ELM - instance LIG_PDZ_Class_1 in sequence O55207-3 at position 1288

Instance

Accession	Acc. Gene-, Name	Start	End	Subsequence	Logic	PDB	Organism	Length
ELMI002583	O55207-3 Synj2 SYNJ2_RAT	1288	1293	FVRTVAAQRLTPVDASGSSV	TP	---	Rattus norvegicus (Norway rat)	1293

Instance evidence

Evidence class	PSI-MI	Method	BioSource	PubMed	Logic	Reliability	Notes
experimental	MI:0096	pull down	in vivo/in vitro		support	certain	InteractionDetection
experimental	MI:0519	glutathione s tranferase tag	in vitro	Nemoto,1999	support	certain
experimental	MI:0411	elisa	in vitro	Nemoto,1999	support	certain	InteractionDetection ParticipantIdentification
experimental	MI:0004	affinity chromatography technology	in vivo/in vitro	Nemoto,1999	support	certain	InteractionDetection
experimental	MI:0047	far western blotting	in vitro	Nemoto,1999	support	certain	InteractionDetection
experimental	MI:0018	two hybrid	in vivo	Nemoto,1999	support	certain	InteractionDetection
experimental	MI:0074	mutation analysis	in vivo/in vitro	Nemoto,1999	support	certain	FeatureDetection
experimental	MI:0018	two hybrid	in vivo	Nemoto,2001	support	certain	InteractionDetection
experimental	MI:0074	mutation analysis	in vivo/in vitro	Nemoto,2001	support	certain	FeatureDetection

Interactions

Uniprot Id	Domain family	Domain Start	Domain End	Affinity Min/Max (µMol)	Notes
(Q9WVJ4) SYJ2B_RAT	PF00595 (PDZ) PDZ domain (Also known as DHR or GLGF)	13	97		[mitab][xml]

Switches

This ELM instance is part of the following 1 switching mechanism annotated at the switches.ELM resource:

SWTI000590:

Alternative splicing removes the PDZ-binding motif of Isoform 7.5kb of Synaptojanin-2 (Synj2), abrogating binding to Synaptojanin-2-binding protein (Synj2bp). Isoform 7.5kb (Synaptojanin 2A) is recruited to mitochondria through the interaction with the PDZ domain of the mitochondrial outer membrane protein Synj2bp.

Please cite: The Eukaryotic Linear Motif resource: 2022 release. (PMID:34718738)

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