| Accession | Acc. Gene-, Name | Start | End | Subsequence | Logic | PDB | Organism | Length |
|---|---|---|---|---|---|---|---|---|
| ELMI002120 | P13569 CFTR CFTR_HUMAN |
1475 | 1480 | KPQIAALKEETEEEVQDTRL | TP |
1I92 
|
Homo sapiens (Human) | 1480 |
Instance evidence
| Evidence class | PSI-MI | Method | BioSource | PubMed | Logic | Reliability | Notes |
|---|---|---|---|---|---|---|---|
| experimental | MI:0096 | pull down | in vivo/in vitro | support | certain | InteractionDetection | |
| experimental | MI:0519 | glutathione s tranferase tag | in vitro | Short,1998 | support | certain | |
| experimental | MI:0114 | x-ray crystallography | in vitro | Karthikeyan,2001 | support | certain | InteractionDetection FeatureDetection |
| experimental | MI:0047 | far western blotting | in vitro | Short,1998 | support | certain | InteractionDetection |
| experimental | MI:0107 | surface plasmon resonance | in vitro | Short,1998 | support | certain | InteractionDetection FeatureDetection |
| experimental | MI:0074 | mutation analysis | in vivo/in vitro | Short,1998 | support | certain | FeatureDetection |
| experimental | MI:0113 | western blot | in vitro | Short,1998 | support | certain | ParticipantIdentification FeatureDetection |
Interactions
| Uniprot Id | Domain family | Domain Start | Domain End | Affinity Min/Max (µMol) | Notes |
|---|---|---|---|---|---|
| (O14745) NHRF1_HUMAN | PF00595
(PDZ)
PDZ domain (Also known as DHR or GLGF) |
[mitab][xml] |
Switches
This ELM instance is part of the following 1 switching mechanism annotated at the switches.ELM resource:
-
SWTI000342:
The PDZ domains of Na(+)/H(+) exchange regulatory cofactor NHE-RF1 (SLC9A3R1) and SH3 and multiple ankyrin repeat domains protein 2 (SHANK2) compete for the PDZ-binding motif of Cystic fibrosis transmembrane conductance regulator (CFTR). SLC9A3R1 positively regulates CFTR activity by recruiting a PKA-containing complex, while SH3 and multiple ankyrin repeat domains protein 2 (SHANK2) negatively affects CFTR activity by recruiting PDE4D.
Pathways
Please cite:
ELM-the Eukaryotic Linear Motif resource-2024 update.
(PMID:37962385)
ELM data can be downloaded & distributed for non-commercial use according to the ELM Software License Agreement
ELM data can be downloaded & distributed for non-commercial use according to the ELM Software License Agreement