ELM - instance LIG_PDZ_Class_1 in sequence P97288-2 at position 382

Instance

Accession	Acc. Gene-, Name	Start	End	Subsequence	Logic	PDB	Organism	Length
ELMI002429	P97288-2 Htr4 5HT4R_MOUSE	382	387	HQELEKLPIHNDPESLESCF	TP	---	Mus musculus (House mouse)	387

Instance evidence

Evidence class	PSI-MI	Method	BioSource	PubMed	Logic	Reliability	Notes
experimental	MI:0004	affinity chromatography technology	in vivo/in vitro	Joubert,2004	support	certain	InteractionDetection
experimental	MI:0007	anti tag coimmunoprecipitation	in vivo	Joubert,2004	support	certain	InteractionDetection
experimental	MI:0663	confocal microscopy	in vivo/in vitro	Joubert,2004	support	certain	InteractionDetection
experimental	MI:0074	mutation analysis	in vivo/in vitro	Joubert,2004	support	certain	FeatureDetection

Interactions

Uniprot Id	Domain family	Domain Start	Domain End	Affinity Min/Max (µMol)	Notes
(Q3UHD6) SNX27_MOUSE	PF00595 (PDZ) PDZ domain (Also known as DHR or GLGF)	41	131		[mitab][xml]

Switches

This ELM instance is part of the following 3 switching mechanisms annotated at the switches.ELM resource:

SWTI000497:

Alternative splicing removes the PDZ-binding motif of Isoform 5-HT4(A) of 5-hydroxytryptamine receptor 4 (Htr4), abrogating binding to Sorting nexin-27 (Snx27). Snx27 is responsible for targeting of the Isoform 5-HT4(A) to early endosomes.
SWTI000498:

Alternative splicing removes the PDZ-binding motif of Isoform 5-HT4(A) of 5-hydroxytryptamine receptor 4 (Htr4), abrogating binding to Na(+)/H(+) exchange regulatory cofactor NHE-RF1 (Slc9a3r1). Isoform 5-HT4(A) interacts specifically with a protein complex including Slc9a3r1 and Ezrin (Ezr) that might participate in its targeting to specialised subcellular regions, such as microvilli.
SWTI000655:

Alternative splicing removes the PDZ-binding motif of Isoform 5-HT4(A) of 5-hydroxytryptamine receptor 4 (Htr4), abrogating binding to Na(+)/H(+) exchange regulatory cofactor NHE-RF1 (Slc9a3r1). Isoform 5-HT4(A) interacts specifically with a protein complex including Slc9a3r1 and Ezrin (Ezr) that might participate in its targeting to specialised subcellular regions, such as microvilli.

Please cite: ELM-the Eukaryotic Linear Motif resource-2024 update. (PMID:37962385)

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