LIG_CYCLIN_1
ELM server details
ELM
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Functional site class:
 Cyclin recognition site
Functional site description:
 Functional site that interacts with cyclin, and thereby increases the specificity of phosphorylation by cyclin/CDK complexes.
ELM(s): LIG_CYCLIN_1
LIG_CYCLIN_1 description: Substrate recognition site that interacts with cyclin and thereby increases phosphorylation by cyclin/cdk complexes. Predicted protein should have the MOD_CDK site. Also used by cyclin inhibitors.
Pattern: [RK].L.{0,1}[FYLIVMP]
Present in taxon(s): Eukaryota  
Not represented in taxon(s):

o See instances for LIG_CYCLIN_1


o Abstract

The cyclin recognition site (alias Cy or RxL motif) is found in a wide range of cyclin/CDK interacting proteins (PMID:11067844). The presence of this motif in CDK substrates substantially increases the level of phosphorylation at (ST)Px(KR) motifs. Example proteins are the retinoblastoma protein, E2F 1-3 and p53. CDK phosphorylation mainly occurs in the nucleus but there also is some evidence for cytoplasmic function. For example, the cytoplasmic SRC and TAU proteins are known cyclin/CDK targets. The motif is recognised by a conserved region in the cyclin protein and binds in a similar manner as the p21Kip cyclin inhibitor (PDB:1JSU).

o Selected references

Lowe ED, Tews I, Cheng KY, Brown NR, Gul S, Noble ME, Gamblin SJ, Johnson LN
Specificity Determinants of Recruitment Peptides Bound to Phospho-CDK2/Cyclin A(,).
Biochemistry 2002 Dec 31;41(52) : 15625-34.
PMID: 12501191

Russo AA, Jeffrey PD, Patten AK, Massague J, Pavletich NP
Crystal structure of the p27Kip1 cyclin-dependent-kinase inhibitor bound to the cyclin A-Cdk2 complex.
Nature 1996 Jul 25;382(6589) : 325-31.
PMID: 8684460

Takeda DY, Wohlschlegel JA, Dutta A
A bipartite substrate recognition motif for cyclin-dependent kinases.
J Biol Chem 2001 Jan 19;276(3) : 1993-7.
PMID: 11067844

o This ELM has been assigned the following Gene Ontology (GO) terms for biological process, cellular component and molecular function.

Biological Process
  cell cycle
Cellular Component
  cytosol
  nucleus
Molecular Function
  transcription regulator
  cyclin binding activity

 

