The Eukaryotic Linear Motif resource for
Functional Sites in Proteins
Functional site class:
MAPK docking motif
Functional site description:
The docking interaction in the MAP kinase cascades is achieved through specific conserved regions on MAPKs (docking groove) and MAPK-interacting molecules (the MAPK docking motif). The docking motif is usually - but not always - the sequence of a substrate protein.
ELMs with same func. site: DOC_MAPK_1  DOC_MAPK_2 
ELM Description:
MAPK interacting molecules (e.g. MAPKKs, substrates, phosphatases) carry docking motifs that help to regulate specific interactions in the MAPK cascade. The classic MAPK docking site was first identified in c-JUN, a substrate of JNK (Kallunki,1996). This motif is characterized by a cluster of basic residues positioned upstream to a #-x-# motif. The substrates that carry this motif recognize a broad range of MAPKs (Sharrocks,2000).
Pattern: [KR]{0,2}[KR].{0,2}[KR].{2,4}[ILVM].[ILVF]
Pattern Probability: 0.0043238
Present in taxon: Eukaryota
Interaction Domain:
Pkinase (PF00069) Protein kinase domain (Stochiometry: 1 : 1)
o See 15 Instances for DOC_MAPK_1
o Abstract
MAPK (Mitogen-activated protein kinase) signalling pathways are characterized by a cascade of multiple kinases that contribute to regulate a variety of extracellular stimuli, thereby to control the cellular environment. The MAPK family includes the ERKs (extracellular signal-regulated kinase), the JNKs (c-Jun N-terminal kinase), the p38 MAPKs and ERK5. The ERKs generally regulate cellular proliferation and differentiation processes in response to growth factors and other hormones while the JNKs and p38 MAPKs are primarily activated in response to extracellular stresses, such as U.V. irradiation, osmotic stress or inflammatory stimuli (Biondi,2003). MAPKs are serine/threonine kinases that tend to phosphorylate their substrates on S/T-P motif. As the sequence S/T-P is found in ~ 80% of all proteins additional factors are required to direct individual kinases towards the correct substrates (Bardwell,2006).
One way by which the MAPKs ensure their substrate specificity is by interaction through docking motifs, short amino acid stretches located on MAPK-interacting proteins. The classical docking motif is characterized by a cluster of at least two positively charged amino acids followed by by a spacer of 2-to-6 residues from a hydrophobic-X-hydrophobic sequence, where the hydrophobic residues are long-chain aliphatics (usually Leu, Ile). Both in the spacer and in the sequence immediately C-terminal to the hydrophobic-X-hydrophobic element, there is a high propensity for the presence of Pro, Asn, and/or Gly, which are residues that are both turn-forming and helix-breaking (Bardwell,2001). Another type of docking motif is a short peptide containing the sequence FXFP that is usually downstream of the phosphorylation site. This motif was originally found to bind the ERK subfamily but lately it has been shown to promote the interaction with p38alpha and p38 beta2 (Jacobs,1999; Galanis,2001). Substrates can contain the classical docking motif or the FXFP or often contain both. (Sharrocks,2000).
o 8 selected references:

o 7 GO-Terms:

o 15 Instances for DOC_MAPK_1
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
Acc., Gene-, NameStartEndSubsequenceLogic#Ev.OrganismNotes
P06784 STE7
9 19 KTLQRRNLKGLNLNLHPDVG TP 3 Saccharomyces cerevisiae (Baker"s yeast)
P38590 MSG5
29 38 RSLQNRNTKNLSLDIAALHP TP 3 Saccharomyces cerevisiae (Baker"s yeast)
P28562 DUSP1
54 62 STIVRRRAKGAMGLEHIVPN TP 2 Homo sapiens (Human)
Q16828 DUSP6
64 73 PGIMLRRLQKGNLPVRALFT TP 3 Homo sapiens (Human)
P53355 DAPK1
1385 1393 LRELGRRDAADFLLKASSVF FP 1 Homo sapiens (Human)
P19419 ELK1
312 321 ISQPQKGRKPRDLELPLSPS TP 1 Homo sapiens (Human)
P28324 ELK4
318 327 VNNSSRSKKPKGLELAPTLV TP 2 Homo sapiens (Human)
P05412 JUN
32 42 YSNPKILKQSMTLNLADPVG TP 2 Homo sapiens (Human)
Q02078 MEF2A
269 277 LGMNSRKPDLRVVIPPSSKG TP 2 Homo sapiens (Human)
P45985 MAP2K4
40 48 SSMQGKRKALKLNFANPPFK TP 2 Homo sapiens (Human)
P06784 STE7
62 71 SNLFLRRGIKKKLTLDAFGD TP 3 Saccharomyces cerevisiae (Baker"s yeast)
Q03063 DIG1
97 107 KKSLKRGRVPAPLNLSDSNT TP 1 Saccharomyces cerevisiae (Baker"s yeast)
Q03373 DIG2
100 110 NHSLKRKRVPPALNFSDIQA TP 1 Saccharomyces cerevisiae (Baker"s yeast)
P41971 Elk3
292 301 PSLPPKGKKPKGLEISAPQL TP 1 Mus musculus (House mouse)
Q02750 MAP2K1
3 11 MPKKKPTPIQLNPAPDGSAV TP 3 Homo sapiens (Human)
Please cite: ELM 2016-data update and new functionality of the eukaryotic linear motif resource. (PMID:26615199)

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