ELM - instance LIG_SH2_SRC in sequence P97318 at position 198

Instance

Accession	Acc. Gene-, Name	Start	End	Subsequence	Logic	PDB	Organism	Length
ELMI002611	P97318 Dab1 DAB1_MOUSE	198	201	EEDVEDPVYQYIVFEAGHEP	TP	---	Mus musculus (House mouse)	588

Instance evidence

Evidence class	PSI-MI	Method	BioSource	PubMed	Logic	Reliability	Notes
experimental	MI:0047	far western blotting	in vitro	Gao,2012	support	certain	InteractionDetection
experimental	MI:0424	protein kinase assay	in vitro	Keshvara,2001	support	certain	InteractionDetection
experimental	MI:0424	protein kinase assay	in vivo	Keshvara,2001	support	certain	InteractionDetection
experimental	MI:0074	mutation analysis	in vivo/in vitro	Keshvara,2001	support	certain	FeatureDetection

Switches

This ELM instance is part of the following 2 switching mechanisms annotated at the switches.ELM resource:

SWTI000620:

Alternative splicing removes the SH2-binding motif of Disabled homolog 1 (Dab1), abrogating binding to Neuronal proto-oncogene tyrosine-protein kinase Src (Src). Splice variants 2 and 3 (only containing one of the YQxI motifs, i.e. Y185 and Y198) exhibit decreased tyrosine phosphorylation, suggesting both motifs are required for full activation of Dab1. Dab1 is likely to recruit Neuronal proto-oncogene tyrosine-protein kinase Src (Src) via these two YQxI motifs, which subsequently phosphorylates adjacent YxVP motifs (here). This was also suggested for Phosphatidylinositol 3-kinase regulatory subunit alpha (Pik3r1) and Suppressor of cytokine signaling 2 (Socs2). Gao et al. (2012) (here) suggests that the ability of different Dab1 isoforms to recruit distinct sets of SH2 domains allows a fine-tuning role for Dab1 splicing in the intricate series of events that underlie neuronal migration (See also Katyal & Godbout (2004) (here) and Gao et al. (2010) (here)).
SWTI000624:

Phosphorylation of Y198 in the SH2-binding motif of Disabled homolog 1 (Dab1) induces binding to Neuronal proto-oncogene tyrosine-protein kinase Src (Src).

Pathways

KEGG: The sequence P97318 is implicated in the following 1 Pathway: (color codes: This sequence=red, interacting sequence=orange)

Please cite: The Eukaryotic Linear Motif resource: 2022 release. (PMID:34718738)

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