The Eukaryotic Linear Motif resource for
Functional Sites in Proteins
Functional site class:
Integrin binding sites
Functional site description:
Integrins are cell surface receptors responsible for cell migration, cell to extracellular matrix adhesion, and cell to cell adhesion. Integrins are composed of one alpha and one beta subunits. NGR motif is an inactive precursor in fibronectin and fibrillin-1 that undergoes deamidation and forms active isoDGR motif capable of binding RGD-binding pocket of several integrins including alphaVbeta3 and alpha5beta1 integrins. Binding of isoDGR motif to RGD-binding site in integrins inhibits endothelial cells adhesion (Curnis,2006). RGD-binding site is a composite binding site in integrins made of alpha and beta subunits. Integrins are used by viruses to gain entry into the host. Example is adeno-associated type II virus which binds to integrin alpha5beta1 via NGR motif to gain viral entry into the host cell (Asokan,2006). NGR motif-containing peptides coupled to certain drugs are specific against CD13-positive tumour angiogenic vessels.
ELMs with same func. site: LIG_IBS_1  LIG_Integrin_isoDGR_1  LIG_RGD 
ELM Description:
NGR (asparagine-glycine-arginine) is a tripeptide present in fibronectin (FINC_HUMAN), fibrillin-1 (FBN1_HUMAN), and adeno-associated virus 2 protein capsid (CAPSD_AAV2S). Asparagine deamidation of NGR motif yields isoDGR (isoaspartic acid-glycine-arginine) motif. Deamidation is a non-enzymatic process that involves formation of succinimide intermediate and subsequent formation of isoDGR by hydrolysis. The isoDGR motif is able to interact with integrins by recognition of RGD-binding site of several integrins including αVβ3 integrin. RGD composite binding site is formed by both alpha and beta subunits of integrins and is located on the extracellular side of plasma membrane. IsoDGR binds to RGD-binding site in inverted orientation compared to proteins that contain RGD motif (Spitaleri,2008). CisoDGRC is a cyclic peptide that competes with RGD-containing peptides for binding to αVβ3 integrins (Curnis,2006). CisoDGRC binding to RGD-binding pocket inhibits endothelial cell adhesion, proliferation, and tumour growth. Both ligands have similar binding affinity for αVβ3 integrin. CisoDGRC binds to the following integrins with decrease in affinity: αVβ3, α5β1, αVβ6, αVβ5, αVβ8. Linear isoDGR (GisoDGRG) binds to αVβ6, αVβ3, α5β1, αVβ5, and αVβ8. Cyclic isoDGR binds with 10-100-fold increased affinity to αVβ3 compared to other integrins. Acetylation of either linear or cyclic isoDGR increases the affinity for integrins accompanied by loss of specificity. Peptide linearization (replacing flanking glycines with cysteines) is associated with 100-fold loss of αVβ3 binding affinity and specificity. Hence, flanking residues of NGR motif affect affinity and specificity for integrin binding (Curnis,2010).
Pattern: NGR
Pattern Probability: 0.0001597
Present in taxons: Bos taurus Canis lupus familiaris Danio rerio Gallus gallus Homo sapiens Metazoa Pan troglodytes Rattus norvegicus Sus scrofa Xenopus laevis
Interaction Domains:
o See 8 Instances for LIG_Integrin_isoDGR_1
o Abstract
Integrins are transmembrane receptors responsible primarily for cell migration and extracellular matrix adhesion. Integrins are heterodimers, composed of one alpha, and one beta subunit. They function through bidirectional signalling. There are 18 alpha and 8 beta subunits in the integrin family that assemble into 24 heterodimers. alpha subunits determine integrin ligand specificity whereas beta subunits are connected to the cytoskeleton. alpha and beta subunits are held by non-covalent interactions. Integrins are able to bind a variety of ligands including cell surface adhesion proteins and extracellular matrix proteins. Integrin signalling involves assembly of receptor-ligand complexes on extracellular side of plasma membrane. Integrin proteins are present only in Metazoa with no integrins found in Fungi, Viridiplantae, or TAXON:2. The structure of integrin includes extracellular domain which contains ligand binging sites, plasma membrane regions, and short cytoplasmic domains (Barczyk,2009, Campbell,2011).
A hallmark of integrins is the ability of individual family members to recognize multiple ligands. Most integrins recognize relatively short peptide motifs such as RGD, LDV, DLXXL, NGR and, in general, a key constituent residue is an acidic amino acid. Some collagens have another - different - integrin binding motif. The ligand specificities rely on both subunits of a given α-β heterodimer. Proteins that contain RGD motifs recognise 8 out of 24 integrins. RGD was originally identified as the sequence in fibronectin that engages the fibronectin receptor, integrin α5β1. RGD sequences have also been found to be responsible for the cell adhesive properties of a number of other proteins, including fibrinogen, victronectin, von Willebrand factor and many other glycoproteins. Many snake venoms are rich in RGD peptides - a testament to the importance of the integrin system. While their motifs may be more benign, the pharmaceutical industry also finds the integrin-RGD system to be of considerable interest. Antagonists could be effective for therapeutic intervention in cancer, thrombosis and numerous inflammatory conditions.
The IsoDGR motif of integrin-binding ligands arises from the NGR motif via deamidation of asparagine (N) or from the DGR motif via isomerisation of aspartic acid (D). Deamidation is a non-enzymatic reaction that involves the formation of succinimide intermediate and its hydrolysis generating aspartic acid and isoaspartic acid (isoD) residues. The ratio of aspartic acid to isoaspartic acid residues during the deamidation process is 1:3. The D-isoDGR peptide was shown to poorly compete for the RGD binding site. Hence the interaction of isoDGR with integrin is stereospecific favouring L-isomer with L-isoaspartic acid residues being most predominant after deamidation (Curnis,2006, Corti,2011). One of the factors that control the rate of deamidation is the presence of specific amino acids near the asparagine or isoaspartic acid residues. For example glycine near these residues accelerates the process of isoDGR formation (Curnis,2010). Newly formed isoDGR motif mimics a RGD motif and recognises the RGD-binding site of integrins (Corti,2011). One of the proteins that binds to certain integrins via an isoDGR motif is fibronectin (FN). Fibronectins are proteins involved in cell adhesion, motility, shape maintenance, and healing. There are two highly conserved NGR sequences in orthologues of fibronectin. 5th and 7th FN-I repeats each contain a NGR motif that is conserved in human, rat, bird, murine, amphibian, and fish proteins. Both NGR sequences are flanked by glycine residues (GNGRG). IsoDGR motif of fibronectin interacts with integrins by recognition of RGD-binding site of αVβ3, αVβ5, αVβ6, αVβ8, and α5β1 integrins. Integrins such as α1β1, α3β1, α4β7, α5β7, α6β4, and α9β1 cannot be recognised by isoDGR-containing peptides (Curnis,2006). Full-length plasma fibronectin is resistant to asparagine deamidation compared to short fibronectin fragments or peptides containing NGR motif. Hence deamidation of NGR in fibronectin requires proteolytic cleavage. Intracellular enzyme protein-L-isoAsp-O-methyltransferase (PIMT_HUMAN) can inhibit the effects of asparagine deamidation. PIMT converts L-isoaspartic acid and D-aspartic acid to L-aspartic acid residues through methyl-esterification. PIMT can only target extracellular proteins when it is released into extracellular space by damaged vessels and injured tissues. PIMT restores the primary amino acid sequence in case of aspartic acid isomerisation but not in case of asparagine deamidation (Corti,2008, Corti,2011).
NGR and isoDGR motifs might have therapeutic applications that are currently being evaluated. IsoDGR motif-containing peptides are able to recognise αVβ3 integrin-positive endothelial cells in tumour vessels and inhibit tumour growth in tumour-bearing mice as well as inhibit endothelial cells proliferation and adhesion to vitronectin. IsoDGR peptides can be exploited as integrin antagonists for the treatment of cancer and other diseases since isoDGR competes with RGD-containing protein for the RGD-binding pocket (Corti,2011). NGR motif can recognise tumour vasculature by binding to aminopeptidase N (CD13). CD13 is a membrane-bound metalloproteinase that has been implicated in tumour angiogenesis. It is barely expressed by endothelium of normal blood vessels but is significantly upregulated in angiogenic tumour blood vessels. NGR peptide was also shown to target CD13 in inflammation and retinal disorders. Cyclic NGR (NGR flanked by single cysteine on both sides) peptide binds to CD13-positive blood vessels in tumours but not to epithelium of normal kidney or other CD13-rich tissues. First anticancer drug that was coupled to NGR peptide was doxorubicin. It showed reduced toxicity and improved efficacy against human cancer xenografts in nude mice compared to free doxorubicin. NGR peptide has also been coupled to tumour necrosis factor α (TNF-α) that has improved anti-tumour activity. This compound has underwent phase I and phase II trials with 50% of patients treated being stabilised, and having limited toxicity (Corti,2008, Corti,2011).
o 10 selected references:

