ELM - instance DOC_AGCK_PIF_1 in sequence Q13464 at position 394

Instance

Accession	Acc. Gene-, Name	Start	End	Subsequence	Logic	PDB	Organism	Length
ELMI001627	Q13464 ROCK1 ROCK1_HUMAN	394	399	FVGNQLPFVGFTYYSNRRYL	TP	---	Homo sapiens (Human)	1354

Instance evidence

Evidence class	PSI-MI	Method	BioSource	PubMed	Logic	Reliability	Notes
experimental	MI:0096	pull down	in vivo/in vitro		support	likely	InteractionDetection
experimental	MI:0519	glutathione s tranferase tag	in vitro	Pinner,2008	support	likely
experimental	MI:0074	mutation analysis	in vivo/in vitro	Pinner,2008	support	likely	FeatureDetection

Interactions

Uniprot Id	Domain family	Domain Start	Domain End	Affinity Min/Max (µMol)	Notes
(O15530) PDPK1_HUMAN	PF00069 (Pkinase) Protein kinase domain	82	341		[mitab][xml]

Switches

This ELM instance is part of the following 1 switching mechanism annotated at the switches.ELM resource:

SWTI000154:

Phosphorylation of T398 in the PIF pocket-binding motif of Rho-associated protein kinase 1 (ROCK1) induces intramolecular binding and kinase cis-activation.

Pathways

KEGG: The sequence Q13464 is implicated in the following 20 Pathways: (color codes: This sequence=red, interacting sequence=orange)

Please cite: ELM-the Eukaryotic Linear Motif resource-2024 update. (PMID:37962385)

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