI000512:

Alternative promoter usage removes the RPEL-binding motif of Myocardin (Myocd), abrogating binding to Actin, alpha skeletal muscle (Acta1). 3 RPEL motifs are found in Myocd. The shortened form lacks 2 RPEL motifs but no definitive comparison of binding to actin has been undertaken between isoforms.

Please cite: ELM-the Eukaryotic Linear Motif resource-2024 update. (PMID:37962385)

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