| Accession | Acc. Gene-, Name | Start | End | Subsequence | Logic | PDB | Organism | Length |
|---|---|---|---|---|---|---|---|---|
| ELMI001991 | P84022 SMAD3 SMAD3_HUMAN |
176 | 181 | GIEPQSNIPETPPPGYLSED | TP |
2LB3 
|
Homo sapiens (Human) | 425 |
Instance evidence
| Evidence class | PSI-MI | Method | BioSource | PubMed | Logic | Reliability | Notes |
|---|---|---|---|---|---|---|---|
| experimental | MI:0096 | pull down | in vivo/in vitro | support | certain | InteractionDetection | |
| experimental | MI:0519 | glutathione s tranferase tag | in vitro | Nakano,2009 | support | certain | |
| experimental | MI:0077 | nuclear magnetic resonance | in vitro | Aragon,2011 | support | certain | InteractionDetection FeatureDetection |
| experimental | MI:0019 | coimmunoprecipitation | in vivo/in vitro | Nakano,2009 | support | certain | InteractionDetection |
| experimental | MI:0005 | alanine scanning | in vivo/in vitro/in silico | Nakano,2009 | support | certain | FeatureDetection |
Switches
This ELM instance is part of the following 2 switching mechanisms annotated at the switches.ELM resource:
-
SWTI000068:
Phosphorylation of T179 in the Pin1-binding motif of Mothers against decapentaplegic homolog 3 (SMAD3) induces binding to the Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (PIN1) protein.
-
SWTI000279:
CDK8/9 phosphorylates Mothers against decapentaplegic homolog 3 (SMAD3) at T179 and S208. Phosphorylation of T179 creates a binding site for the WW domain of Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (PIN1), while phosphorylation of S208 primes SMAD3 for phosphorylation of S204 by Glycogen synthase kinase-3 beta (GSK3B). The pS204-pS208 forms a binding site for the third WW domain of E3 ubiquitin-protein ligase NEDD4-like (NEDD4L), whose second WW domain will displace the WW domain of PIN1 at the pT179-PY box site of SMAD3. This regulation couples SMAD3 activation to SMAD3 destruction in an ordered fashion. See also switch details and switch details.
Pathways
- AGE RAGE signaling pathway in diabetic complications
- Adherens junction
- Apelin signaling pathway
- Cell cycle
- Cellular senescence
- Chronic myeloid leukemia
- Colorectal cancer
- Diabetic cardiomyopathy
- Endocytosis
- FoxO signaling pathway
- Gastric cancer
- Hepatitis B
- Hepatocellular carcinoma
- Hippo signaling pathway
- Human T cell leukemia virus 1 infection
- Inflammatory bowel disease
- Pancreatic cancer
- Pathways in cancer
- Signaling pathways regulating pluripotency of stem cells
- TGF beta signaling pathway
- Th17 cell differentiation
- Wnt signaling pathway
Please cite:
ELM-the Eukaryotic Linear Motif resource-2024 update.
(PMID:37962385)
ELM data can be downloaded & distributed for non-commercial use according to the ELM Software License Agreement
ELM data can be downloaded & distributed for non-commercial use according to the ELM Software License Agreement