ELM - instance DEG_SCF_TRCP1_1 in sequence P16471 at position 348

Instance

Accession	Acc. Gene-, Name	Start	End	Subsequence	Logic	PDB	Organism	Length
ELMI001314	P16471 PRLR PRLR_HUMAN	348	353	TYLDPDTDSGRGSCDSPSLL	TP	---	Homo sapiens (Human)	622

Instance evidence

Evidence class	PSI-MI	Method	BioSource	PubMed	Logic	Reliability	Notes
experimental	MI:0424	protein kinase assay	in vivo	Li,2004	support	likely	InteractionDetection
experimental	MI:0019	coimmunoprecipitation	in vivo/in vitro	Li,2004	support	likely	InteractionDetection

Switches

This ELM instance is part of the following 2 switching mechanisms annotated at the switches.ELM resource:

SWTI000160:

Dual phosphorylation of S349 and S353 in the TrCP1-binding motif of Prolactin receptor (PRLR) targets the protein to the SCF ubiquitin ligase complex, which marks it for degradation.
SWTI000625:

Alternative Splicing removes the degron motif of Prolactin receptor (PRLR) abrogating binding to F-box/WD repeat-containing protein 11 (FBXW11). SCF-beta-TrCP2 negatively regulates the long isoform of PRLR (Isoform 1). Should be noted that TrCP2 has a predominately cytoplasmic localisation compared to TrCP1 that is located in the nucleus. The mechanism for the down-regulation of the intermediate (Isoform Intermediate) and short (Isoform Short form 1a and Isoform Short form 1b) that lack the TrCP degron is still not known. However it should be noted that the short and intermediate are either partially deficient or entirely deficient in mediating the signal transduction pathways induced by PRL (see Kine et al. (1999) (here) and Ross et al. (1997) (here)). This though is likely due to the missing STAT5-SH2-binding motif.

Pathways

KEGG: The sequence P16471 is implicated in the following 5 Pathways: (color codes: This sequence=red, interacting sequence=orange)

Please cite: The Eukaryotic Linear Motif resource: 2022 release. (PMID:34718738)

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