The Eukaryotic Linear Motif resource for
Functional Sites in Proteins

o  Instance

Accession Acc. Gene-, NameStartEndSubsequenceLogic PDB OrganismLength
ELMI001314 P16471 PRLR
348 353 TYLDPDTDSGRGSCDSPSLL TP --- Homo sapiens (Human) 622

o  Instance evidence

Evidence classPSI-MIMethodBioSourcePubMedLogicReliabilityNotes
experimental MI:0424 protein kinase assay in vivo Li,2004 support likely InteractionDetection
experimental MI:0019 coimmunoprecipitation in vivo/in vitro Li,2004 support likely InteractionDetection

o  Switches

This ELM instance is part of the following 2 switching mechanisms annotated at the switches.ELM resource:
  • SWTI000160:
    ELM Switch SWTI000160

    Dual phosphorylation of S349 and S353 in the TrCP1-binding motif of Prolactin receptor (PRLR) targets the protein to the SCF ubiquitin ligase complex, which marks it for degradation.
  • SWTI000625:
    ELM Switch SWTI000625

    Alternative Splicing removes the degron motif of Prolactin receptor (PRLR) abrogating binding to F-box/WD repeat-containing protein 11 (FBXW11). SCF-beta-TrCP2 negatively regulates the long isoform of PRLR (Isoform 1). Should be noted that TrCP2 has a predominately cytoplasmic localisation compared to TrCP1 that is located in the nucleus. The mechanism for the down-regulation of the intermediate (Isoform Intermediate) and short (Isoform Short form 1a and Isoform Short form 1b) that lack the TrCP degron is still not known. However it should be noted that the short and intermediate are either partially deficient or entirely deficient in mediating the signal transduction pathways induced by PRL (see Kine et al. (1999) (here) and Ross et al. (1997) (here)). This though is likely due to the missing STAT5-SH2-binding motif.

o  Pathways

KEGG: The sequence P16471 is implicated in the following 5 Pathways: (color codes: This sequence=red, interacting sequence=orange)
Please cite: The Eukaryotic Linear Motif resource: 2022 release. (PMID:34718738)

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