ELM - instance DOC_AGCK_PIF_1 in sequence O00141 at position 418

Instance

Accession	Acc. Gene-, Name	Start	End	Subsequence	Logic	PDB	Organism	Length
ELMI001630	O00141 SGK1 SGK1_HUMAN	418	423	VKEAAEAFLGFSYAPPTDSF	TP	---	Homo sapiens (Human)	431

Instance evidence

Evidence class	PSI-MI	Method	BioSource	PubMed	Logic	Reliability	Notes
experimental	MI:0096	pull down	in vivo/in vitro		support	likely	InteractionDetection
experimental	MI:0519	glutathione s tranferase tag	in vitro	Biondi,2001	support	likely
experimental	MI:0424	protein kinase assay	in vivo/in vitro	Biondi,2001	support	likely	InteractionDetection
experimental	MI:0405	competition binding	in vitro	Biondi,2001	support	likely	InteractionDetection
experimental	MI:0074	mutation analysis	in vivo/in vitro	Biondi,2001	support	likely	FeatureDetection
experimental	MI:0107	surface plasmon resonance	in vitro	Biondi,2001	support	likely	InteractionDetection FeatureDetection

Interactions

Uniprot Id	Domain family	Domain Start	Domain End	Affinity Min/Max (µMol)	Notes
(O15530) PDPK1_HUMAN	PF00069 (Pkinase) Protein kinase domain	82	341		[mitab][xml]

Switches

This ELM instance is part of the following 1 switching mechanism annotated at the switches.ELM resource:

SWTI000149:

Phosphorylation of S422 by Serine/threonine-protein kinase mTOR (MTOR) (as part of mTORC2 complex) in the PIF pocket-binding motif of Serine/threonine-protein kinase Sgk1 (SGK1) induces intramolecular binding and kinase cis-activation.

Pathways

KEGG: The sequence O00141 is implicated in the following 4 Pathways: (color codes: This sequence=red, interacting sequence=orange)

Please cite: The Eukaryotic Linear Motif resource: 2022 release. (PMID:34718738)

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