ELM - instance TRG_NLS_MonoExtN_4 in sequence P38398 at position 501

Instance

Accession	Acc. Gene-, Name	Start	End	Subsequence	Logic	PDB	Organism	Length
ELMI001375	P38398 BRCA1 BRCA1_HUMAN	501	508	ERPLTNKLKRKRRPTSGLHP	TP	---	Homo sapiens (Human)	1863

Instance evidence

Evidence class	PSI-MI	Method	BioSource	PubMed	Logic	Reliability	Notes
experimental	MI:0074	mutation analysis	in vivo/in vitro	Chen,1996	support	certain	FeatureDetection
experimental	MI:0403	colocalization	in vitro	Chen,1996	support	certain
experimental	MI:0018	two hybrid	in vivo	Chen,1996	support	certain	InteractionDetection

Switches

This ELM instance is part of the following 1 switching mechanism annotated at the switches.ELM resource:

SWTI000580:

Alternative splicing removes the nuclear localisation signal (NLS) of Breast cancer type 1 susceptibility protein (BRCA1), abrogating binding to Importin subunit alpha-1 (KPNA1) and import into the nucleus. The study compared the full-length Brca1 splice variant (Isoform 1) to the Delta11b isoform (Isoform Delta11b). The shorter isoform is missing exon 11b and differs in a number of ways. Firstly, it lacks an NLS and therefore has a cytoplasmic localisation. Also, when over-expressed, the Delta11b isoform was not toxic, suggesting nuclear localisation is important for Brca1\'s toxic behaviour.

Pathways

KEGG: The sequence P38398 is implicated in the following 7 Pathways: (color codes: This sequence=red, interacting sequence=orange)

Please cite: The Eukaryotic Linear Motif resource: 2022 release. (PMID:34718738)

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