o 7 GO-Terms:

o 8 Instances for LIG_Integrin_isoDGR_1
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
Acc., Gene-, NameStartEndSubsequenceLogic#Ev.OrganismNotes
P35555 FBN1
2304 2306 QTKPGICENGRCLNTRGSYT TP 4 Homo sapiens (Human)
P11276 Fn1
501 503 MMRCTCVGNGRGEWACIPYS TP 3 Mus musculus (House mouse)
P11276 Fn1
264 266 LLQCVCTGNGRGEWKCERHA TP 2 Mus musculus (House mouse)
P02751 FN1
1432 1434 YVVSIVALNGREESPLLIGQ TP 4 Homo sapiens (Human)
P02751 FN1
501 503 MMRCTCVGNGRGEWTCIAYS TP 5 Homo sapiens (Human)
P02751 FN1
367 369 PCVLPFTYNGRTFYSCTTEG TP 4 Homo sapiens (Human)
P02751 FN1
263 265 LLQCICTGNGRGEWKCERHT TP 6 Homo sapiens (Human)
P03135 Capsid protein VP1
511 513 TGATKYHLNGRDSLVNPGPA TP 6 Adeno-associated virus 2 Srivastava/1982
Please cite: The eukaryotic linear motif resource - 2018 update. (PMID:29136216)

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