ELM - instance LIG_PTB_Apo_2 in sequence P05067 at position 756

Instance

Accession	Acc. Gene-, Name	Start	End	Subsequence	Logic	PDB	Organism	Length
ELMI001555	P05067 APP A4_HUMAN	756	763	SKMQQNGYENPTYKFFEQMQ	TP	1AQC 1X11 3DXC 3DXD 3DXE	Homo sapiens (Human)	770

Instance evidence

Evidence class	PSI-MI	Method	BioSource	PubMed	Logic	Reliability	Notes
experimental	MI:0096	pull down	in vivo/in vitro		support	certain	InteractionDetection
experimental	MI:0519	glutathione s tranferase tag	in vitro	Howell,1999	support	certain
experimental	MI:0074	mutation analysis	in vivo/in vitro	Howell,1999	support	certain	FeatureDetection
experimental	MI:0018	two hybrid	in vivo	Howell,1999	support	certain	InteractionDetection
experimental	MI:0107	surface plasmon resonance	in vitro	Zhang,1997	support	certain	InteractionDetection FeatureDetection
experimental	MI:0114	x-ray crystallography	in vitro	Zhang,1997	support	certain	InteractionDetection FeatureDetection
experimental	MI:0065	isothermal titration calorimetry	in vitro	Radzimanowski,2008	support	certain	InteractionDetection
experimental	MI:0114	x-ray crystallography	in vitro	Radzimanowski,2008	support	certain	InteractionDetection FeatureDetection
experimental	MI:0005	alanine scanning	in vivo/in vitro/in silico	Borg,1996	support	certain	FeatureDetection

Interactions

Uniprot Id	Domain family	Domain Start	Domain End	Affinity Min/Max (µMol)	Notes
(Q02410) APBA1_HUMAN	PF00640 (PID) Phosphotyrosine interaction domain (PTB/PID)	461	618	0.32/0.32	[mitab][xml]
(O00213) APBB1_HUMAN	PF00640 (PID) Phosphotyrosine interaction domain (PTB/PID)			0.2/0.2	[mitab][xml]
(O75553) DAB1_HUMAN	PF00640 (PID) Phosphotyrosine interaction domain (PTB/PID)	42	168	0.55/0.55	[mitab][xml]

Switches

This ELM instance is part of the following 2 switching mechanisms annotated at the switches.ELM resource:

SWTI000201:

Phosphorylation of T743 adjacent to the PTB-binding motif of Amyloid beta A4 protein (APP) reduces the affinity for Amyloid beta A4 precursor protein-binding family B member 1 (APBB1).
SWTI000292:

Phosphorylation of Y757 in APP (Amyloid beta A4 protein (APP)) switches its specificity from PTB domain containing proteins, like Amyloid beta A4 precursor protein-binding family B member 1 (APBB1), which is involved in trafficking and processing of APP, to SH2 domain containing proteins, such as Growth factor receptor-bound protein 2 (GRB2).

Pathways

KEGG: The sequence P05067 is implicated in the following 3 Pathways: (color codes: This sequence=red, interacting sequence=orange)

Please cite: ELM-the Eukaryotic Linear Motif resource-2024 update. (PMID:37962385)

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