o Instances for LIG_CYCLIN_1

SequencePositionSubsequence
(Click for evidence information)		PDBGene NameProtein DescriptionOrganism
ORC6_YEAST 178-182 PSITRRKLAFEEDEDE - Name=ORC6; Synonyms=AAP1; OrderedLocusNames=YHR118C; Origin recognition complex subunit 6 (Origin recognition complex 50 kDa subunit) (ACS-associated protein 1). Saccharomyces cerevisiae (Baker's yeast).
P53_HUMAN 381-385 STSRHKKLMFKTEGPD - Name=TP53; Synonyms=P53; Cellular tumor antigen p53 (Tumor suppressor p53) (Phosphoprotein p53) (Antigen NY-CO-13). Homo sapiens (Human).
RB_HUMAN 873-877 PPKPLKKLRFDIEGSD - Name=RB1; Retinoblastoma-associated protein (PP110) (P105-RB) (RB). Homo sapiens (Human).
RBL1_HUMAN 658-661 AGSAKRRLFGEDPPK - Name=RBL1; Retinoblastoma-like protein 1 (107 kDa retinoblastoma-associated protein) (p107) (PRB1). Homo sapiens (Human).
RBL2_HUMAN 680-684 ASTTRRRLFVENDSPS - Name=RBL2; Synonyms=RB2; Retinoblastoma-like protein 2 (130 kDa retinoblastoma-associated protein) (p130) (PRB2) (RBR-2). Homo sapiens (Human).
HIRA_HUMAN 629-633 SSLSKRKLELEVETVE - Name=HIRA; Synonyms=DGCR1, HIR, TUPLE1; Protein HIRA (TUP1-like enhancer of split protein 1). Homo sapiens (Human).
CUTL1_HUMAN 1301-1305 RSRIRRELFIEEIQAG - Name=CUTL1; Homeobox protein cut-like 1 (CCAAT displacement protein) (CDP). Homo sapiens (Human).
PMYT1_HUMAN 486-489 PSFEPRNLLSMFEDT - Name=PKMYT1; Synonyms=MYT1; Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase (EC 2.7.11.1) (Myt1 kinase). Homo sapiens (Human).
CDC6_HUMAN 94-98 HTLKGRRLVFDNQLTI - Name=CDC6; Synonyms=CDC18L; Cell division control protein 6 homolog (CDC6-related protein) (p62(cdc6)) (HsCDC6) (HsCDC18). Homo sapiens (Human).
MPIP1_HUMAN 10-14 PSPAPRRLLFACSPPP - Name=CDC25A; M-phase inducer phosphatase 1 (EC 3.1.3.48) (Dual specificity phosphatase Cdc25A). Homo sapiens (Human).
Q13632_HUMAN 1062-1066 KPCHRRVLCFDSTTAP - Name=NPAT; NPAT protein. Homo sapiens (Human).
CDN1A_HUMAN 19-22 GSKACRRLFGPVDSE - Name=CDKN1A; Synonyms=CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1; Cyclin-dependent kinase inhibitor 1 (p21) (CDK-interacting protein 1) (Melanoma differentiation-associated protein 6) (MDA-6). Homo sapiens (Human).
CDN1A_HUMAN 155-159 FYHSKRRLIFSKRKP - Name=CDKN1A; Synonyms=CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1; Cyclin-dependent kinase inhibitor 1 (p21) (CDK-interacting protein 1) (Melanoma differentiation-associated protein 6) (MDA-6). Homo sapiens (Human).
CDN1B_HUMAN 30-33 KPSACRNLFGPVDHE - Name=CDKN1B; Synonyms=KIP1; Cyclin-dependent kinase inhibitor 1B (Cyclin-dependent kinase inhibitor p27) (p27Kip1). Homo sapiens (Human).
CDN1C_HUMAN 31-34 RTSACRSLFGPVDHE - Name=CDKN1C; Synonyms=KIP2; Cyclin-dependent kinase inhibitor 1C (Cyclin-dependent kinase inhibitor p57) (p57KIP2). Homo sapiens (Human).
H10_HUMAN 72-75 IKLSIKRLVTTGVLK - Name=H1F0; Synonyms=H1FV; Histone H1.0 (Histone H1(0)) (Histone H1'). Homo sapiens (Human).
Q8UWJ8_CHICK 394-398 TGHSYRVLYLAMSPDG - - CDH1-A. Gallus gallus (Chicken).
CENPR_HUMAN 5-9 MPVKRSLKLDGLLEE - Name=ITGB3BP; Synonyms=CENPR, NRIF3; Centromere protein R (CENP-R) (Nuclear receptor-interacting factor 3) (Integrin beta-3-binding protein) (Beta3-endonexin). Homo sapiens (Human).
RUX_DROME 248-251 RRCVRRTLFTEENTQ - Name=rux; ORFNames=CG4336; Cell cycle negative regulator roughex. Drosophila melanogaster (Fruit fly).
E2F1_HUMAN 90-94 RPPVKRRLDLETDHQY - Name=E2F1; Synonyms=RBBP3; Transcription factor E2F1 (E2F-1) (Retinoblastoma-binding protein 3) (RBBP-3) (PRB-binding protein E2F-1) (PBR3) (Retinoblastoma-associated protein 1) (RBAP-1). Homo sapiens (Human).
E2F2_HUMAN 87-91 RLPAKRKLDLEGIGRP - Name=E2F2; Transcription factor E2F2 (E2F-2). Homo sapiens (Human).
E2F3_HUMAN 134-138 GPPAKRRLELGESGHQ - Name=E2F3; Synonyms=KIAA0075; Transcription factor E2F3 (E2F-3). Homo sapiens (Human).
AKA12_MOUSE 501-504 QGSPLKKLFSSSGLK - Name=Akap12; Synonyms=Gag12, Ssecks; A-kinase anchor protein 12 (Src-suppressed C kinase substrate) (SSeCKS) (Germ cell lineage protein gercelin). Mus musculus (Mouse).