The Eukaryotic Linear Motif resource for
Functional Sites in Proteins

o  Instance

Accession Acc. Gene-, NameStartEndSubsequenceLogic PDB OrganismLength
ELMI003266 O60566 BUB1B
BUB1B_HUMAN
528 533 SKGPSVPFSIFDEFLLSEKK TP --- Homo sapiens (Human) 1050

o  Instance evidence

Evidence classPSI-MIMethodBioSourcePubMedLogicReliabilityNotes
experimental MI:0096 pull down in vivo/in vitro support likely InteractionDetection
experimental MI:0519 glutathione s tranferase tag in vitro Diaz-Martinez,2015 support likely
experimental MI:1247 microscale thermophoresis in vitro Diaz-Martinez,2015 support likely InteractionDetection
experimental MI:0074 mutation analysis in vivo/in vitro Diaz-Martinez,2015 support likely FeatureDetection
experimental MI:0997 ubiquitinase assay in vitro Diaz-Martinez,2015 support likely InteractionDetection
experimental MI:0019 coimmunoprecipitation in vivo/in vitro Diaz-Martinez,2015 support likely InteractionDetection
experimental MI:0096 pull down in vivo/in vitro Di Fiore,2015 support likely InteractionDetection
experimental MI:0074 mutation analysis in vivo/in vitro Di Fiore,2015 support likely FeatureDetection
experimental MI:0405 competition binding in vitro Di Fiore,2015 support likely InteractionDetection

o  Interactions

Uniprot Id Domain family Domain Start Domain End Affinity Min/Max (µMol) Notes
(Q12834) CDC20_HUMAN PF00400 (WD40)
WD domain, G-beta repeat
1.2/1.2 [mitab][xml]

o  Pathways

The sequence O60566 is implicated in the following 2 Pathways: (color codes: This sequence=red, interacting sequence=orange)
Please cite: ELM 2016-data update and new functionality of the eukaryotic linear motif resource. (PMID:26615199)

ELM data can be downloaded & distributed for non-commercial use according to the ELM Software License Agreement
feedback@elm.eu.org