The Eukaryote Linear Motif resource for Functional Sites in Proteins
Functional site class:
MAPK docking motif
Functional site description:
The docking interaction in the MAP kinase cascades is achieved through specific conserved regions on MAPKs (docking groove) and MAPK-interacting molecules (the MAPK docking motif). The docking motif is usually - but not always - the sequence of a substrate protein.
ELMs with this model: DOC_MAPK_1  DOC_MAPK_2 
Description:
MAPK interacting molecules (e.g. MAPKKs, substrates, phosphatases) carry docking motifs that help to regulate specific interactions in the MAPK cascade. The classic MAPK docking site was first identified in c-JUN, a substrate of JNK (Kallunki,1996). This motif is characterized by a cluster of basic residues positioned upstream to a #-x-# motif. The substrates that carry this motif recognize a broad range of MAPKs (Sharrocks,2000).
Pattern: [KR]{0,2}[KR].{0,2}[KR].{2,4}[ILVM].[ILVF]
Pattern Probability: 0.0043238
Present in taxon: Eukaryota
Interaction Domain:
Pkinase (PF00069) Protein kinase domain (Stochiometry: 1 : 1)
o See 16 Instances for DOC_MAPK_1
o Abstract
MAPK (Mitogen-activated protein kinase) signalling pathways are characterized by a cascade of multiple kinases that contribute to regulate a variety of extracellular stimuli, thereby to control the cellular environment. The MAPK family includes the ERKs (extracellular signal-regulated kinase), the JNKs (c-Jun N-terminal kinase), the p38 MAPKs and ERK5. The ERKs generally regulate cellular proliferation and differentiation processes in response to growth factors and other hormones while the JNKs and p38 MAPKs are primarily activated in response to extracellular stresses, such as U.V. irradiation, osmotic stress or inflammatory stimuli (Biondi,2003). MAPKs are serine/threonine kinases that tend to phosphorylate their substrates on S/T-P motif. As the sequence S/T-P is found in ~ 80% of all proteins additional factors are required to direct individual kinases towards the correct substrates (Bardwell,2006).
One way by which the MAPKs ensure their substrate specificity is by interaction through docking motifs, short amino acid stretches located on MAPK-interacting proteins. The classical docking motif is characterized by a cluster of at least two positively charged amino acids followed by by a spacer of 2-to-6 residues from a hydrophobic-X-hydrophobic sequence, where the hydrophobic residues are long-chain aliphatics (usually Leu, Ile). Both in the spacer and in the sequence immediately C-terminal to the hydrophobic-X-hydrophobic element, there is a high propensity for the presence of Pro, Asn, and/or Gly, which are residues that are both turn-forming and helix-breaking (Bardwell,2001). Another type of docking motif is a short peptide containing the sequence FXFP that is usually downstream of the phosphorylation site. This motif was originally found to bind the ERK subfamily but lately it has been shown to promote the interaction with p38alpha and p38 beta2 (Jacobs,1999; Galanis,2001). Substrates can contain the classical docking motif or the FXFP or often contain both. (Sharrocks,2000).
o 8 selected references:

o 7 GO-Terms:

o 16 Instances for DOC_MAPK_1
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
Protein NameGene NameStartEndSubsequenceLogic#Ev.OrganismNotes
STE7_YEAST STE7 9 19 KTLQRRNLKGLNLNLHPDVG TP 3 Saccharomyces cerevisiae (Baker"s yeast)
MSG5_YEAST MSG5 29 38 RSLQNRNTKNLSLDIAALHP TP 3 Saccharomyces cerevisiae (Baker"s yeast)
DUS1_HUMAN DUSP1 54 62 STIVRRRAKGAMGLEHIVPN TP 2 Homo sapiens (Human)
DUS6_HUMAN DUSP6 64 73 PGIMLRRLQKGNLPVRALFT TP 3 Homo sapiens (Human)
DAPK1_HUMAN DAPK1 1385 1393 LRELGRRDAADFLLKASSVF FP 1 Homo sapiens (Human)
ELK1_HUMAN ELK1 312 321 ISQPQKGRKPRDLELPLSPS TP 1 Homo sapiens (Human)
ELK4_HUMAN ELK4 318 327 VNNSSRSKKPKGLELAPTLV TP 2 Homo sapiens (Human)
JUN_HUMAN JUN 32 42 YSNPKILKQSMTLNLADPVG TP 2 Homo sapiens (Human)
MEF2A_HUMAN MEF2A 269 277 LGMNSRKPDLRVVIPPSSKG TP 2 Homo sapiens (Human)
MP2K4_HUMAN MAP2K4 40 48 SSMQGKRKALKLNFANPPFK TP 2 Homo sapiens (Human)
MP2K1_HUMAN MAP2K1 3 11 MPKKKPTPIQLNPAPDGSAV TP 2 Homo sapiens (Human)
STE7_YEAST STE7 62 71 SNLFLRRGIKKKLTLDAFGD TP 3 Saccharomyces cerevisiae (Baker"s yeast)
DIG1_YEAST DIG1 97 107 KKSLKRGRVPAPLNLSDSNT TP 1 Saccharomyces cerevisiae (Baker"s yeast)
DIG2_YEAST DIG2 100 110 NHSLKRKRVPPALNFSDIQA TP 1 Saccharomyces cerevisiae (Baker"s yeast)
ELK3_MOUSE Elk3 292 301 PSLPPKGKKPKGLEISAPQL TP 1 Mus musculus (House mouse)
MP2K1_HUMAN MAP2K1 4 11 MPKKKPTPIQLNPAPDGSAV TP 1 Homo sapiens (Human)
1 
Please cite: The Eukaryotic Linear Motif Resource ELM: 10 Years and Counting (PMID:24214962)

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