ELM - instance LIG_Actin_RPEL_3 in sequence Q8K4J6 at position 105

Instance

Accession	Acc. Gene-, Name	Start	End	Subsequence	Logic	PDB	Organism	Length
ELMI001666	Q8K4J6 Mkl1 MKL1_MOUSE	105	124	LNEKIAQRPGPMELVEKNIL	TP	2YJE 2YJF	Mus musculus (House mouse)	964

Instance evidence

Evidence class	PSI-MI	Method	BioSource	PubMed	Logic	Reliability	Notes
experimental	MI:0096	pull down	in vivo/in vitro		support	certain	InteractionDetection
experimental	MI:0519	glutathione s tranferase tag	in vitro	Vartiainen,2007	support	certain
experimental	MI:0096	pull down	in vivo/in vitro		support	certain	InteractionDetection
experimental	MI:0519	glutathione s tranferase tag	in vitro	Guettler,2008	support	certain
experimental	MI:0074	mutation analysis	in vivo/in vitro	Guettler,2008	support	certain	FeatureDetection
experimental	MI:0053	fluorescence polarization spectroscopy	in vitro	Guettler,2008	support	certain	InteractionDetection
experimental	MI:0367	green fluorescent protein tag	in vivo	Guettler,2008	support	certain
experimental	MI:0005	alanine scanning	in vivo/in vitro/in silico	Guettler,2008	support	certain	FeatureDetection
experimental	MI:0105	structure based prediction	in silico	Mouilleron,2011	support	certain	InteractionDetection
experimental	MI:0074	mutation analysis	in vivo/in vitro	Mouilleron,2011	support	certain	FeatureDetection
experimental	MI:0071	molecular sieving	in vivo/in vitro	Mouilleron,2011	support	certain	InteractionDetection
experimental	MI:0016	circular dichroism	in vitro	Mouilleron,2011	support	certain	InteractionDetection
experimental	MI:0005	alanine scanning	in vivo/in vitro/in silico	Mouilleron,2011	support	certain	FeatureDetection
experimental	MI:0071	molecular sieving	in vivo/in vitro	Vartiainen,2007	support	certain	InteractionDetection
experimental	MI:0005	alanine scanning	in vivo/in vitro/in silico	Vartiainen,2007	support	certain	FeatureDetection
experimental	MI:0074	mutation analysis	in vivo/in vitro	Vartiainen,2007	support	certain	FeatureDetection
experimental	MI:0074	mutation analysis	in vivo/in vitro	Miralles,2003	support	certain	FeatureDetection
experimental	MI:0019	coimmunoprecipitation	in vivo/in vitro	Miralles,2003	support	certain	InteractionDetection
experimental	MI:0403	colocalization	in vitro	Miralles,2003	support	certain
experimental	MI:0101	sequence based prediction	in silico	Miralles,2003	support	certain	InteractionDetection
experimental	MI:0074	mutation analysis	in vivo/in vitro	Mouilleron,2008	support	certain	FeatureDetection
experimental	MI:0053	fluorescence polarization spectroscopy	in vitro	Mouilleron,2008	support	certain	InteractionDetection
experimental	MI:0005	alanine scanning	in vivo/in vitro/in silico	Mouilleron,2008	support	certain	FeatureDetection

Interactions

Uniprot Id	Domain family	Domain Start	Domain End	Affinity Min/Max (µMol)	Notes
(P68135) ACTS_RABIT	PF00022 (Actin) Actin	4	377	17.8/19.8	[mitab][xml]

Switches

This ELM instance is part of the following 1 switching mechanism annotated at the switches.ELM resource:

SWTI000318:

Hiding of the NLS of MKL/myocardin-like protein 1 (Mkl1) by binding of G-actin to the RPEL motifs of MKL/myocardin-like protein 1 (Mkl1) prevents translocation of this transcription factor to the nucleus.

Please cite: ELM-the Eukaryotic Linear Motif resource-2024 update. (PMID:37962385)

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