ELM - instance LIG_PDZ_Class_1 in sequence P35222 at position 776

Instance

Accession	Acc. Gene-, Name	Start	End	Subsequence	Logic	PDB	Organism	Length
ELMI001576	P35222 CTNNB1 CTNB1_HUMAN	776	781	LMDGLPPGDSNQLAWFDTDL	TP	3DIW	Homo sapiens (Human)	781

Instance evidence

Evidence class	PSI-MI	Method	BioSource	PubMed	Logic	Reliability	Notes
experimental	MI:0065	isothermal titration calorimetry	in vitro	Zhang,2008	support	certain	InteractionDetection
experimental	MI:0114	x-ray crystallography	in vitro	Zhang,2008	support	certain	InteractionDetection FeatureDetection

Interactions

Uniprot Id	Domain family	Domain Start	Domain End	Affinity Min/Max (µMol)	Notes
(Q9DBG9) TX1B3_MOUSE	PF00595 (PDZ) PDZ domain (Also known as DHR or GLGF)	17	110	0.14/0.24	[mitab][xml]

Switches

This ELM instance is part of the following 2 switching mechanisms annotated at the switches.ELM resource:

SWTI000218:

Binding of Ezrin (EZR) via its FERM domain to the EB domain of Na(+)/H(+) exchange regulatory cofactor NHE-RF1 (SLC9A3R1) results in allosteric coupling to the second PDZ domain of Na(+)/H(+) exchange regulatory cofactor NHE-RF1 (SLC9A3R1), which results in relief of the intramolecular interaction with the PDZ binding ligand, thereby increasing the affinity of the PDZ domain for other ligands, including Catenin beta-1 (CTNNB1).
SWTI000333:

Binding of Ezrin via its FERM domain to the EB domain of Na(+)/H(+) exchange regulatory cofactor NHE-RF1 (SLC9A3R1) results in allosteric coupling to the second PDZ domain of SLC9A3R1. This relieves the intramolecular interaction with the SLC9A3R1 PDZ-binding ligand and increases the affinity of the PDZ domain for other ligands including Catenin beta-1 (CTNNB1).

Pathways

KEGG: The sequence P35222 is implicated in the following 30 Pathways: (color codes: This sequence=red, interacting sequence=orange)

Please cite: The Eukaryotic Linear Motif resource: 2022 release. (PMID:34718738)

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